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PubMed:19252170 JSONTXT

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PMID_GLOBAL

Id Subject Object Predicate Lexical cue mondo_id
T1 342-361 DiseaseOrPhenotypicFeature denotes embryonal carcinoma 0003581|0005440|0018843

GlyCosmos15-Glycan

Id Subject Object Predicate Lexical cue image
T1 800-806 Glycan denotes SSEA-4 https://api.glycosmos.org/wurcs2image/latest/png/binary/G84263XI

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 342-361 Disease denotes embryonal carcinoma http://purl.obolibrary.org/obo/MONDO_0005440

Glycan-GlyCosmos

Id Subject Object Predicate Lexical cue image
T1 800-806 Glycan denotes SSEA-4 https://api.glycosmos.org/wurcs2image/latest/png/binary/G84263XI

GlyCosmos-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 800-806 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes SSEA-4 http://www.glycoepitope.jp/epitopes/EP0044

GlyCosmos15-HP

Id Subject Object Predicate Lexical cue hp_id
T1 352-361 Phenotype denotes carcinoma HP:0030731

GlyCosmos15-CL

Id Subject Object Predicate Lexical cue cl_id
T1 23-39 Cell denotes epithelial cells http://purl.obolibrary.org/obo/CL:0000066
T2 266-282 Cell denotes epithelial cells http://purl.obolibrary.org/obo/CL:0000066

GlyCosmos15-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 23-39 Body_part denotes epithelial cells http://purl.obolibrary.org/obo/CL_0000066
T2 266-282 Body_part denotes epithelial cells http://purl.obolibrary.org/obo/CL_0000066
T3 970-975 Body_part denotes spots http://purl.obolibrary.org/obo/UBERON_2002283
T4 1024-1029 Body_part denotes spots http://purl.obolibrary.org/obo/UBERON_2002283

GlyCosmos15-MONDO

Id Subject Object Predicate Lexical cue mondo_id
T1 342-361 Disease denotes embryonal carcinoma MONDO:0003581|MONDO:0005440|MONDO:0018843

GlyCosmos15-Sentences

Id Subject Object Predicate Lexical cue
T1 0-74 Sentence denotes Proteomics analysis of epithelial cells reprogrammed in cell-free extract.
T2 75-220 Sentence denotes The functional reprogramming of a differentiated cell to a pluripotent state presents potential beneficial applications in regenerative medicine.
T3 221-388 Sentence denotes We report here the proteomic profile of 293T epithelial cells reprogrammed to a pluripotent state using undifferentiated embryonal carcinoma (NCCIT) cellular extracts.
T4 389-570 Sentence denotes 293T cells were reversibly permeabilized with streptolysin O, incubated in an extract of NCCIT cells or a control extract of 293T cells for 1 h, resealed with CaCl(2), and cultured.
T5 571-735 Sentence denotes OCT4 and SOX2 gene expression were up-regulated in NCCIT extract-treated cells relative to control cells, whereas there was no alteration in DNMT3B gene expression.
T6 736-913 Sentence denotes Thirty percent of NCCIT extract-treated cells were positive for SSEA-4, and karyotyping confirmed their 293T origin, excluding the possibility of contamination from NCCIT cells.
T7 914-1003 Sentence denotes Two-dimensional PAGE revealed approximately 400 protein spots for each cell type studied.
T8 1004-1168 Sentence denotes At least 10 protein spots in the proteome of NCCIT extract-treated cells had an expression profile similar to that of NCCIT and remained unaltered in control cells.
T9 1169-1318 Sentence denotes Using tandem mass spectrometry, we identified these proteins, which include 78-kDa glucose-regulated protein precursor and tropomyosin alpha-3 chain.
T10 1319-1444 Sentence denotes This investigation provides the first evidence that proteins are altered in a specific manner in NCCIT extract-treated cells.
T11 1445-1541 Sentence denotes This is the first report on the proteomic characterization of the nuclear reprogramming process.

GlyCosmos15-GlycoEpitope

Id Subject Object Predicate Lexical cue glycoepitope_id
T1 800-806 http://purl.jp/bio/12/glyco/glycan#Glycan_epitope denotes SSEA-4 http://www.glycoepitope.jp/epitopes/EP0044

GlyCosmos15-FMA

Id Subject Object Predicate Lexical cue db_id
T1 23-39 Body_part denotes epithelial cells FMA:66768
T2 266-282 Body_part denotes epithelial cells FMA:66768

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 23-39 Body_part denotes epithelial cells http://purl.obolibrary.org/obo/CL_0000066
T2 266-282 Body_part denotes epithelial cells http://purl.obolibrary.org/obo/CL_0000066
T3 970-975 Body_part denotes spots http://purl.obolibrary.org/obo/UBERON_2002283
T4 1024-1029 Body_part denotes spots http://purl.obolibrary.org/obo/UBERON_2002283

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 352-361 Phenotype denotes carcinoma HP:0030731

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 23-39 Cell denotes epithelial cells http://purl.obolibrary.org/obo/CL:0000066
T2 266-282 Cell denotes epithelial cells http://purl.obolibrary.org/obo/CL:0000066