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PubMed:1899862 JSONTXT

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LitCoin-PubTator-for-Tuning

Id Subject Object Predicate Lexical cue tao:has_database_id tao:has_standard_notation
3 20-27 ChemicalEntity denotes lysines MESH:D008239
4 32-41 ChemicalEntity denotes arginines MESH:D001120
5 135-166 GeneOrGeneProduct denotes NADPH-cytochrome P450 reductase Gene:5447
17 262-293 GeneOrGeneProduct denotes NADPH-cytochrome P450 reductase Gene:5447
18 440-449 SequenceVariant denotes Lys94-Glu p.K94E
19 451-460 SequenceVariant denotes Lys99-Glu p.K99E
20 462-472 SequenceVariant denotes Lys105-Glu p.K105E
21 474-484 SequenceVariant denotes Lys440-Glu p.K440E
22 486-496 SequenceVariant denotes Lys453-Glu p.K453E
23 498-508 SequenceVariant denotes Arg455-Glu p.R455E
24 514-524 SequenceVariant denotes Lys463-Glu p.K463E
25 887-897 SequenceVariant denotes Arg135-Leu p.R135L
26 900-910 SequenceVariant denotes Arg136-Leu p.R136L
27 913-923 SequenceVariant denotes Arg137-Leu p.R137L

LitCoin-PubTator-for-Tuning-SeqVar

Id Subject Object Predicate Lexical cue
T1 82-87 SequenceVariant denotes P450d
T2 206-211 SequenceVariant denotes P450d
T3 213-218 SequenceVariant denotes P450d
T4 337-342 SequenceVariant denotes P450d
T5 440-449 SequenceVariant denotes Lys94-Glu
T6 451-460 SequenceVariant denotes Lys99-Glu
T7 462-472 SequenceVariant denotes Lys105-Glu
T8 474-484 SequenceVariant denotes Lys440-Glu
T9 486-496 SequenceVariant denotes Lys453-Glu
T10 498-508 SequenceVariant denotes Arg455-Glu
T11 514-524 SequenceVariant denotes Lys463-Glu
T12 887-897 SequenceVariant denotes Arg135-Leu
T13 900-910 SequenceVariant denotes Arg136-Leu
T14 913-923 SequenceVariant denotes Arg137-Leu
T15 1129-1139 SequenceVariant denotes Arg135-Arg
T16 1153-1158 SequenceVariant denotes P450d

sentences

Id Subject Object Predicate Lexical cue
T1 0-117 Sentence denotes Probing the role of lysines and arginines in the catalytic function of cytochrome P450d by site-directed mutagenesis.
T2 118-167 Sentence denotes Interaction with NADPH-cytochrome P450 reductase.
T3 168-382 Sentence denotes To identify amino acids of cytochrome P450d (P450d) which participate in the interaction with NADPH-cytochrome P450 reductase, we changed conserved ionic amino acids of P450d to others by site-directed mutagenesis.
T4 383-622 Sentence denotes Turnover numbers (0.032-0.008 min-1) of purified mutants Lys94-Glu, Lys99-Glu, Lys105-Glu, Lys440-Glu, Lys453-Glu, Arg455-Glu, and Lys463-Glu toward 7-ethoxycoumarin were much lower than that (0.380 min-1) of the wild type at 25 degrees C.
T5 623-810 Sentence denotes Reduction rates (less than 0.054 s-1) of the heme of all mutants (0.1 microM) in the presence of NADPH and the reductase (0.3 microM) were much lower than that (5.9 s-1) of the wild type.
T6 811-1024 Sentence denotes Furthermore, a turnover number (0.042 min-1) of a microsomal triple mutant (Arg135-Leu + Arg136-Leu + Arg137-Leu) of a conserved Arg cluster was much lower than that (0.674 min-1) of the wild type at 37 degrees C.
T7 1025-1389 Sentence denotes Thus, we suggest that Lys94, Lys99, Lys105, Lys440, Lys453, Arg455, Lys463, and perhaps the Arg cluster Arg135-Arg136-Arg137 of P450d will participate in the intermolecular electron transfer process by forming ionic bridges between the two proteins and/or by orienting appropriate geometry for electron transfer on the interfacial surface between the two proteins.
T1 0-117 Sentence denotes Probing the role of lysines and arginines in the catalytic function of cytochrome P450d by site-directed mutagenesis.
T2 118-167 Sentence denotes Interaction with NADPH-cytochrome P450 reductase.
T3 168-382 Sentence denotes To identify amino acids of cytochrome P450d (P450d) which participate in the interaction with NADPH-cytochrome P450 reductase, we changed conserved ionic amino acids of P450d to others by site-directed mutagenesis.
T4 383-622 Sentence denotes Turnover numbers (0.032-0.008 min-1) of purified mutants Lys94-Glu, Lys99-Glu, Lys105-Glu, Lys440-Glu, Lys453-Glu, Arg455-Glu, and Lys463-Glu toward 7-ethoxycoumarin were much lower than that (0.380 min-1) of the wild type at 25 degrees C.
T5 623-810 Sentence denotes Reduction rates (less than 0.054 s-1) of the heme of all mutants (0.1 microM) in the presence of NADPH and the reductase (0.3 microM) were much lower than that (5.9 s-1) of the wild type.
T6 811-1024 Sentence denotes Furthermore, a turnover number (0.042 min-1) of a microsomal triple mutant (Arg135-Leu + Arg136-Leu + Arg137-Leu) of a conserved Arg cluster was much lower than that (0.674 min-1) of the wild type at 37 degrees C.
T7 1025-1389 Sentence denotes Thus, we suggest that Lys94, Lys99, Lys105, Lys440, Lys453, Arg455, Lys463, and perhaps the Arg cluster Arg135-Arg136-Arg137 of P450d will participate in the intermolecular electron transfer process by forming ionic bridges between the two proteins and/or by orienting appropriate geometry for electron transfer on the interfacial surface between the two proteins.