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sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-83 Sentence denotes Glycosidase-induced fusion of isoprenoid gentiobiosyl lipid membranes at acidic pH.
TextSentencer_T2 84-353 Sentence denotes A difficulty in explaining the mechanism whereby archaeal lipid membrane vesicles (archaeosomes) deliver entrapped protein antigens to the MHC class I cytosolic pathway from phagolysosomes of antigen-presenting cells has been the observation that they tend not to fuse.
TextSentencer_T3 354-549 Sentence denotes Here, we determine that archaeosomes, composed of archaeal isoprenoid mixtures of glyco and phospholipids, can be highly fusogenic when exposed to the pH and enzymes found in late phagolysosomes.
TextSentencer_T4 550-642 Sentence denotes Fusions were strictly dependent on acidic pH and the presence of alpha- or beta-glucosidase.
TextSentencer_T5 643-794 Sentence denotes Resonance energy transfer (RET) assays demonstrated that fusion conditions induced lipid mixing of archaeosome lipids with self-unlabeled archaeosomes.
TextSentencer_T6 795-1074 Sentence denotes Because PC/PG/cholesterol liposomes by themselves did not fuse, it was possible to unequivocally show a fusion of rhodamine-labeled liposomes with archaeosomes by fluorescence microscopy and to demonstrate lipid mixing between labeled liposomes and archaeosomes by the RET assay.
TextSentencer_T7 1075-1227 Sentence denotes Radiotracer and (1)H NMR studies revealed that glycolipids in fused archaeosomes were not degraded significantly by glucosidase treatment during fusion.
TextSentencer_T8 1228-1565 Sentence denotes Rather, the glucosidases dramatically induced small archaeosomes to rapidly and visually aggregate at pH 4.8, but not 6.8, thus bringing membranes together appropriately as a first step in the fusion process. (1)H NMR was used to demonstrate that conditions causing aggregation correlated with binding of glucosidase to the archaeosomes.
TextSentencer_T9 1566-1763 Sentence denotes Binding at acidic pH occurred by the electrostatic interaction of positively charged glucosidase with the anionic phospholipids, although the interaction also occurred with the gentiobiosyl lipids.
TextSentencer_T10 1764-2047 Sentence denotes The data indicate a mechanism of membrane-membrane fusion for archaeal glycolipid membranes induced by glycosidase and illustrate the importance for inclusion of glycolipids in compositions of vesicles designed to deliver protein antigens to the cytosol for MHC class I presentation.
T1 0-83 Sentence denotes Glycosidase-induced fusion of isoprenoid gentiobiosyl lipid membranes at acidic pH.
T2 84-353 Sentence denotes A difficulty in explaining the mechanism whereby archaeal lipid membrane vesicles (archaeosomes) deliver entrapped protein antigens to the MHC class I cytosolic pathway from phagolysosomes of antigen-presenting cells has been the observation that they tend not to fuse.
T3 354-549 Sentence denotes Here, we determine that archaeosomes, composed of archaeal isoprenoid mixtures of glyco and phospholipids, can be highly fusogenic when exposed to the pH and enzymes found in late phagolysosomes.
T4 550-642 Sentence denotes Fusions were strictly dependent on acidic pH and the presence of alpha- or beta-glucosidase.
T5 643-794 Sentence denotes Resonance energy transfer (RET) assays demonstrated that fusion conditions induced lipid mixing of archaeosome lipids with self-unlabeled archaeosomes.
T6 795-1074 Sentence denotes Because PC/PG/cholesterol liposomes by themselves did not fuse, it was possible to unequivocally show a fusion of rhodamine-labeled liposomes with archaeosomes by fluorescence microscopy and to demonstrate lipid mixing between labeled liposomes and archaeosomes by the RET assay.
T7 1075-1227 Sentence denotes Radiotracer and (1)H NMR studies revealed that glycolipids in fused archaeosomes were not degraded significantly by glucosidase treatment during fusion.
T8 1228-1565 Sentence denotes Rather, the glucosidases dramatically induced small archaeosomes to rapidly and visually aggregate at pH 4.8, but not 6.8, thus bringing membranes together appropriately as a first step in the fusion process. (1)H NMR was used to demonstrate that conditions causing aggregation correlated with binding of glucosidase to the archaeosomes.
T9 1566-1763 Sentence denotes Binding at acidic pH occurred by the electrostatic interaction of positively charged glucosidase with the anionic phospholipids, although the interaction also occurred with the gentiobiosyl lipids.
T10 1764-2047 Sentence denotes The data indicate a mechanism of membrane-membrane fusion for archaeal glycolipid membranes induced by glycosidase and illustrate the importance for inclusion of glycolipids in compositions of vesicles designed to deliver protein antigens to the cytosol for MHC class I presentation.
T1 0-83 Sentence denotes Glycosidase-induced fusion of isoprenoid gentiobiosyl lipid membranes at acidic pH.
T2 84-353 Sentence denotes A difficulty in explaining the mechanism whereby archaeal lipid membrane vesicles (archaeosomes) deliver entrapped protein antigens to the MHC class I cytosolic pathway from phagolysosomes of antigen-presenting cells has been the observation that they tend not to fuse.
T3 354-549 Sentence denotes Here, we determine that archaeosomes, composed of archaeal isoprenoid mixtures of glyco and phospholipids, can be highly fusogenic when exposed to the pH and enzymes found in late phagolysosomes.
T4 550-642 Sentence denotes Fusions were strictly dependent on acidic pH and the presence of alpha- or beta-glucosidase.
T5 643-794 Sentence denotes Resonance energy transfer (RET) assays demonstrated that fusion conditions induced lipid mixing of archaeosome lipids with self-unlabeled archaeosomes.
T6 795-1074 Sentence denotes Because PC/PG/cholesterol liposomes by themselves did not fuse, it was possible to unequivocally show a fusion of rhodamine-labeled liposomes with archaeosomes by fluorescence microscopy and to demonstrate lipid mixing between labeled liposomes and archaeosomes by the RET assay.
T7 1075-1227 Sentence denotes Radiotracer and (1)H NMR studies revealed that glycolipids in fused archaeosomes were not degraded significantly by glucosidase treatment during fusion.
T8 1228-1565 Sentence denotes Rather, the glucosidases dramatically induced small archaeosomes to rapidly and visually aggregate at pH 4.8, but not 6.8, thus bringing membranes together appropriately as a first step in the fusion process. (1)H NMR was used to demonstrate that conditions causing aggregation correlated with binding of glucosidase to the archaeosomes.
T9 1566-1763 Sentence denotes Binding at acidic pH occurred by the electrostatic interaction of positively charged glucosidase with the anionic phospholipids, although the interaction also occurred with the gentiobiosyl lipids.
T10 1764-2047 Sentence denotes The data indicate a mechanism of membrane-membrane fusion for archaeal glycolipid membranes induced by glycosidase and illustrate the importance for inclusion of glycolipids in compositions of vesicles designed to deliver protein antigens to the cytosol for MHC class I presentation.

GlycoBiology-FMA

Id Subject Object Predicate Lexical cue
_T1 54-59 FMAID:165448 denotes lipid
_T2 54-59 FMAID:67264 denotes lipid
_T3 60-69 FMAID:7145 denotes membranes
_T4 60-69 FMAID:167608 denotes membranes
_T5 60-69 FMAID:30322 denotes membranes
_T6 60-69 FMAID:93573 denotes membranes
_T7 142-147 FMAID:165448 denotes lipid
_T8 142-147 FMAID:67264 denotes lipid
_T9 199-206 FMAID:165447 denotes protein
_T10 199-206 FMAID:67257 denotes protein
_T11 223-226 FMAID:198016 denotes MHC
_T12 258-272 FMAID:83022 denotes phagolysosomes
_T13 258-272 FMAID:90000 denotes phagolysosomes
_T14 276-300 FMAID:273572 denotes antigen-presenting cells
_T15 276-300 FMAID:273564 denotes antigen-presenting cells
_T16 276-300 FMAID:273565 denotes antigen-presenting cells
_T17 276-300 FMAID:273566 denotes antigen-presenting cells
_T18 295-300 FMAID:68646 denotes cells
_T19 295-300 FMAID:169002 denotes cells
_T20 446-459 FMAID:82779 denotes phospholipids
_T21 446-459 FMAID:196772 denotes phospholipids
_T22 534-548 FMAID:90000 denotes phagolysosomes
_T23 534-548 FMAID:83022 denotes phagolysosomes
_T24 726-731 FMAID:165448 denotes lipid
_T25 726-731 FMAID:67264 denotes lipid
_T26 754-760 FMAID:165448 denotes lipids
_T27 754-760 FMAID:67264 denotes lipids
_T28 1001-1006 FMAID:165448 denotes lipid
_T29 1001-1006 FMAID:67264 denotes lipid
_T30 1122-1133 FMAID:82780 denotes glycolipids
_T31 1122-1133 FMAID:196773 denotes glycolipids
_T32 1365-1374 FMAID:167608 denotes membranes
_T33 1365-1374 FMAID:30322 denotes membranes
_T34 1365-1374 FMAID:93573 denotes membranes
_T35 1365-1374 FMAID:7145 denotes membranes
_T36 1680-1693 FMAID:82779 denotes phospholipids
_T37 1680-1693 FMAID:196772 denotes phospholipids
_T38 1756-1762 FMAID:165448 denotes lipids
_T39 1756-1762 FMAID:67264 denotes lipids
_T40 1784-1814 FMAID:155535 denotes mechanism of membrane-membrane
_T41 1784-1814 FMAID:56844 denotes mechanism of membrane-membrane
_T42 1784-1814 FMAID:161386 denotes mechanism of membrane-membrane
_T43 1794-1814 FMAID:185717 denotes of membrane-membrane
_T44 1794-1814 FMAID:118424 denotes of membrane-membrane
_T45 1794-1814 FMAID:25709 denotes of membrane-membrane
_T46 1794-1814 FMAID:56723 denotes of membrane-membrane
_T47 1794-1814 FMAID:155261 denotes of membrane-membrane
_T48 1794-1814 FMAID:155238 denotes of membrane-membrane
_T49 1794-1814 FMAID:56720 denotes of membrane-membrane
_T50 1794-1814 FMAID:56732 denotes of membrane-membrane
_T51 1794-1814 FMAID:155235 denotes of membrane-membrane
_T52 1797-1814 FMAID:199959 denotes membrane-membrane
_T53 1797-1814 FMAID:196357 denotes membrane-membrane
_T54 1797-1814 FMAID:82500 denotes membrane-membrane
_T55 1797-1814 FMAID:93573 denotes membrane-membrane
_T56 1797-1814 FMAID:7145 denotes membrane-membrane
_T57 1797-1814 FMAID:158316 denotes membrane-membrane
_T58 1797-1814 FMAID:56428 denotes membrane-membrane
_T59 1797-1814 FMAID:215544 denotes membrane-membrane
_T60 1797-1814 FMAID:19674 denotes membrane-membrane
_T61 1797-1814 FMAID:112046 denotes membrane-membrane
_T62 1797-1814 FMAID:76711 denotes membrane-membrane
_T63 1797-1814 FMAID:183865 denotes membrane-membrane
_T64 1797-1814 FMAID:158819 denotes membrane-membrane
_T65 1797-1814 FMAID:154308 denotes membrane-membrane
_T66 1797-1814 FMAID:210647 denotes membrane-membrane
_T67 1797-1814 FMAID:219099 denotes membrane-membrane
_T68 1797-1814 FMAID:216391 denotes membrane-membrane
_T69 1797-1814 FMAID:261266 denotes membrane-membrane
_T70 1797-1814 FMAID:167424 denotes membrane-membrane
_T71 1797-1814 FMAID:162357 denotes membrane-membrane
_T72 1797-1814 FMAID:63888 denotes membrane-membrane
_T73 1797-1814 FMAID:9595 denotes membrane-membrane
_T74 1797-1814 FMAID:97471 denotes membrane-membrane
_T75 1797-1814 FMAID:187764 denotes membrane-membrane
_T76 1797-1814 FMAID:30514 denotes membrane-membrane
_T77 1797-1814 FMAID:123713 denotes membrane-membrane
_T78 1797-1814 FMAID:66762 denotes membrane-membrane
_T79 1797-1814 FMAID:164917 denotes membrane-membrane
_T80 1797-1814 FMAID:121075 denotes membrane-membrane
_T81 1797-1814 FMAID:27936 denotes membrane-membrane
_T82 1797-1814 FMAID:210752 denotes membrane-membrane
_T83 1797-1814 FMAID:185647 denotes membrane-membrane
_T84 1797-1814 FMAID:199178 denotes membrane-membrane
_T85 1797-1814 FMAID:233914 denotes membrane-membrane
_T86 1797-1814 FMAID:63841 denotes membrane-membrane
_T87 1797-1814 FMAID:162306 denotes membrane-membrane
_T88 1797-1814 FMAID:162305 denotes membrane-membrane
_T89 1797-1814 FMAID:76873 denotes membrane-membrane
_T90 1797-1814 FMAID:184066 denotes membrane-membrane
_T91 1797-1814 FMAID:60959 denotes membrane-membrane
_T92 1797-1814 FMAID:56427 denotes membrane-membrane
_T93 1797-1814 FMAID:154307 denotes membrane-membrane
_T94 1797-1814 FMAID:180652 denotes membrane-membrane
_T95 1797-1814 FMAID:9581 denotes membrane-membrane
_T96 1797-1814 FMAID:97450 denotes membrane-membrane
_T97 1797-1814 FMAID:161384 denotes membrane-membrane
_T98 1835-1845 FMAID:196773 denotes glycolipid
_T99 1835-1845 FMAID:82780 denotes glycolipid
_T100 1846-1855 FMAID:167608 denotes membranes
_T101 1846-1855 FMAID:93573 denotes membranes
_T102 1846-1855 FMAID:7145 denotes membranes
_T103 1846-1855 FMAID:30322 denotes membranes
_T104 1926-1937 FMAID:196773 denotes glycolipids
_T105 1926-1937 FMAID:82780 denotes glycolipids
_T106 1941-1953 FMAID:146309 denotes compositions
_T107 1941-1953 FMAID:50603 denotes compositions
_T108 1986-1993 FMAID:165447 denotes protein
_T109 1986-1993 FMAID:67257 denotes protein
_T110 2010-2017 FMAID:256055 denotes cytosol
_T111 2022-2025 FMAID:198016 denotes MHC

uniprot-human

Id Subject Object Predicate Lexical cue
T1 670-673 http://www.uniprot.org/uniprot/P07949 denotes RET
T2 1064-1067 http://www.uniprot.org/uniprot/P07949 denotes RET
T3 803-805 http://www.uniprot.org/uniprot/P11498 denotes PC
T4 803-805 http://www.uniprot.org/uniprot/O00592 denotes PC

uniprot-mouse

Id Subject Object Predicate Lexical cue
T1 803-805 http://www.uniprot.org/uniprot/Q9R0M4 denotes PC
T2 1137-1142 http://www.uniprot.org/uniprot/O35625 denotes fused

GlycoBiology-NCBITAXON

Id Subject Object Predicate Lexical cue
T1 115-124 http://purl.bioontology.org/ontology/NCBITAXON/127244 denotes mechanism
T2 295-300 http://purl.bioontology.org/ontology/STY/T025 denotes cells
T3 529-533 http://purl.bioontology.org/ontology/NCBITAXON/8186 denotes late
T4 625-629 http://purl.bioontology.org/ontology/NCBITAXON/3554 denotes beta
T5 625-629 http://purl.bioontology.org/ontology/NCBITAXON/158455 denotes beta
T6 1784-1793 http://purl.bioontology.org/ontology/NCBITAXON/127244 denotes mechanism
T7 1797-1814 http://purl.bioontology.org/ontology/NCBITAXON/95169 denotes membrane-membrane
T8 1797-1814 http://purl.bioontology.org/ontology/NCBITAXON/1232031 denotes membrane-membrane
T9 1797-1814 http://purl.bioontology.org/ontology/NCBITAXON/676144 denotes membrane-membrane

GO-BP

Id Subject Object Predicate Lexical cue
T1 276-294 http://purl.obolibrary.org/obo/GO_0019882 denotes antigen-presenting
T2 1165-1173 http://purl.obolibrary.org/obo/GO_0009056 denotes degraded
T3 1806-1821 http://purl.obolibrary.org/obo/GO_0061025 denotes membrane fusion

GO-MF

Id Subject Object Predicate Lexical cue
T1 1522-1529 http://purl.obolibrary.org/obo/GO_0070026 denotes binding
T2 1566-1573 http://purl.obolibrary.org/obo/GO_0070026 denotes Binding
T3 1522-1529 http://purl.obolibrary.org/obo/GO_0003680 denotes binding
T4 1566-1573 http://purl.obolibrary.org/obo/GO_0003680 denotes Binding
T5 1522-1529 http://purl.obolibrary.org/obo/GO_0017091 denotes binding
T6 1566-1573 http://purl.obolibrary.org/obo/GO_0017091 denotes Binding
T7 1522-1529 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T8 1566-1573 http://purl.obolibrary.org/obo/GO_0005488 denotes Binding

GO-CC

Id Subject Object Predicate Lexical cue
T1 60-69 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T2 1365-1374 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T3 148-156 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T4 1797-1805 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T5 1806-1814 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T6 148-165 http://purl.obolibrary.org/obo/GO_0012506 denotes membrane vesicles
T7 157-165 http://purl.obolibrary.org/obo/GO_0031982 denotes vesicles
T8 1957-1965 http://purl.obolibrary.org/obo/GO_0031982 denotes vesicles
T9 235-244 http://purl.obolibrary.org/obo/GO_0005829 denotes cytosolic
T10 2010-2017 http://purl.obolibrary.org/obo/GO_0005829 denotes cytosol
T11 258-272 http://purl.obolibrary.org/obo/GO_0032010 denotes phagolysosomes
T12 534-548 http://purl.obolibrary.org/obo/GO_0032010 denotes phagolysosomes
T13 295-300 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T14 806-808 http://purl.obolibrary.org/obo/GO_0010287 denotes PG

GlycoBiology-Epitope

Id Subject Object Predicate Lexical cue
PD-GlycoEpitope-B_T1 919-926 id denotes labeled
PD-GlycoEpitope-B_T2 1022-1029 id denotes labeled

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 60-69 Body_part denotes membranes http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T4 148-156 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T7 1365-1374 Body_part denotes membranes http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T10 1797-1805 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T13 1806-1814 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T16 1846-1855 Body_part denotes membranes http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T19 2010-2017 Body_part denotes cytosol http://purl.obolibrary.org/obo/GO_0005829