PubMed:19249804 JSONTXT

{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":15,"end":47},"obj":"Species:271108"},{"id":"T2","span":{"begin":48,"end":53},"obj":"Gene:1488672"},{"id":"T3","span":{"begin":99,"end":131},"obj":"Species:271108"},{"id":"T4","span":{"begin":133,"end":138},"obj":"Species:271108"},{"id":"T5","span":{"begin":140,"end":145},"obj":"Gene:1488672"},{"id":"T6","span":{"begin":277,"end":282},"obj":"Species:271108"},{"id":"T7","span":{"begin":388,"end":392},"obj":"Chemical:-"},{"id":"T8","span":{"begin":803,"end":807},"obj":"Chemical:-"},{"id":"T9","span":{"begin":998,"end":1003},"obj":"Species:271108"}],"text":"Involvement of Bombyx mori nucleopolyhedrovirus ORF41 (Bm41) in BV production and ODV envelopment.\nBombyx mori nucleopolyhedrovirus (BmNPV) ORF41 (Bm41), homologous to Ac52, is a gene present in most lepidopteran nucleopolyhedroviruses. Bm41 transcripts and encoded protein in BmNPV-infected cells can be detected from 3 and 6 h post-infection, respectively. Immunoassays have shown that Bm41 is not a viral structural protein and is detected in both the nuclei and cytoplasm of infected cells. A Bm41-disrupted virus (vBm(De)) and a repaired virus (vBm(Re)) were generated to investigate the function of Bm41. The results showed that Bm41 was essential for viral replication, and the disruption of Bm41 resulted in a much lower viral titer. Transmission electron microscopy revealed that disruption of Bm41 affected normal nucleocapsid envelopment and polyhedra formation in the nucleus. The disruption of Bm41 might severely affect odv-ec27 and polyhedrin expression. The disrupted virus reduced BmNPV infectivity in an LD(50) bioassay and took 18-23 h longer to kill larvae than wild-type virus in an LT(50) bioassay."}

{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":15,"end":47},"obj":"Species:271108"},{"id":"T2","span":{"begin":48,"end":53},"obj":"Gene:1488672"},{"id":"T3","span":{"begin":99,"end":131},"obj":"Species:271108"},{"id":"T4","span":{"begin":133,"end":138},"obj":"Species:271108"},{"id":"T5","span":{"begin":140,"end":145},"obj":"Gene:1488672"},{"id":"T6","span":{"begin":277,"end":282},"obj":"Species:271108"},{"id":"T7","span":{"begin":388,"end":392},"obj":"Chemical:-"},{"id":"T8","span":{"begin":803,"end":807},"obj":"Chemical:-"},{"id":"T9","span":{"begin":998,"end":1003},"obj":"Species:271108"}],"text":"Involvement of Bombyx mori nucleopolyhedrovirus ORF41 (Bm41) in BV production and ODV envelopment.\nBombyx mori nucleopolyhedrovirus (BmNPV) ORF41 (Bm41), homologous to Ac52, is a gene present in most lepidopteran nucleopolyhedroviruses. Bm41 transcripts and encoded protein in BmNPV-infected cells can be detected from 3 and 6 h post-infection, respectively. Immunoassays have shown that Bm41 is not a viral structural protein and is detected in both the nuclei and cytoplasm of infected cells. A Bm41-disrupted virus (vBm(De)) and a repaired virus (vBm(Re)) were generated to investigate the function of Bm41. The results showed that Bm41 was essential for viral replication, and the disruption of Bm41 resulted in a much lower viral titer. Transmission electron microscopy revealed that disruption of Bm41 affected normal nucleocapsid envelopment and polyhedra formation in the nucleus. The disruption of Bm41 might severely affect odv-ec27 and polyhedrin expression. The disrupted virus reduced BmNPV infectivity in an LD(50) bioassay and took 18-23 h longer to kill larvae than wild-type virus in an LT(50) bioassay."}