PubMed:19506294
Annnotations
Glycan-Motif
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 350-353 | https://glytoucan.org/Structures/Glycans/G61168WC | denotes | GM2 |
| T2 | 350-353 | https://glytoucan.org/Structures/Glycans/G79389NT | denotes | GM2 |
| T3 | 564-567 | https://glytoucan.org/Structures/Glycans/G61168WC | denotes | GM2 |
| T4 | 564-567 | https://glytoucan.org/Structures/Glycans/G79389NT | denotes | GM2 |
| T5 | 1680-1683 | https://glytoucan.org/Structures/Glycans/G61168WC | denotes | GM2 |
| T6 | 1680-1683 | https://glytoucan.org/Structures/Glycans/G79389NT | denotes | GM2 |
| T7 | 1893-1896 | https://glytoucan.org/Structures/Glycans/G61168WC | denotes | GM2 |
| T8 | 1893-1896 | https://glytoucan.org/Structures/Glycans/G79389NT | denotes | GM2 |
GlyCosmos6-Glycan-Motif-Image
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 350-353 | Glycan_Motif | denotes | GM2 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G79389NT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G61168WC |
| T3 | 564-567 | Glycan_Motif | denotes | GM2 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G79389NT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G61168WC |
| T5 | 1680-1683 | Glycan_Motif | denotes | GM2 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G79389NT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G61168WC |
| T7 | 1893-1896 | Glycan_Motif | denotes | GM2 | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G79389NT|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G61168WC |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| TextSentencer_T1 | 0-133 | Sentence | denotes | Production of human beta-hexosaminidase A with highly phosphorylated N-glycans by the overexpression of the Ogataea minuta MNN4 gene. |
| TextSentencer_T2 | 134-269 | Sentence | denotes | Effective enzyme replacement therapy for lysosomal storage diseases requires a recombinant enzyme with highly phosphorylated N-glycans. |
| TextSentencer_T3 | 270-369 | Sentence | denotes | Recombinant human beta-hexosaminidase A is a potentially therapeutic enzyme for GM2-gangliosidosis. |
| TextSentencer_T4 | 370-592 | Sentence | denotes | Recombinant HexA has been produced by using the methylotrophic yeast Ogataea minuta as a host, and the purified enzyme was tested for its replacement effect on cultured fibroblasts derived from GM2-gangliosidosis patients. |
| TextSentencer_T5 | 593-825 | Sentence | denotes | Although the therapeutic effect was observed, in order to obtain the higher therapeutic effect with a little dose as possible, increased phosphorylation of recombinant beta-hexosaminidase A N-glycans is suggested to be prerequisite. |
| TextSentencer_T6 | 826-1014 | Sentence | denotes | In the budding yeast Saccharomyces cerevisiae, the overexpression of MNN4, which encodes a positive regulator of mannosylphosphate transferase, led to increased mannosylphosphate contents. |
| TextSentencer_T7 | 1015-1127 | Sentence | denotes | In the present study, we cloned OmMNN4, a homologous gene to ScMNN4, based on the genomic sequence of O. minuta. |
| TextSentencer_T8 | 1128-1261 | Sentence | denotes | We overexpressed the cloned gene under the control of the alcohol oxidase promoter in a beta-hexosaminidase A-producing yeast strain. |
| TextSentencer_T9 | 1262-1489 | Sentence | denotes | Structural analysis of pyridylamine-labeled N-glycans by high-performance liquid chromatography revealed that the overexpression of MNN4 caused a 3-fold increase in phosphorylated N-glycans of recombinant beta-hexosaminidase A. |
| TextSentencer_T10 | 1490-1730 | Sentence | denotes | The recombinant enzyme prepared from strains overexpressing OmMNN4 was more effectively incorporated into cultured fibroblasts and neural cells, and it more rapidly degraded the accumulated GM2-ganglioside as compared to the control enzyme. |
| TextSentencer_T11 | 1731-1912 | Sentence | denotes | These results suggest that beta-hexosaminidase A produced in a strain that overexpresses OmMNN4 will act as an effective enzyme for use in replacement therapy of GM2-gangliosidosis. |
| T1 | 0-133 | Sentence | denotes | Production of human beta-hexosaminidase A with highly phosphorylated N-glycans by the overexpression of the Ogataea minuta MNN4 gene. |
| T2 | 134-269 | Sentence | denotes | Effective enzyme replacement therapy for lysosomal storage diseases requires a recombinant enzyme with highly phosphorylated N-glycans. |
| T3 | 270-369 | Sentence | denotes | Recombinant human beta-hexosaminidase A is a potentially therapeutic enzyme for GM2-gangliosidosis. |
| T4 | 370-592 | Sentence | denotes | Recombinant HexA has been produced by using the methylotrophic yeast Ogataea minuta as a host, and the purified enzyme was tested for its replacement effect on cultured fibroblasts derived from GM2-gangliosidosis patients. |
| T5 | 593-825 | Sentence | denotes | Although the therapeutic effect was observed, in order to obtain the higher therapeutic effect with a little dose as possible, increased phosphorylation of recombinant beta-hexosaminidase A N-glycans is suggested to be prerequisite. |
| T6 | 826-1014 | Sentence | denotes | In the budding yeast Saccharomyces cerevisiae, the overexpression of MNN4, which encodes a positive regulator of mannosylphosphate transferase, led to increased mannosylphosphate contents. |
| T7 | 1015-1127 | Sentence | denotes | In the present study, we cloned OmMNN4, a homologous gene to ScMNN4, based on the genomic sequence of O. minuta. |
| T8 | 1128-1261 | Sentence | denotes | We overexpressed the cloned gene under the control of the alcohol oxidase promoter in a beta-hexosaminidase A-producing yeast strain. |
| T9 | 1262-1730 | Sentence | denotes | Structural analysis of pyridylamine-labeled N-glycans by high-performance liquid chromatography revealed that the overexpression of MNN4 caused a 3-fold increase in phosphorylated N-glycans of recombinant beta-hexosaminidase A. The recombinant enzyme prepared from strains overexpressing OmMNN4 was more effectively incorporated into cultured fibroblasts and neural cells, and it more rapidly degraded the accumulated GM2-ganglioside as compared to the control enzyme. |
| T10 | 1731-1912 | Sentence | denotes | These results suggest that beta-hexosaminidase A produced in a strain that overexpresses OmMNN4 will act as an effective enzyme for use in replacement therapy of GM2-gangliosidosis. |
| T1 | 0-133 | Sentence | denotes | Production of human beta-hexosaminidase A with highly phosphorylated N-glycans by the overexpression of the Ogataea minuta MNN4 gene. |
| T2 | 134-269 | Sentence | denotes | Effective enzyme replacement therapy for lysosomal storage diseases requires a recombinant enzyme with highly phosphorylated N-glycans. |
| T3 | 270-369 | Sentence | denotes | Recombinant human beta-hexosaminidase A is a potentially therapeutic enzyme for GM2-gangliosidosis. |
| T4 | 370-592 | Sentence | denotes | Recombinant HexA has been produced by using the methylotrophic yeast Ogataea minuta as a host, and the purified enzyme was tested for its replacement effect on cultured fibroblasts derived from GM2-gangliosidosis patients. |
| T5 | 593-825 | Sentence | denotes | Although the therapeutic effect was observed, in order to obtain the higher therapeutic effect with a little dose as possible, increased phosphorylation of recombinant beta-hexosaminidase A N-glycans is suggested to be prerequisite. |
| T6 | 826-1014 | Sentence | denotes | In the budding yeast Saccharomyces cerevisiae, the overexpression of MNN4, which encodes a positive regulator of mannosylphosphate transferase, led to increased mannosylphosphate contents. |
| T7 | 1015-1127 | Sentence | denotes | In the present study, we cloned OmMNN4, a homologous gene to ScMNN4, based on the genomic sequence of O. minuta. |
| T8 | 1128-1261 | Sentence | denotes | We overexpressed the cloned gene under the control of the alcohol oxidase promoter in a beta-hexosaminidase A-producing yeast strain. |
| T9 | 1262-1489 | Sentence | denotes | Structural analysis of pyridylamine-labeled N-glycans by high-performance liquid chromatography revealed that the overexpression of MNN4 caused a 3-fold increase in phosphorylated N-glycans of recombinant beta-hexosaminidase A. |
| T10 | 1490-1730 | Sentence | denotes | The recombinant enzyme prepared from strains overexpressing OmMNN4 was more effectively incorporated into cultured fibroblasts and neural cells, and it more rapidly degraded the accumulated GM2-ganglioside as compared to the control enzyme. |
| T11 | 1731-1912 | Sentence | denotes | These results suggest that beta-hexosaminidase A produced in a strain that overexpresses OmMNN4 will act as an effective enzyme for use in replacement therapy of GM2-gangliosidosis. |
GlyCosmos6-Glycan-Motif-Structure
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 350-353 | https://glytoucan.org/Structures/Glycans/G61168WC | denotes | GM2 |
| T2 | 350-353 | https://glytoucan.org/Structures/Glycans/G79389NT | denotes | GM2 |
| T3 | 564-567 | https://glytoucan.org/Structures/Glycans/G61168WC | denotes | GM2 |
| T4 | 564-567 | https://glytoucan.org/Structures/Glycans/G79389NT | denotes | GM2 |
| T5 | 1680-1683 | https://glytoucan.org/Structures/Glycans/G61168WC | denotes | GM2 |
| T6 | 1680-1683 | https://glytoucan.org/Structures/Glycans/G79389NT | denotes | GM2 |
| T7 | 1893-1896 | https://glytoucan.org/Structures/Glycans/G61168WC | denotes | GM2 |
| T8 | 1893-1896 | https://glytoucan.org/Structures/Glycans/G79389NT | denotes | GM2 |
GlycoBiology-GDGDB
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| _T1 | 382-386 | http://acgg.asia/db/diseases/gdgdb?con_ui=CON00055 | denotes | HexA |
| _T2 | 382-386 | http://acgg.asia/db/diseases/gdgdb?con_ui=CON00056 | denotes | HexA |
| _T3 | 382-386 | http://acgg.asia/db/diseases/gdgdb?con_ui=CON00057 | denotes | HexA |
| _T4 | 350-368 | http://acgg.asia/db/diseases/gdgdb?con_ui=CON00055 | denotes | GM2-gangliosidosis |
| _T5 | 564-582 | http://acgg.asia/db/diseases/gdgdb?con_ui=CON00055 | denotes | GM2-gangliosidosis |
| _T6 | 1893-1911 | http://acgg.asia/db/diseases/gdgdb?con_ui=CON00055 | denotes | GM2-gangliosidosis |
GlycoBiology-PACDB
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| _T1 | 847-871 | http://acgg.asia/db/diseases/pacdb/lec?ids=LEC297 | denotes | Saccharomyces cerevisiae |
ICD10
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 350-368 | http://purl.bioontology.org/ontology/ICD10/E75.0 | denotes | GM2-gangliosidosis |
| T2 | 564-582 | http://purl.bioontology.org/ontology/ICD10/E75.0 | denotes | GM2-gangliosidosis |
| T3 | 1893-1911 | http://purl.bioontology.org/ontology/ICD10/E75.0 | denotes | GM2-gangliosidosis |
GlycoBiology-FMA
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| _T1 | 128-132 | FMAID:198663 | denotes | gene |
| _T2 | 175-184 | FMAID:63836 | denotes | lysosomal |
| _T3 | 175-184 | FMAID:162299 | denotes | lysosomal |
| _T4 | 354-368 | FMAID:82816 | denotes | gangliosidosis |
| _T5 | 354-368 | FMAID:196811 | denotes | gangliosidosis |
| _T6 | 493-499 | FMAID:178661 | denotes | tested |
| _T7 | 539-550 | FMAID:63877 | denotes | fibroblasts |
| _T8 | 539-550 | FMAID:162340 | denotes | fibroblasts |
| _T9 | 568-582 | FMAID:82816 | denotes | gangliosidosis |
| _T10 | 568-582 | FMAID:196811 | denotes | gangliosidosis |
| _T11 | 1068-1072 | FMAID:198663 | denotes | gene |
| _T12 | 1097-1104 | FMAID:198062 | denotes | genomic |
| _T13 | 1097-1104 | FMAID:84116 | denotes | genomic |
| _T14 | 1156-1160 | FMAID:198663 | denotes | gene |
| _T15 | 1336-1342 | FMAID:85815 | denotes | liquid |
| _T16 | 1605-1616 | FMAID:162340 | denotes | fibroblasts |
| _T17 | 1605-1616 | FMAID:63877 | denotes | fibroblasts |
| _T18 | 1621-1633 | FMAID:201593 | denotes | neural cells |
| _T19 | 1621-1633 | FMAID:201610 | denotes | neural cells |
| _T20 | 1621-1633 | FMAID:86684 | denotes | neural cells |
| _T21 | 1621-1633 | FMAID:174560 | denotes | neural cells |
| _T22 | 1621-1633 | FMAID:72550 | denotes | neural cells |
| _T23 | 1621-1633 | FMAID:70333 | denotes | neural cells |
| _T24 | 1621-1633 | FMAID:171175 | denotes | neural cells |
| _T25 | 1621-1633 | FMAID:86667 | denotes | neural cells |
| _T26 | 1628-1633 | FMAID:169002 | denotes | cells |
| _T27 | 1628-1633 | FMAID:68646 | denotes | cells |
| _T28 | 1684-1695 | FMAID:82816 | denotes | ganglioside |
| _T29 | 1684-1695 | FMAID:196811 | denotes | ganglioside |
| _T30 | 1897-1911 | FMAID:82816 | denotes | gangliosidosis |
| _T31 | 1897-1911 | FMAID:196811 | denotes | gangliosidosis |
uniprot-human
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 20-39 | http://www.uniprot.org/uniprot/Q9HAR0 | denotes | beta-hexosaminidase |
| T2 | 288-307 | http://www.uniprot.org/uniprot/Q9HAR0 | denotes | beta-hexosaminidase |
| T3 | 761-780 | http://www.uniprot.org/uniprot/Q9HAR0 | denotes | beta-hexosaminidase |
| T4 | 1216-1235 | http://www.uniprot.org/uniprot/Q9HAR0 | denotes | beta-hexosaminidase |
| T5 | 1467-1486 | http://www.uniprot.org/uniprot/Q9HAR0 | denotes | beta-hexosaminidase |
| T6 | 1758-1777 | http://www.uniprot.org/uniprot/Q9HAR0 | denotes | beta-hexosaminidase |
| T7 | 957-968 | http://www.uniprot.org/uniprot/Q99484 | denotes | transferase |
uniprot-mouse
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 20-39 | http://www.uniprot.org/uniprot/Q9EQQ9 | denotes | beta-hexosaminidase |
| T2 | 288-307 | http://www.uniprot.org/uniprot/Q9EQQ9 | denotes | beta-hexosaminidase |
| T3 | 761-780 | http://www.uniprot.org/uniprot/Q9EQQ9 | denotes | beta-hexosaminidase |
| T4 | 1216-1235 | http://www.uniprot.org/uniprot/Q9EQQ9 | denotes | beta-hexosaminidase |
| T5 | 1467-1486 | http://www.uniprot.org/uniprot/Q9EQQ9 | denotes | beta-hexosaminidase |
| T6 | 1758-1777 | http://www.uniprot.org/uniprot/Q9EQQ9 | denotes | beta-hexosaminidase |
| T7 | 957-968 | http://www.uniprot.org/uniprot/P38649 | denotes | transferase |
GlycoBiology-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 20-24 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
| T2 | 20-24 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
| T3 | 108-115 | http://purl.bioontology.org/ontology/NCBITAXON/461281 | denotes | Ogataea |
| T4 | 108-122 | http://purl.bioontology.org/ontology/NCBITAXON/36026 | denotes | Ogataea minuta |
| T5 | 288-292 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
| T6 | 288-292 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
| T7 | 439-446 | http://purl.bioontology.org/ontology/NCBITAXON/461281 | denotes | Ogataea |
| T8 | 439-453 | http://purl.bioontology.org/ontology/NCBITAXON/36026 | denotes | Ogataea minuta |
| T9 | 761-765 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
| T10 | 761-765 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
| T11 | 847-860 | http://purl.bioontology.org/ontology/NCBITAXON/4891 | denotes | Saccharomyces |
| T12 | 847-860 | http://purl.bioontology.org/ontology/NCBITAXON/36034 | denotes | Saccharomyces |
| T13 | 847-860 | http://purl.bioontology.org/ontology/NCBITAXON/4930 | denotes | Saccharomyces |
| T14 | 847-860 | http://purl.bioontology.org/ontology/NCBITAXON/4895 | denotes | Saccharomyces |
| T15 | 1216-1220 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
| T16 | 1216-1220 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
| T17 | 1467-1471 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
| T18 | 1467-1471 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
| T19 | 1628-1633 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
| T20 | 1758-1762 | http://purl.bioontology.org/ontology/NCBITAXON/158455 | denotes | beta |
| T21 | 1758-1762 | http://purl.bioontology.org/ontology/NCBITAXON/3554 | denotes | beta |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 25-39 | http://purl.obolibrary.org/obo/GO_0004563 | denotes | hexosaminidase |
| T2 | 293-307 | http://purl.obolibrary.org/obo/GO_0004563 | denotes | hexosaminidase |
| T3 | 766-780 | http://purl.obolibrary.org/obo/GO_0004563 | denotes | hexosaminidase |
| T4 | 1221-1235 | http://purl.obolibrary.org/obo/GO_0004563 | denotes | hexosaminidase |
| T5 | 1472-1486 | http://purl.obolibrary.org/obo/GO_0004563 | denotes | hexosaminidase |
| T6 | 1763-1777 | http://purl.obolibrary.org/obo/GO_0004563 | denotes | hexosaminidase |
| T7 | 54-68 | http://purl.obolibrary.org/obo/GO_0016310 | denotes | phosphorylated |
| T8 | 244-258 | http://purl.obolibrary.org/obo/GO_0016310 | denotes | phosphorylated |
| T9 | 730-745 | http://purl.obolibrary.org/obo/GO_0016310 | denotes | phosphorylation |
| T10 | 185-192 | http://purl.obolibrary.org/obo/GO_0051235 | denotes | storage |
| T11 | 185-192 | http://purl.obolibrary.org/obo/GO_0035732 | denotes | storage |
| T12 | 833-840 | http://purl.obolibrary.org/obo/GO_0007114 | denotes | budding |
| T13 | 926-935 | http://purl.obolibrary.org/obo/GO_0065007 | denotes | regulator |
| T14 | 1655-1663 | http://purl.obolibrary.org/obo/GO_0009056 | denotes | degraded |
GO-CC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 175-184 | http://purl.obolibrary.org/obo/GO_0005764 | denotes | lysosomal |
| T2 | 459-463 | http://purl.obolibrary.org/obo/GO_0018995 | denotes | host |
| T3 | 1628-1633 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
GlyTouCan-IUPAC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| GlycanIUPAC_T1 | 382-386 | "http://rdf.glycoinfo.org/glycan/G81168AE" | denotes | HexA |
| GlycanIUPAC_T2 | 382-386 | "http://rdf.glycoinfo.org/glycan/G90684IR" | denotes | HexA |
| GlycanIUPAC_T3 | 382-386 | "http://rdf.glycoinfo.org/glycan/G32900AW" | denotes | HexA |
| GlycanIUPAC_T4 | 382-386 | "http://rdf.glycoinfo.org/glycan/G72029GV" | denotes | HexA |
| GlycanIUPAC_T5 | 382-386 | "http://rdf.glycoinfo.org/glycan/G69792ST" | denotes | HexA |
| GlycanIUPAC_T6 | 382-386 | "http://rdf.glycoinfo.org/glycan/G18081CC" | denotes | HexA |
| GlycanIUPAC_T7 | 382-386 | "http://rdf.glycoinfo.org/glycan/G39201OW" | denotes | HexA |
GlycoBiology-Motifs
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 69-78 | http://rdf.glycoinfo.org/glycan/G00027MO | denotes | N-glycans |
| T2 | 259-268 | http://rdf.glycoinfo.org/glycan/G00027MO | denotes | N-glycans |
| T3 | 783-792 | http://rdf.glycoinfo.org/glycan/G00027MO | denotes | N-glycans |
| T4 | 1306-1315 | http://rdf.glycoinfo.org/glycan/G00027MO | denotes | N-glycans |
| T5 | 1442-1451 | http://rdf.glycoinfo.org/glycan/G00027MO | denotes | N-glycans |
GlycoBiology-Epitope
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| PD-GlycoEpitope-B_T1 | 1298-1305 | id | denotes | labeled |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 350-353 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T2 | 564-567 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T3 | 1680-1683 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T4 | 1893-1896 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
mondo_disease
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 175-201 | Disease | denotes | lysosomal storage diseases | http://purl.obolibrary.org/obo/MONDO_0002561 |
| T2 | 350-368 | Disease | denotes | GM2-gangliosidosis | http://purl.obolibrary.org/obo/MONDO_0017720 |
| T3 | 564-582 | Disease | denotes | GM2-gangliosidosis | http://purl.obolibrary.org/obo/MONDO_0017720 |
| T4 | 1893-1911 | Disease | denotes | GM2-gangliosidosis | http://purl.obolibrary.org/obo/MONDO_0017720 |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 350-353 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T2 | 564-567 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T3 | 1680-1683 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
| T4 | 1893-1896 | Glycan | denotes | GM2 | https://api.glycosmos.org/wurcs2image/latest/png/binary/G79389NT |
GlyCosmos15-CL
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 539-550 | Cell | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL:0000057 |
| T2 | 1605-1616 | Cell | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL:0000057 |
| T3 | 1621-1633 | Cell | denotes | neural cells | http://purl.obolibrary.org/obo/CL:0002319 |
GlyCosmos15-MONDO
| Id | Subject | Object | Predicate | Lexical cue | mondo_id |
|---|---|---|---|---|---|
| T1 | 175-201 | Disease | denotes | lysosomal storage diseases | MONDO:0002561 |
| T2 | 350-368 | Disease | denotes | GM2-gangliosidosis | MONDO:0017720 |
| T3 | 564-582 | Disease | denotes | GM2-gangliosidosis | MONDO:0017720 |
| T4 | 1893-1911 | Disease | denotes | GM2-gangliosidosis | MONDO:0017720 |
GlyCosmos15-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 539-550 | Body_part | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL_0000057 |
| T2 | 1605-1616 | Body_part | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL_0000057 |
| T3 | 1621-1633 | Body_part | denotes | neural cells | http://purl.obolibrary.org/obo/CL_0002319 |
GlyCosmos15-Taxon
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 14-19 | Organism | denotes | human | 9606 |
| T2 | 108-122 | Organism | denotes | Ogataea minuta | 36026 |
| T3 | 282-287 | Organism | denotes | human | 9606 |
| T4 | 439-453 | Organism | denotes | Ogataea minuta | 36026 |
| T5 | 583-591 | Organism | denotes | patients | 9606 |
| T6 | 847-871 | Organism | denotes | Saccharomyces cerevisiae | 4932 |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-133 | Sentence | denotes | Production of human beta-hexosaminidase A with highly phosphorylated N-glycans by the overexpression of the Ogataea minuta MNN4 gene. |
| T2 | 134-269 | Sentence | denotes | Effective enzyme replacement therapy for lysosomal storage diseases requires a recombinant enzyme with highly phosphorylated N-glycans. |
| T3 | 270-369 | Sentence | denotes | Recombinant human beta-hexosaminidase A is a potentially therapeutic enzyme for GM2-gangliosidosis. |
| T4 | 370-592 | Sentence | denotes | Recombinant HexA has been produced by using the methylotrophic yeast Ogataea minuta as a host, and the purified enzyme was tested for its replacement effect on cultured fibroblasts derived from GM2-gangliosidosis patients. |
| T5 | 593-825 | Sentence | denotes | Although the therapeutic effect was observed, in order to obtain the higher therapeutic effect with a little dose as possible, increased phosphorylation of recombinant beta-hexosaminidase A N-glycans is suggested to be prerequisite. |
| T6 | 826-1014 | Sentence | denotes | In the budding yeast Saccharomyces cerevisiae, the overexpression of MNN4, which encodes a positive regulator of mannosylphosphate transferase, led to increased mannosylphosphate contents. |
| T7 | 1015-1127 | Sentence | denotes | In the present study, we cloned OmMNN4, a homologous gene to ScMNN4, based on the genomic sequence of O. minuta. |
| T8 | 1128-1261 | Sentence | denotes | We overexpressed the cloned gene under the control of the alcohol oxidase promoter in a beta-hexosaminidase A-producing yeast strain. |
| T9 | 1262-1730 | Sentence | denotes | Structural analysis of pyridylamine-labeled N-glycans by high-performance liquid chromatography revealed that the overexpression of MNN4 caused a 3-fold increase in phosphorylated N-glycans of recombinant beta-hexosaminidase A. The recombinant enzyme prepared from strains overexpressing OmMNN4 was more effectively incorporated into cultured fibroblasts and neural cells, and it more rapidly degraded the accumulated GM2-ganglioside as compared to the control enzyme. |
| T10 | 1731-1912 | Sentence | denotes | These results suggest that beta-hexosaminidase A produced in a strain that overexpresses OmMNN4 will act as an effective enzyme for use in replacement therapy of GM2-gangliosidosis. |
GlyCosmos15-FMA
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 175-184 | Body_part | denotes | lysosomal | FMA:63836 |
| T2 | 539-550 | Body_part | denotes | fibroblasts | FMA:63877 |
| T3 | 1605-1616 | Body_part | denotes | fibroblasts | FMA:63877 |
| T4 | 1621-1633 | Body_part | denotes | neural cells | FMA:70333 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 14-19 | OrganismTaxon | denotes | human | 9606 |
| T2 | 108-122 | OrganismTaxon | denotes | Ogataea minuta | 36026 |
| T3 | 282-287 | OrganismTaxon | denotes | human | 9606 |
| T4 | 439-453 | OrganismTaxon | denotes | Ogataea minuta | 36026 |
| T5 | 847-871 | OrganismTaxon | denotes | Saccharomyces cerevisiae | 4932 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 539-550 | Body_part | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL_0000057 |
| T2 | 1605-1616 | Body_part | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL_0000057 |
| T3 | 1621-1633 | Body_part | denotes | neural cells | http://purl.obolibrary.org/obo/CL_0002319 |
CL-cell
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 539-550 | Cell | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL:0000057 |
| T2 | 1605-1616 | Cell | denotes | fibroblasts | http://purl.obolibrary.org/obo/CL:0000057 |
| T3 | 1621-1633 | Cell | denotes | neural cells | http://purl.obolibrary.org/obo/CL:0002319 |