PubMed:19240271 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/19240271","sourcedb":"PubMed","sourceid":"19240271","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/19240271","text":"Class IIC alpha-mannosidase AfAms1 is required for morphogenesis and cellular function in Aspergillus fumigatus.\nThe mammalian ER/cytosolic alpha-mannosidase (Man2C1p), yeast vacuolar alpha-mannosidase (Ams1p) and the Aspergillus nidulans alpha-mannosidase are members of Class IIC subgroup, which is involved in oligosaccharide catabolism and N-glycan processing. Unlike their mammalian counterparts, the yeast Ams1p and A. nidulans Class IIC alpha-mannosidase are not essential for morphogenesis and cellular function. In this study, the Afams1, a gene encoding a member of Class IIC alpha-mannosidases, was identified in the opportunistic pathogen Aspergillus fumigatus. Deletion of the Afams1 led to a severe defect in conidial formation, especially at a higher temperature. In addition, abnormalities of polarity and septation were associated with the DeltaAfams1 mutant. Our results showed that the Afams1 gene, in contrast to its homolog in yeast or A. nidulans, was required for morphogenesis and cellular function in A. fumigatus.","tracks":[{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":127,"end":129},"obj":"http://www.uniprot.org/uniprot/P03372"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-human"}]},{"project":"GlycoBiology-GDGDB","denotations":[{"id":"_T1","span":{"begin":10,"end":27},"obj":"http://acgg.asia/db/diseases/gdgdb?con_ui=CON00008"},{"id":"_T2","span":{"begin":140,"end":157},"obj":"http://acgg.asia/db/diseases/gdgdb?con_ui=CON00008"},{"id":"_T3","span":{"begin":184,"end":201},"obj":"http://acgg.asia/db/diseases/gdgdb?con_ui=CON00008"},{"id":"_T4","span":{"begin":239,"end":256},"obj":"http://acgg.asia/db/diseases/gdgdb?con_ui=CON00008"},{"id":"_T5","span":{"begin":444,"end":461},"obj":"http://acgg.asia/db/diseases/gdgdb?con_ui=CON00008"},{"id":"_T6","span":{"begin":586,"end":604},"obj":"http://acgg.asia/db/diseases/gdgdb?con_ui=CON00008"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-GDGDB"},{"subj":"_T2","pred":"source","obj":"GlycoBiology-GDGDB"},{"subj":"_T3","pred":"source","obj":"GlycoBiology-GDGDB"},{"subj":"_T4","pred":"source","obj":"GlycoBiology-GDGDB"},{"subj":"_T5","pred":"source","obj":"GlycoBiology-GDGDB"},{"subj":"_T6","pred":"source","obj":"GlycoBiology-GDGDB"}]},{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":313,"end":328},"obj":"FMAID:196731"},{"id":"_T2","span":{"begin":313,"end":328},"obj":"FMAID:82742"},{"id":"_T3","span":{"begin":550,"end":554},"obj":"FMAID:198663"},{"id":"_T4","span":{"begin":912,"end":916},"obj":"FMAID:198663"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T2","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T3","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T4","pred":"source","obj":"GlycoBiology-FMA"}]},{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":127,"end":129},"obj":"http://www.uniprot.org/uniprot/P19785"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-mouse"}]},{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":69,"end":86},"obj":"http://purl.bioontology.org/ontology/STY/T043"},{"id":"T2","span":{"begin":90,"end":101},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/40382"},{"id":"T3","span":{"begin":90,"end":101},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/454240"},{"id":"T4","span":{"begin":90,"end":101},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/41750"},{"id":"T5","span":{"begin":90,"end":111},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/340414"},{"id":"T6","span":{"begin":90,"end":111},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/607716"},{"id":"T7","span":{"begin":90,"end":111},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/286432"},{"id":"T8","span":{"begin":90,"end":111},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/340412"},{"id":"T9","span":{"begin":218,"end":229},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/41750"},{"id":"T10","span":{"begin":218,"end":229},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/40382"},{"id":"T11","span":{"begin":218,"end":229},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/454240"},{"id":"T12","span":{"begin":218,"end":238},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/162425"},{"id":"T13","span":{"begin":502,"end":519},"obj":"http://purl.bioontology.org/ontology/STY/T043"},{"id":"T14","span":{"begin":651,"end":662},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/40382"},{"id":"T15","span":{"begin":651,"end":662},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/41750"},{"id":"T16","span":{"begin":651,"end":662},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/454240"},{"id":"T17","span":{"begin":651,"end":672},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/286432"},{"id":"T18","span":{"begin":651,"end":672},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/340414"},{"id":"T19","span":{"begin":651,"end":672},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/340412"},{"id":"T20","span":{"begin":651,"end":672},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/607716"},{"id":"T21","span":{"begin":1005,"end":1022},"obj":"http://purl.bioontology.org/ontology/STY/T043"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T2","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T3","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T4","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T5","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T6","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T7","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T8","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T9","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T10","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T11","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T12","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T13","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T14","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T15","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T16","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T17","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T18","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T19","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T20","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T21","pred":"source","obj":"GlycoBiology-NCBITAXON"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":112},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":113,"end":364},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":365,"end":520},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":521,"end":673},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":674,"end":778},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":779,"end":876},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":877,"end":1039},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":112},"obj":"Sentence"},{"id":"T2","span":{"begin":113,"end":364},"obj":"Sentence"},{"id":"T3","span":{"begin":365,"end":520},"obj":"Sentence"},{"id":"T4","span":{"begin":521,"end":673},"obj":"Sentence"},{"id":"T5","span":{"begin":674,"end":778},"obj":"Sentence"},{"id":"T6","span":{"begin":779,"end":876},"obj":"Sentence"},{"id":"T7","span":{"begin":877,"end":1039},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":112},"obj":"Sentence"},{"id":"T2","span":{"begin":113,"end":364},"obj":"Sentence"},{"id":"T3","span":{"begin":365,"end":520},"obj":"Sentence"},{"id":"T4","span":{"begin":521,"end":673},"obj":"Sentence"},{"id":"T5","span":{"begin":674,"end":778},"obj":"Sentence"},{"id":"T6","span":{"begin":779,"end":876},"obj":"Sentence"},{"id":"T7","span":{"begin":877,"end":1039},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"}]},{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":51,"end":64},"obj":"http://purl.obolibrary.org/obo/GO_0009653"},{"id":"T2","span":{"begin":484,"end":497},"obj":"http://purl.obolibrary.org/obo/GO_0009653"},{"id":"T3","span":{"begin":987,"end":1000},"obj":"http://purl.obolibrary.org/obo/GO_0009653"},{"id":"T4","span":{"begin":51,"end":77},"obj":"http://purl.obolibrary.org/obo/GO_0000902"},{"id":"T5","span":{"begin":484,"end":510},"obj":"http://purl.obolibrary.org/obo/GO_0000902"},{"id":"T6","span":{"begin":987,"end":1013},"obj":"http://purl.obolibrary.org/obo/GO_0000902"},{"id":"T7","span":{"begin":69,"end":77},"obj":"http://purl.obolibrary.org/obo/GO_0007349"},{"id":"T8","span":{"begin":502,"end":510},"obj":"http://purl.obolibrary.org/obo/GO_0007349"},{"id":"T9","span":{"begin":1005,"end":1013},"obj":"http://purl.obolibrary.org/obo/GO_0007349"},{"id":"T10","span":{"begin":313,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0009313"},{"id":"T11","span":{"begin":329,"end":339},"obj":"http://purl.obolibrary.org/obo/GO_0009056"},{"id":"T12","span":{"begin":329,"end":352},"obj":"http://purl.obolibrary.org/obo/GO_0006491"},{"id":"T13","span":{"begin":344,"end":363},"obj":"http://purl.obolibrary.org/obo/GO_0016256"},{"id":"T14","span":{"begin":344,"end":363},"obj":"http://purl.obolibrary.org/obo/GO_0006491"},{"id":"T15","span":{"begin":732,"end":741},"obj":"http://purl.obolibrary.org/obo/GO_0009058"},{"id":"T16","span":{"begin":822,"end":831},"obj":"http://purl.obolibrary.org/obo/GO_0000917"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-BP"},{"subj":"T2","pred":"source","obj":"GO-BP"},{"subj":"T3","pred":"source","obj":"GO-BP"},{"subj":"T4","pred":"source","obj":"GO-BP"},{"subj":"T5","pred":"source","obj":"GO-BP"},{"subj":"T6","pred":"source","obj":"GO-BP"},{"subj":"T7","pred":"source","obj":"GO-BP"},{"subj":"T8","pred":"source","obj":"GO-BP"},{"subj":"T9","pred":"source","obj":"GO-BP"},{"subj":"T10","pred":"source","obj":"GO-BP"},{"subj":"T11","pred":"source","obj":"GO-BP"},{"subj":"T12","pred":"source","obj":"GO-BP"},{"subj":"T13","pred":"source","obj":"GO-BP"},{"subj":"T14","pred":"source","obj":"GO-BP"},{"subj":"T15","pred":"source","obj":"GO-BP"},{"subj":"T16","pred":"source","obj":"GO-BP"}]},{"project":"GO-CC","denotations":[{"id":"T1","span":{"begin":127,"end":129},"obj":"http://purl.obolibrary.org/obo/GO_0005783"},{"id":"T2","span":{"begin":130,"end":139},"obj":"http://purl.obolibrary.org/obo/GO_0005829"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-CC"},{"subj":"T2","pred":"source","obj":"GO-CC"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":90,"end":111},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":218,"end":238},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":651,"end":672},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"746128"},{"id":"A2","pred":"db_id","subj":"T2","obj":"162425"},{"id":"A3","pred":"db_id","subj":"T3","obj":"746128"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"},{"subj":"T3","pred":"source","obj":"NCBITAXON"}]},{"project":"GlycoBiology-Motifs","denotations":[{"id":"T1","span":{"begin":344,"end":352},"obj":"http://rdf.glycoinfo.org/glycan/G00027MO"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlycoBiology-Motifs"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"uniprot-human","color":"#ecb993","default":true},{"id":"GlycoBiology-GDGDB","color":"#9f93ec"},{"id":"GlycoBiology-FMA","color":"#a1ec93"},{"id":"uniprot-mouse","color":"#ec93bb"},{"id":"GlycoBiology-NCBITAXON","color":"#93d5ec"},{"id":"sentences","color":"#ece993"},{"id":"GO-BP","color":"#cf93ec"},{"id":"GO-CC","color":"#93ecb5"},{"id":"NCBITAXON","color":"#ec9b93"},{"id":"GlycoBiology-Motifs","color":"#93a4ec"}]}]}}