PubMed:19427209 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/19427209","sourcedb":"PubMed","sourceid":"19427209","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/19427209","text":"Highly selective tuning of a silkworm olfactory receptor to a key mulberry leaf volatile.\nBACKGROUND: The olfactory system plays an important role in the recognition of leaf volatiles during the search of folivore insects for a suitable plant host. For example, volatiles emitted by mulberry leaves trigger chemotaxis behavior in the silkworms Bombyx mori, and as a consequence, they preferentially reside on and consume mulberry leaves. Here, we aimed to identify natural chemoattractants and their corresponding olfactory receptors (Ors) involved in silkworm behavior to mulberry leaves.\nRESULTS: Chemotaxis behavioral assays for headspace volatiles detected by gas chromatography-mass spectroscopy analysis revealed that among the volatiles that were emitted by mulberry leaves, cis-jasmone was the most potent attractant for silkworms, working at a threshold of 30 pg from [corrected] 20 cm distance. Among a total of 66 Ors identified in the B. mori genome, we found that 23 were expressed in the olfactory organs during larval stages. Functional analysis of all the larvae-expressed Ors in Xenopus oocytes revealed that one Or, termed BmOr-56, showed a high sensitivity to cis-jasmone. In addition, the ligand-receptor activity of BmOr-56 reflected the chemotaxis behavioral response of silkworms.\nCONCLUSIONS: We identified cis-jasmone as a potent attractant in mulberry leaves for silkworms and provide evidence that a highly tuned receptor, BmOr-56, may mediate this behavioral attraction. The current study sheds light on the mechanism of the correlation between olfactory perception in folivore insects and chemotaxis behavior to a natural volatile emitted by green leaves.","tracks":[{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":29,"end":37},"obj":"Species:7091"},{"id":"T2","span":{"begin":334,"end":343},"obj":"Species:7091"},{"id":"T3","span":{"begin":344,"end":355},"obj":"Species:7091"},{"id":"T4","span":{"begin":552,"end":560},"obj":"Species:7091"},{"id":"T5","span":{"begin":782,"end":793},"obj":"Chemical:MESH:C086299"},{"id":"T6","span":{"begin":829,"end":838},"obj":"Species:7091"},{"id":"T7","span":{"begin":947,"end":954},"obj":"Species:7091"},{"id":"T8","span":{"begin":1096,"end":1103},"obj":"Species:8355"},{"id":"T9","span":{"begin":1141,"end":1148},"obj":"Gene:100379311"},{"id":"T10","span":{"begin":1179,"end":1190},"obj":"Chemical:MESH:C086299"},{"id":"T11","span":{"begin":1237,"end":1244},"obj":"Gene:100379311"},{"id":"T12","span":{"begin":1293,"end":1302},"obj":"Species:7091"},{"id":"T13","span":{"begin":1331,"end":1342},"obj":"Chemical:MESH:C086299"},{"id":"T14","span":{"begin":1389,"end":1398},"obj":"Species:7091"},{"id":"T15","span":{"begin":1450,"end":1457},"obj":"Gene:100379311"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"},{"subj":"T9","pred":"source","obj":"silkworm"},{"subj":"T10","pred":"source","obj":"silkworm"},{"subj":"T11","pred":"source","obj":"silkworm"},{"subj":"T12","pred":"source","obj":"silkworm"},{"subj":"T13","pred":"source","obj":"silkworm"},{"subj":"T14","pred":"source","obj":"silkworm"},{"subj":"T15","pred":"source","obj":"silkworm"}]},{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":29,"end":37},"obj":"Species:7091"},{"id":"T10","span":{"begin":1179,"end":1190},"obj":"Chemical:MESH:C086299"},{"id":"T11","span":{"begin":1237,"end":1244},"obj":"Gene:100379311"},{"id":"T12","span":{"begin":1293,"end":1302},"obj":"Species:7091"},{"id":"T13","span":{"begin":1331,"end":1342},"obj":"Chemical:MESH:C086299"},{"id":"T14","span":{"begin":1389,"end":1398},"obj":"Species:7091"},{"id":"T15","span":{"begin":1450,"end":1457},"obj":"Gene:100379311"},{"id":"T2","span":{"begin":334,"end":343},"obj":"Species:7091"},{"id":"T3","span":{"begin":344,"end":355},"obj":"Species:7091"},{"id":"T4","span":{"begin":552,"end":560},"obj":"Species:7091"},{"id":"T5","span":{"begin":782,"end":793},"obj":"Chemical:MESH:C086299"},{"id":"T6","span":{"begin":829,"end":838},"obj":"Species:7091"},{"id":"T7","span":{"begin":947,"end":954},"obj":"Species:7091"},{"id":"T8","span":{"begin":1096,"end":1103},"obj":"Species:8355"},{"id":"T9","span":{"begin":1141,"end":1148},"obj":"Gene:100379311"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkwormbase"},{"subj":"T10","pred":"source","obj":"silkwormbase"},{"subj":"T11","pred":"source","obj":"silkwormbase"},{"subj":"T12","pred":"source","obj":"silkwormbase"},{"subj":"T13","pred":"source","obj":"silkwormbase"},{"subj":"T14","pred":"source","obj":"silkwormbase"},{"subj":"T15","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"},{"subj":"T9","pred":"source","obj":"silkwormbase"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"silkworm","color":"#b3ec93","default":true},{"id":"silkwormbase","color":"#ec93cd"}]}]}}