| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-138 |
Sentence |
denotes |
Molecular staging of pathologically negative sentinel lymph nodes from melanoma patients using multimarker, quantitative real-time rt-PCR. |
| TextSentencer_T2 |
139-354 |
Sentence |
denotes |
The aim of this study was to evaluate the prognostic potential of quantitative reverse-transcription, polymerase chain reaction (qRT-PCR) in melanoma patients with pathologically negative sentinel lymph nodes (SLN). |
| TextSentencer_T3 |
355-641 |
Sentence |
denotes |
Our study included 195 node-negative melanoma patients with a Breslow thickness greater than 0.76 mm (n = 158), or less than 0.76 mm but who had Clark level IV-V, microscopic ulceration, or pathological signs of regression (n = 32), and five patients with melanoma of unknown thickness. |
| TextSentencer_T4 |
642-694 |
Sentence |
denotes |
SLNs were examined by serial-section histopathology. |
| TextSentencer_T5 |
695-815 |
Sentence |
denotes |
A portion of each SLN was frozen for qRT-PCR analysis using markers Tyrosinase, MART1, SSX2, MAGEA3, PAX3, and GalNAc-T. |
| TextSentencer_T6 |
816-929 |
Sentence |
denotes |
In addition, two other markers (PLAB and L1CAM) were evaluated for melanoma specificity but not for SLN analysis. |
| TextSentencer_T7 |
930-1013 |
Sentence |
denotes |
Median follow-up was 64 months, during which time there were 15 (7.7%) recurrences. |
| TextSentencer_T8 |
1014-1066 |
Sentence |
denotes |
A total of 370 lymph nodes were analyzed by qRT-PCR. |
| TextSentencer_T9 |
1067-1167 |
Sentence |
denotes |
No association was found between quantitative expression level of any marker and disease recurrence. |
| TextSentencer_T10 |
1168-1349 |
Sentence |
denotes |
Previously published primer designs were tested for PAX3 and GalNAc-T and revealed that alternative PAX3 transcripts are differentially expressed in melanoma and benign lymph nodes. |
| TextSentencer_T11 |
1350-1458 |
Sentence |
denotes |
No associations with recurrence were found regardless of the transcripts amplified by different primer sets. |
| TextSentencer_T12 |
1459-1587 |
Sentence |
denotes |
PLAB and L1CAM did not appear to differentiate between malignant melanoma and benign melanocytes or lymph nodes in our analysis. |
| TextSentencer_T13 |
1588-1719 |
Sentence |
denotes |
We conclude that, in this large cohort of patients, multimarker qRT-PCR analysis of SLNs did not correlate with disease recurrence. |
| TextSentencer_T14 |
1720-1851 |
Sentence |
denotes |
Our data support specific PAX3 splice variants but not GalNAc-T, PLAB or L1CAM as possible markers for melanoma metastasis to SLNs. |
| T1 |
0-138 |
Sentence |
denotes |
Molecular staging of pathologically negative sentinel lymph nodes from melanoma patients using multimarker, quantitative real-time rt-PCR. |
| T2 |
139-354 |
Sentence |
denotes |
The aim of this study was to evaluate the prognostic potential of quantitative reverse-transcription, polymerase chain reaction (qRT-PCR) in melanoma patients with pathologically negative sentinel lymph nodes (SLN). |
| T3 |
355-641 |
Sentence |
denotes |
Our study included 195 node-negative melanoma patients with a Breslow thickness greater than 0.76 mm (n = 158), or less than 0.76 mm but who had Clark level IV-V, microscopic ulceration, or pathological signs of regression (n = 32), and five patients with melanoma of unknown thickness. |
| T4 |
642-694 |
Sentence |
denotes |
SLNs were examined by serial-section histopathology. |
| T5 |
695-815 |
Sentence |
denotes |
A portion of each SLN was frozen for qRT-PCR analysis using markers Tyrosinase, MART1, SSX2, MAGEA3, PAX3, and GalNAc-T. |
| T6 |
816-929 |
Sentence |
denotes |
In addition, two other markers (PLAB and L1CAM) were evaluated for melanoma specificity but not for SLN analysis. |
| T7 |
930-1013 |
Sentence |
denotes |
Median follow-up was 64 months, during which time there were 15 (7.7%) recurrences. |
| T8 |
1014-1066 |
Sentence |
denotes |
A total of 370 lymph nodes were analyzed by qRT-PCR. |
| T9 |
1067-1167 |
Sentence |
denotes |
No association was found between quantitative expression level of any marker and disease recurrence. |
| T10 |
1168-1349 |
Sentence |
denotes |
Previously published primer designs were tested for PAX3 and GalNAc-T and revealed that alternative PAX3 transcripts are differentially expressed in melanoma and benign lymph nodes. |
| T11 |
1350-1458 |
Sentence |
denotes |
No associations with recurrence were found regardless of the transcripts amplified by different primer sets. |
| T12 |
1459-1587 |
Sentence |
denotes |
PLAB and L1CAM did not appear to differentiate between malignant melanoma and benign melanocytes or lymph nodes in our analysis. |
| T13 |
1588-1719 |
Sentence |
denotes |
We conclude that, in this large cohort of patients, multimarker qRT-PCR analysis of SLNs did not correlate with disease recurrence. |
| T14 |
1720-1851 |
Sentence |
denotes |
Our data support specific PAX3 splice variants but not GalNAc-T, PLAB or L1CAM as possible markers for melanoma metastasis to SLNs. |