PubMed:19494346
Annnotations
Glycosmos6-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 739-744 | http://purl.obolibrary.org/obo/MAT_0000097 | denotes | liver |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| TextSentencer_T1 | 0-109 | Sentence | denotes | Establishment of a real-time analytical method for free oligosaccharide transport from the ER to the cytosol. |
| TextSentencer_T2 | 110-261 | Sentence | denotes | During N-glycosylation of proteins, significant amounts of free unconjugated glycans are also generated in the lumen of the endoplasmic reticulum (ER). |
| TextSentencer_T3 | 262-394 | Sentence | denotes | These ER-derived free glycans are translocated into the cytosol by a putative transporter on the ER membrane for further processing. |
| TextSentencer_T4 | 395-469 | Sentence | denotes | However, the molecular nature of the transporter remains to be determined. |
| TextSentencer_T5 | 470-652 | Sentence | denotes | Here, we report the establishment of a novel assay method for free oligosaccharide transport from the ER lumen using chemically synthesized fluorescence-labeled N-glycan derivatives. |
| TextSentencer_T6 | 653-860 | Sentence | denotes | In this method, fluorescence-labeled glycan substrates were encapsulated inside mouse liver microsomes, followed by incubation with the cytosol and a fluorescence-quenching agent (anti-fluorophore antibody). |
| TextSentencer_T7 | 861-968 | Sentence | denotes | The rate of substrate efflux was then monitored in real time by the decrease in the fluorescence intensity. |
| TextSentencer_T8 | 969-1121 | Sentence | denotes | The present data clearly demonstrated that the oligosaccharide transport activity under the current assay conditions was both ATP and cytosol dependent. |
| TextSentencer_T9 | 1122-1280 | Sentence | denotes | The transporter activity was also found to be glycan structure specific because free glucosylated glycans were unable to be transported out of the microsomes. |
| TextSentencer_T10 | 1281-1384 | Sentence | denotes | This new assay method will be a useful tool for identifying the transporter protein on the ER membrane. |
| T1 | 0-109 | Sentence | denotes | Establishment of a real-time analytical method for free oligosaccharide transport from the ER to the cytosol. |
| T2 | 110-261 | Sentence | denotes | During N-glycosylation of proteins, significant amounts of free unconjugated glycans are also generated in the lumen of the endoplasmic reticulum (ER). |
| T3 | 262-394 | Sentence | denotes | These ER-derived free glycans are translocated into the cytosol by a putative transporter on the ER membrane for further processing. |
| T4 | 395-469 | Sentence | denotes | However, the molecular nature of the transporter remains to be determined. |
| T5 | 470-652 | Sentence | denotes | Here, we report the establishment of a novel assay method for free oligosaccharide transport from the ER lumen using chemically synthesized fluorescence-labeled N-glycan derivatives. |
| T6 | 653-860 | Sentence | denotes | In this method, fluorescence-labeled glycan substrates were encapsulated inside mouse liver microsomes, followed by incubation with the cytosol and a fluorescence-quenching agent (anti-fluorophore antibody). |
| T7 | 861-968 | Sentence | denotes | The rate of substrate efflux was then monitored in real time by the decrease in the fluorescence intensity. |
| T8 | 969-1121 | Sentence | denotes | The present data clearly demonstrated that the oligosaccharide transport activity under the current assay conditions was both ATP and cytosol dependent. |
| T9 | 1122-1280 | Sentence | denotes | The transporter activity was also found to be glycan structure specific because free glucosylated glycans were unable to be transported out of the microsomes. |
| T10 | 1281-1384 | Sentence | denotes | This new assay method will be a useful tool for identifying the transporter protein on the ER membrane. |
| T1 | 0-109 | Sentence | denotes | Establishment of a real-time analytical method for free oligosaccharide transport from the ER to the cytosol. |
| T2 | 110-261 | Sentence | denotes | During N-glycosylation of proteins, significant amounts of free unconjugated glycans are also generated in the lumen of the endoplasmic reticulum (ER). |
| T3 | 262-394 | Sentence | denotes | These ER-derived free glycans are translocated into the cytosol by a putative transporter on the ER membrane for further processing. |
| T4 | 395-469 | Sentence | denotes | However, the molecular nature of the transporter remains to be determined. |
| T5 | 470-652 | Sentence | denotes | Here, we report the establishment of a novel assay method for free oligosaccharide transport from the ER lumen using chemically synthesized fluorescence-labeled N-glycan derivatives. |
| T6 | 653-860 | Sentence | denotes | In this method, fluorescence-labeled glycan substrates were encapsulated inside mouse liver microsomes, followed by incubation with the cytosol and a fluorescence-quenching agent (anti-fluorophore antibody). |
| T7 | 861-968 | Sentence | denotes | The rate of substrate efflux was then monitored in real time by the decrease in the fluorescence intensity. |
| T8 | 969-1121 | Sentence | denotes | The present data clearly demonstrated that the oligosaccharide transport activity under the current assay conditions was both ATP and cytosol dependent. |
| T9 | 1122-1280 | Sentence | denotes | The transporter activity was also found to be glycan structure specific because free glucosylated glycans were unable to be transported out of the microsomes. |
| T10 | 1281-1384 | Sentence | denotes | This new assay method will be a useful tool for identifying the transporter protein on the ER membrane. |
GlycoBiology-FMA
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| _T1 | 56-71 | FMAID:196731 | denotes | oligosaccharide |
| _T2 | 56-71 | FMAID:82742 | denotes | oligosaccharide |
| _T3 | 101-108 | FMAID:256055 | denotes | cytosol |
| _T4 | 136-144 | FMAID:165447 | denotes | proteins |
| _T5 | 136-144 | FMAID:67257 | denotes | proteins |
| _T6 | 221-255 | FMAID:199158 | denotes | lumen of the endoplasmic reticulum |
| _T7 | 221-255 | FMAID:165580 | denotes | lumen of the endoplasmic reticulum |
| _T8 | 221-255 | FMAID:199160 | denotes | lumen of the endoplasmic reticulum |
| _T9 | 221-255 | FMAID:165060 | denotes | lumen of the endoplasmic reticulum |
| _T10 | 221-255 | FMAID:199094 | denotes | lumen of the endoplasmic reticulum |
| _T11 | 221-255 | FMAID:67672 | denotes | lumen of the endoplasmic reticulum |
| _T12 | 234-245 | FMAID:165003 | denotes | endoplasmic |
| _T13 | 234-245 | FMAID:66856 | denotes | endoplasmic |
| _T14 | 234-255 | FMAID:165027 | denotes | endoplasmic reticulum |
| _T15 | 234-255 | FMAID:210694 | denotes | endoplasmic reticulum |
| _T16 | 234-255 | FMAID:165144 | denotes | endoplasmic reticulum |
| _T17 | 234-255 | FMAID:67429 | denotes | endoplasmic reticulum |
| _T18 | 234-255 | FMAID:67434 | denotes | endoplasmic reticulum |
| _T19 | 234-255 | FMAID:165142 | denotes | endoplasmic reticulum |
| _T20 | 234-255 | FMAID:67438 | denotes | endoplasmic reticulum |
| _T21 | 234-255 | FMAID:210679 | denotes | endoplasmic reticulum |
| _T22 | 234-255 | FMAID:211269 | denotes | endoplasmic reticulum |
| _T23 | 234-255 | FMAID:66898 | denotes | endoplasmic reticulum |
| _T24 | 234-255 | FMAID:212510 | denotes | endoplasmic reticulum |
| _T25 | 234-255 | FMAID:162308 | denotes | endoplasmic reticulum |
| _T26 | 234-255 | FMAID:63842 | denotes | endoplasmic reticulum |
| _T27 | 234-255 | FMAID:165250 | denotes | endoplasmic reticulum |
| _T28 | 234-255 | FMAID:199093 | denotes | endoplasmic reticulum |
| _T29 | 234-255 | FMAID:165026 | denotes | endoplasmic reticulum |
| _T30 | 234-255 | FMAID:66897 | denotes | endoplasmic reticulum |
| _T31 | 234-255 | FMAID:188464 | denotes | endoplasmic reticulum |
| _T32 | 234-255 | FMAID:165141 | denotes | endoplasmic reticulum |
| _T33 | 234-255 | FMAID:80351 | denotes | endoplasmic reticulum |
| _T34 | 246-255 | FMAID:94520 | denotes | reticulum |
| _T35 | 246-255 | FMAID:7646 | denotes | reticulum |
| _T36 | 318-325 | FMAID:256055 | denotes | cytosol |
| _T37 | 537-552 | FMAID:82742 | denotes | oligosaccharide |
| _T38 | 537-552 | FMAID:196731 | denotes | oligosaccharide |
| _T39 | 739-744 | FMAID:93672 | denotes | liver |
| _T40 | 739-744 | FMAID:7197 | denotes | liver |
| _T41 | 745-755 | FMAID:165145 | denotes | microsomes |
| _T42 | 789-796 | FMAID:256055 | denotes | cytosol |
| _T43 | 850-858 | FMAID:167180 | denotes | antibody |
| _T44 | 865-869 | FMAID:217859 | denotes | rate |
| _T45 | 1016-1031 | FMAID:196731 | denotes | oligosaccharide |
| _T46 | 1016-1031 | FMAID:82742 | denotes | oligosaccharide |
| _T47 | 1103-1110 | FMAID:256055 | denotes | cytosol |
| _T48 | 1269-1279 | FMAID:165145 | denotes | microsomes |
| _T49 | 1357-1364 | FMAID:165447 | denotes | protein |
| _T50 | 1357-1364 | FMAID:67257 | denotes | protein |
uniprot-human
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 91-93 | http://www.uniprot.org/uniprot/P03372 | denotes | ER |
| T2 | 257-259 | http://www.uniprot.org/uniprot/P03372 | denotes | ER |
| T3 | 268-270 | http://www.uniprot.org/uniprot/P03372 | denotes | ER |
| T4 | 359-361 | http://www.uniprot.org/uniprot/P03372 | denotes | ER |
| T5 | 572-574 | http://www.uniprot.org/uniprot/P03372 | denotes | ER |
| T6 | 1372-1374 | http://www.uniprot.org/uniprot/P03372 | denotes | ER |
uniprot-mouse
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 91-93 | http://www.uniprot.org/uniprot/P19785 | denotes | ER |
| T2 | 257-259 | http://www.uniprot.org/uniprot/P19785 | denotes | ER |
| T3 | 268-270 | http://www.uniprot.org/uniprot/P19785 | denotes | ER |
| T4 | 359-361 | http://www.uniprot.org/uniprot/P19785 | denotes | ER |
| T5 | 572-574 | http://www.uniprot.org/uniprot/P19785 | denotes | ER |
| T6 | 1372-1374 | http://www.uniprot.org/uniprot/P19785 | denotes | ER |
GO-BP
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 56-81 | http://purl.obolibrary.org/obo/GO_0015772 | denotes | oligosaccharide transport |
| T2 | 537-562 | http://purl.obolibrary.org/obo/GO_0015772 | denotes | oligosaccharide transport |
| T3 | 1016-1041 | http://purl.obolibrary.org/obo/GO_0015772 | denotes | oligosaccharide transport |
| T4 | 72-81 | http://purl.obolibrary.org/obo/GO_0006810 | denotes | transport |
| T5 | 553-562 | http://purl.obolibrary.org/obo/GO_0006810 | denotes | transport |
| T6 | 340-351 | http://purl.obolibrary.org/obo/GO_0006810 | denotes | transporter |
| T7 | 432-443 | http://purl.obolibrary.org/obo/GO_0006810 | denotes | transporter |
| T8 | 1126-1137 | http://purl.obolibrary.org/obo/GO_0006810 | denotes | transporter |
| T9 | 1246-1257 | http://purl.obolibrary.org/obo/GO_0006810 | denotes | transported |
| T10 | 117-144 | http://purl.obolibrary.org/obo/GO_0006487 | denotes | N-glycosylation of proteins |
| T11 | 119-132 | http://purl.obolibrary.org/obo/GO_0070085 | denotes | glycosylation |
| T12 | 119-144 | http://purl.obolibrary.org/obo/GO_0006486 | denotes | glycosylation of proteins |
| T13 | 1016-1050 | http://purl.obolibrary.org/obo/GO_0015422 | denotes | oligosaccharide transport activity |
| T14 | 1016-1050 | http://purl.obolibrary.org/obo/GO_0015478 | denotes | oligosaccharide transport activity |
| T15 | 1016-1050 | http://purl.obolibrary.org/obo/GO_0015157 | denotes | oligosaccharide transport activity |
| T16 | 1032-1050 | http://purl.obolibrary.org/obo/GO_0005215 | denotes | transport activity |
| T17 | 1126-1146 | http://purl.obolibrary.org/obo/GO_0005215 | denotes | transporter activity |
| T18 | 1032-1050 | http://purl.obolibrary.org/obo/GO_0015291 | denotes | transport activity |
| T19 | 1126-1146 | http://purl.obolibrary.org/obo/GO_0015291 | denotes | transporter activity |
| T20 | 1032-1050 | http://purl.obolibrary.org/obo/GO_0051050 | denotes | transport activity |
| T21 | 1126-1146 | http://purl.obolibrary.org/obo/GO_0051050 | denotes | transporter activity |
| T22 | 1345-1364 | http://purl.obolibrary.org/obo/GO_0015031 | denotes | transporter protein |
| T23 | 1357-1383 | http://purl.obolibrary.org/obo/GO_0045048 | denotes | protein on the ER membrane |
GO-MF
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 850-858 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibody |
GO-CC
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 91-93 | http://purl.obolibrary.org/obo/GO_0005783 | denotes | ER |
| T2 | 257-259 | http://purl.obolibrary.org/obo/GO_0005783 | denotes | ER |
| T3 | 268-270 | http://purl.obolibrary.org/obo/GO_0005783 | denotes | ER |
| T4 | 359-361 | http://purl.obolibrary.org/obo/GO_0005783 | denotes | ER |
| T5 | 572-574 | http://purl.obolibrary.org/obo/GO_0005783 | denotes | ER |
| T6 | 1372-1374 | http://purl.obolibrary.org/obo/GO_0005783 | denotes | ER |
| T7 | 101-108 | http://purl.obolibrary.org/obo/GO_0005829 | denotes | cytosol |
| T8 | 318-325 | http://purl.obolibrary.org/obo/GO_0005829 | denotes | cytosol |
| T9 | 789-796 | http://purl.obolibrary.org/obo/GO_0005829 | denotes | cytosol |
| T10 | 1103-1110 | http://purl.obolibrary.org/obo/GO_0005829 | denotes | cytosol |
| T11 | 221-255 | http://purl.obolibrary.org/obo/GO_0048238 | denotes | lumen of the endoplasmic reticulum |
| T12 | 221-255 | http://purl.obolibrary.org/obo/GO_0044166 | denotes | lumen of the endoplasmic reticulum |
| T13 | 221-255 | http://purl.obolibrary.org/obo/GO_0005788 | denotes | lumen of the endoplasmic reticulum |
| T14 | 221-255 | http://purl.obolibrary.org/obo/GO_0048237 | denotes | lumen of the endoplasmic reticulum |
| T15 | 234-255 | http://purl.obolibrary.org/obo/GO_0005783 | denotes | endoplasmic reticulum |
| T16 | 359-370 | http://purl.obolibrary.org/obo/GO_0005789 | denotes | ER membrane |
| T17 | 1372-1383 | http://purl.obolibrary.org/obo/GO_0005789 | denotes | ER membrane |
| T18 | 362-370 | http://purl.obolibrary.org/obo/GO_0016020 | denotes | membrane |
| T19 | 1375-1383 | http://purl.obolibrary.org/obo/GO_0016020 | denotes | membrane |
| T20 | 572-580 | http://purl.obolibrary.org/obo/GO_0005788 | denotes | ER lumen |
UBERON-AE
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 739-744 | http://purl.obolibrary.org/obo/UBERON_0002107 | denotes | liver |
GlycoBiology-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 739-744 | http://purl.obolibrary.org/obo/MAT_0000097 | denotes | liver |
GlycoBiology-Motifs
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 631-639 | http://rdf.glycoinfo.org/glycan/G00027MO | denotes | N-glycan |
GlycoBiology-Epitope
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| PD-GlycoEpitope-B_T1 | 623-630 | id | denotes | labeled |
| PD-GlycoEpitope-B_T2 | 682-689 | id | denotes | labeled |
performance-test
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| PD-UBERON-AE-B_T1 | 739-744 | http://purl.obolibrary.org/obo/UBERON_0002107 | denotes | liver |
Anatomy-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 739-744 | Body_part | denotes | liver | http://purl.obolibrary.org/obo/MAT_0000097 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 733-738 | OrganismTaxon | denotes | mouse | 10088|10090 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 101-108 | Body_part | denotes | cytosol | http://purl.obolibrary.org/obo/GO_0005829 |
| T2 | 221-226 | Body_part | denotes | lumen | http://purl.obolibrary.org/obo/UBERON_0000464 |
| T3 | 246-255 | Body_part | denotes | reticulum | http://purl.obolibrary.org/obo/UBERON_0007361 |
| T4 | 318-325 | Body_part | denotes | cytosol | http://purl.obolibrary.org/obo/GO_0005829 |
| T5 | 362-370 | Body_part | denotes | membrane | http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158 |
| T8 | 575-580 | Body_part | denotes | lumen | http://purl.obolibrary.org/obo/UBERON_0000464 |
| T9 | 739-744 | Body_part | denotes | liver | http://purl.obolibrary.org/obo/UBERON_0002107 |
| T10 | 789-796 | Body_part | denotes | cytosol | http://purl.obolibrary.org/obo/GO_0005829 |
| T11 | 1103-1110 | Body_part | denotes | cytosol | http://purl.obolibrary.org/obo/GO_0005829 |
| T12 | 1375-1383 | Body_part | denotes | membrane | http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158 |