PubMed:18713811
Annnotations
Glycan-Motif
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 14-25 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T2 | 237-248 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T3 | 318-329 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T4 | 716-727 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
GlyCosmos6-Glycan-Motif-Image
Id | Subject | Object | Predicate | Lexical cue | image |
---|---|---|---|---|---|
T1 | 14-25 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
T2 | 237-248 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
T3 | 318-329 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
T4 | 716-727 | Glycan_Motif | denotes | sialic acid | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY |
PMID_GLOBAL
Id | Subject | Object | Predicate | Lexical cue | mondo_id |
---|---|---|---|---|---|
T1 | 105-108 | DiseaseOrPhenotypicFeature | denotes | MAR | 0007925 |
T2 | 364-367 | DiseaseOrPhenotypicFeature | denotes | MAR | 0007925 |
T3 | 455-458 | DiseaseOrPhenotypicFeature | denotes | MAR | 0007925 |
T4 | 735-738 | DiseaseOrPhenotypicFeature | denotes | MAR | 0007925 |
T5 | 855-864 | DiseaseOrPhenotypicFeature | denotes | sensitive | 0000605 |
T6 | 994-997 | DiseaseOrPhenotypicFeature | denotes | MAR | 0007925 |
GlyCosmos6-Glycan-Motif-Structure
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 14-25 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T2 | 237-248 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T3 | 318-329 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
T4 | 716-727 | https://glytoucan.org/Structures/Glycans/G81533KY | denotes | sialic acid |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
TextSentencer_T1 | 0-38 | Sentence | denotes | The Golgi CMP-sialic acid transporter: |
TextSentencer_T2 | 39-85 | Sentence | denotes | A new CHO mutant provides functional insights. |
TextSentencer_T3 | 86-187 | Sentence | denotes | A CHO mutant line, MAR-11, was isolated using a cytotoxic lectin, Maackia amurensis agglutinin (MAA). |
TextSentencer_T4 | 188-309 | Sentence | denotes | This mutant has decreased levels of cell surface sialic acid relative to both wild-type CHO-K1 and Lec2 mutant CHO cells. |
TextSentencer_T5 | 310-441 | Sentence | denotes | The CMP-sialic acid transporter (CMP-SAT) gene in the MAR-11 mutant cell has a C-T mutation that results in a premature stop codon. |
TextSentencer_T6 | 442-605 | Sentence | denotes | As a result, MAR-11 cells express a truncated version of CMP-SAT which contains only 100 amino acids rather than the normal CMP-SAT which contains 336 amino acids. |
TextSentencer_T7 | 606-728 | Sentence | denotes | Biochemical analyses indicate that recombinant interferon-gamma (IFN-gamma) produced by the mutant cells lack sialic acid. |
TextSentencer_T8 | 729-832 | Sentence | denotes | Using MAR-11 as host cells, an EPO/IEF assay for the structure-function study of CMP-SAT was developed. |
TextSentencer_T9 | 833-938 | Sentence | denotes | This assay seems more sensitive than previous assays that were used to analyze sialylation in Lec2 cells. |
TextSentencer_T10 | 939-1136 | Sentence | denotes | Cotransfection of constructs that express CMP-SAT into MAR-11 cells completely converted the recombinant EPO to a sialylation pattern that is similar to the EPO produced by the wild-type CHO cells. |
TextSentencer_T11 | 1137-1285 | Sentence | denotes | Using this assay, we showed that CMP-SAT lacking C-terminal 18 amino acids from the cytosolic tail was able to allow high levels of EPO sialylation. |
TextSentencer_T12 | 1286-1437 | Sentence | denotes | Substitution of the Gly residues with Ile in three different transmembrane domains of CMP-SAT resulted in dramatic decreases in transporter's activity. |
TextSentencer_T13 | 1438-1649 | Sentence | denotes | The CMP-SAT only lost partial activity if the same Gly residues were substituted with Ala, suggesting that the lack of side chain in Gly residues in the transmembrane domains is essential for transport activity. |
T1 | 0-85 | Sentence | denotes | The Golgi CMP-sialic acid transporter: A new CHO mutant provides functional insights. |
T2 | 86-187 | Sentence | denotes | A CHO mutant line, MAR-11, was isolated using a cytotoxic lectin, Maackia amurensis agglutinin (MAA). |
T3 | 188-309 | Sentence | denotes | This mutant has decreased levels of cell surface sialic acid relative to both wild-type CHO-K1 and Lec2 mutant CHO cells. |
T4 | 310-441 | Sentence | denotes | The CMP-sialic acid transporter (CMP-SAT) gene in the MAR-11 mutant cell has a C-T mutation that results in a premature stop codon. |
T5 | 442-605 | Sentence | denotes | As a result, MAR-11 cells express a truncated version of CMP-SAT which contains only 100 amino acids rather than the normal CMP-SAT which contains 336 amino acids. |
T6 | 606-728 | Sentence | denotes | Biochemical analyses indicate that recombinant interferon-gamma (IFN-gamma) produced by the mutant cells lack sialic acid. |
T7 | 729-832 | Sentence | denotes | Using MAR-11 as host cells, an EPO/IEF assay for the structure-function study of CMP-SAT was developed. |
T8 | 833-938 | Sentence | denotes | This assay seems more sensitive than previous assays that were used to analyze sialylation in Lec2 cells. |
T9 | 939-1136 | Sentence | denotes | Cotransfection of constructs that express CMP-SAT into MAR-11 cells completely converted the recombinant EPO to a sialylation pattern that is similar to the EPO produced by the wild-type CHO cells. |
T10 | 1137-1285 | Sentence | denotes | Using this assay, we showed that CMP-SAT lacking C-terminal 18 amino acids from the cytosolic tail was able to allow high levels of EPO sialylation. |
T11 | 1286-1437 | Sentence | denotes | Substitution of the Gly residues with Ile in three different transmembrane domains of CMP-SAT resulted in dramatic decreases in transporter's activity. |
T12 | 1438-1649 | Sentence | denotes | The CMP-SAT only lost partial activity if the same Gly residues were substituted with Ala, suggesting that the lack of side chain in Gly residues in the transmembrane domains is essential for transport activity. |
T1 | 0-38 | Sentence | denotes | The Golgi CMP-sialic acid transporter: |
T2 | 39-85 | Sentence | denotes | A new CHO mutant provides functional insights. |
T3 | 86-187 | Sentence | denotes | A CHO mutant line, MAR-11, was isolated using a cytotoxic lectin, Maackia amurensis agglutinin (MAA). |
T4 | 188-309 | Sentence | denotes | This mutant has decreased levels of cell surface sialic acid relative to both wild-type CHO-K1 and Lec2 mutant CHO cells. |
T5 | 310-441 | Sentence | denotes | The CMP-sialic acid transporter (CMP-SAT) gene in the MAR-11 mutant cell has a C-T mutation that results in a premature stop codon. |
T6 | 442-605 | Sentence | denotes | As a result, MAR-11 cells express a truncated version of CMP-SAT which contains only 100 amino acids rather than the normal CMP-SAT which contains 336 amino acids. |
T7 | 606-728 | Sentence | denotes | Biochemical analyses indicate that recombinant interferon-gamma (IFN-gamma) produced by the mutant cells lack sialic acid. |
T8 | 729-832 | Sentence | denotes | Using MAR-11 as host cells, an EPO/IEF assay for the structure-function study of CMP-SAT was developed. |
T9 | 833-938 | Sentence | denotes | This assay seems more sensitive than previous assays that were used to analyze sialylation in Lec2 cells. |
T10 | 939-1136 | Sentence | denotes | Cotransfection of constructs that express CMP-SAT into MAR-11 cells completely converted the recombinant EPO to a sialylation pattern that is similar to the EPO produced by the wild-type CHO cells. |
T11 | 1137-1285 | Sentence | denotes | Using this assay, we showed that CMP-SAT lacking C-terminal 18 amino acids from the cytosolic tail was able to allow high levels of EPO sialylation. |
T12 | 1286-1437 | Sentence | denotes | Substitution of the Gly residues with Ile in three different transmembrane domains of CMP-SAT resulted in dramatic decreases in transporter's activity. |
T13 | 1438-1649 | Sentence | denotes | The CMP-SAT only lost partial activity if the same Gly residues were substituted with Ala, suggesting that the lack of side chain in Gly residues in the transmembrane domains is essential for transport activity. |
GlycoBiology-FMA
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
_T1 | 224-236 | FMAID:200942 | denotes | cell surface |
_T2 | 224-236 | FMAID:212684 | denotes | cell surface |
_T3 | 229-236 | FMAID:146300 | denotes | surface |
_T4 | 229-236 | FMAID:50594 | denotes | surface |
_T5 | 303-308 | FMAID:169002 | denotes | cells |
_T6 | 303-308 | FMAID:68646 | denotes | cells |
_T7 | 352-356 | FMAID:198663 | denotes | gene |
_T8 | 462-467 | FMAID:68646 | denotes | cells |
_T9 | 462-467 | FMAID:169002 | denotes | cells |
_T10 | 531-542 | FMAID:82739 | denotes | amino acids |
_T11 | 531-542 | FMAID:196728 | denotes | amino acids |
_T12 | 593-604 | FMAID:82739 | denotes | amino acids |
_T13 | 593-604 | FMAID:196728 | denotes | amino acids |
_T14 | 705-710 | FMAID:169002 | denotes | cells |
_T15 | 705-710 | FMAID:68646 | denotes | cells |
_T16 | 750-755 | FMAID:169002 | denotes | cells |
_T17 | 750-755 | FMAID:68646 | denotes | cells |
_T18 | 932-937 | FMAID:68646 | denotes | cells |
_T19 | 932-937 | FMAID:169002 | denotes | cells |
_T20 | 1001-1006 | FMAID:169002 | denotes | cells |
_T21 | 1001-1006 | FMAID:68646 | denotes | cells |
_T22 | 1130-1135 | FMAID:169002 | denotes | cells |
_T23 | 1130-1135 | FMAID:68646 | denotes | cells |
_T24 | 1200-1211 | FMAID:82739 | denotes | amino acids |
_T25 | 1200-1211 | FMAID:196728 | denotes | amino acids |
_T26 | 1231-1235 | FMAID:228776 | denotes | tail |
uniprot-human
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 10-13 | http://www.uniprot.org/uniprot/P21941 | denotes | CMP |
T2 | 314-317 | http://www.uniprot.org/uniprot/P21941 | denotes | CMP |
T3 | 343-346 | http://www.uniprot.org/uniprot/P21941 | denotes | CMP |
T4 | 499-502 | http://www.uniprot.org/uniprot/P21941 | denotes | CMP |
T5 | 566-569 | http://www.uniprot.org/uniprot/P21941 | denotes | CMP |
T6 | 810-813 | http://www.uniprot.org/uniprot/P21941 | denotes | CMP |
T7 | 981-984 | http://www.uniprot.org/uniprot/P21941 | denotes | CMP |
T8 | 1170-1173 | http://www.uniprot.org/uniprot/P21941 | denotes | CMP |
T9 | 1372-1375 | http://www.uniprot.org/uniprot/P21941 | denotes | CMP |
T10 | 1442-1445 | http://www.uniprot.org/uniprot/P21941 | denotes | CMP |
T11 | 14-37 | http://www.uniprot.org/uniprot/P78382 | denotes | sialic acid transporter |
T12 | 318-346 | http://www.uniprot.org/uniprot/P78382 | denotes | sialic acid transporter (CMP |
T13 | 280-282 | http://www.uniprot.org/uniprot/P04264 | denotes | K1 |
T14 | 347-350 | http://www.uniprot.org/uniprot/P21673 | denotes | SAT |
T15 | 503-506 | http://www.uniprot.org/uniprot/P21673 | denotes | SAT |
T16 | 570-573 | http://www.uniprot.org/uniprot/P21673 | denotes | SAT |
T17 | 814-817 | http://www.uniprot.org/uniprot/P21673 | denotes | SAT |
T18 | 985-988 | http://www.uniprot.org/uniprot/P21673 | denotes | SAT |
T19 | 1174-1177 | http://www.uniprot.org/uniprot/P21673 | denotes | SAT |
T20 | 1376-1379 | http://www.uniprot.org/uniprot/P21673 | denotes | SAT |
T21 | 1446-1449 | http://www.uniprot.org/uniprot/P21673 | denotes | SAT |
T22 | 347-350 | http://www.uniprot.org/uniprot/O43252 | denotes | SAT |
T23 | 503-506 | http://www.uniprot.org/uniprot/O43252 | denotes | SAT |
T24 | 570-573 | http://www.uniprot.org/uniprot/O43252 | denotes | SAT |
T25 | 814-817 | http://www.uniprot.org/uniprot/O43252 | denotes | SAT |
T26 | 985-988 | http://www.uniprot.org/uniprot/O43252 | denotes | SAT |
T27 | 1174-1177 | http://www.uniprot.org/uniprot/O43252 | denotes | SAT |
T28 | 1376-1379 | http://www.uniprot.org/uniprot/O43252 | denotes | SAT |
T29 | 1446-1449 | http://www.uniprot.org/uniprot/O43252 | denotes | SAT |
T30 | 347-350 | http://www.uniprot.org/uniprot/O95340 | denotes | SAT |
T31 | 503-506 | http://www.uniprot.org/uniprot/O95340 | denotes | SAT |
T32 | 570-573 | http://www.uniprot.org/uniprot/O95340 | denotes | SAT |
T33 | 814-817 | http://www.uniprot.org/uniprot/O95340 | denotes | SAT |
T34 | 985-988 | http://www.uniprot.org/uniprot/O95340 | denotes | SAT |
T35 | 1174-1177 | http://www.uniprot.org/uniprot/O95340 | denotes | SAT |
T36 | 1376-1379 | http://www.uniprot.org/uniprot/O95340 | denotes | SAT |
T37 | 1446-1449 | http://www.uniprot.org/uniprot/O95340 | denotes | SAT |
T38 | 664-674 | http://www.uniprot.org/uniprot/P01579 | denotes | gamma (IFN |
T39 | 760-763 | http://www.uniprot.org/uniprot/P11678 | denotes | EPO |
T40 | 1044-1047 | http://www.uniprot.org/uniprot/P11678 | denotes | EPO |
T41 | 1096-1099 | http://www.uniprot.org/uniprot/P11678 | denotes | EPO |
T42 | 1269-1272 | http://www.uniprot.org/uniprot/P11678 | denotes | EPO |
T43 | 760-763 | http://www.uniprot.org/uniprot/P01588 | denotes | EPO |
T44 | 1044-1047 | http://www.uniprot.org/uniprot/P01588 | denotes | EPO |
T45 | 1096-1099 | http://www.uniprot.org/uniprot/P01588 | denotes | EPO |
T46 | 1269-1272 | http://www.uniprot.org/uniprot/P01588 | denotes | EPO |
uniprot-mouse
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 280-282 | http://www.uniprot.org/uniprot/P04104 | denotes | K1 |
T2 | 287-291 | http://www.uniprot.org/uniprot/Q80TR1 | denotes | Lec2 |
T3 | 927-931 | http://www.uniprot.org/uniprot/Q80TR1 | denotes | Lec2 |
T4 | 318-346 | http://www.uniprot.org/uniprot/Q61420 | denotes | sialic acid transporter (CMP |
T5 | 347-350 | http://www.uniprot.org/uniprot/O88428 | denotes | SAT |
T6 | 503-506 | http://www.uniprot.org/uniprot/O88428 | denotes | SAT |
T7 | 570-573 | http://www.uniprot.org/uniprot/O88428 | denotes | SAT |
T8 | 814-817 | http://www.uniprot.org/uniprot/O88428 | denotes | SAT |
T9 | 985-988 | http://www.uniprot.org/uniprot/O88428 | denotes | SAT |
T10 | 1174-1177 | http://www.uniprot.org/uniprot/O88428 | denotes | SAT |
T11 | 1376-1379 | http://www.uniprot.org/uniprot/O88428 | denotes | SAT |
T12 | 1446-1449 | http://www.uniprot.org/uniprot/O88428 | denotes | SAT |
T13 | 347-350 | http://www.uniprot.org/uniprot/Q60967 | denotes | SAT |
T14 | 503-506 | http://www.uniprot.org/uniprot/Q60967 | denotes | SAT |
T15 | 570-573 | http://www.uniprot.org/uniprot/Q60967 | denotes | SAT |
T16 | 814-817 | http://www.uniprot.org/uniprot/Q60967 | denotes | SAT |
T17 | 985-988 | http://www.uniprot.org/uniprot/Q60967 | denotes | SAT |
T18 | 1174-1177 | http://www.uniprot.org/uniprot/Q60967 | denotes | SAT |
T19 | 1376-1379 | http://www.uniprot.org/uniprot/Q60967 | denotes | SAT |
T20 | 1446-1449 | http://www.uniprot.org/uniprot/Q60967 | denotes | SAT |
T21 | 664-674 | http://www.uniprot.org/uniprot/P01580 | denotes | gamma (IFN |
T22 | 760-763 | http://www.uniprot.org/uniprot/P49290 | denotes | EPO |
T23 | 1044-1047 | http://www.uniprot.org/uniprot/P49290 | denotes | EPO |
T24 | 1096-1099 | http://www.uniprot.org/uniprot/P49290 | denotes | EPO |
T25 | 1269-1272 | http://www.uniprot.org/uniprot/P49290 | denotes | EPO |
GlycoBiology-NCBITAXON
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 152-159 | http://purl.bioontology.org/ontology/NCBITAXON/37500 | denotes | Maackia |
T2 | 152-169 | http://purl.bioontology.org/ontology/NCBITAXON/37501 | denotes | Maackia amurensis |
T3 | 303-308 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
T4 | 435-440 | http://purl.bioontology.org/ontology/NCBITAXON/79338 | denotes | codon |
T5 | 435-440 | http://purl.bioontology.org/ontology/NCBITAXON/722432 | denotes | codon |
T6 | 462-467 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
T7 | 705-710 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
T8 | 750-755 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
T9 | 932-937 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
T10 | 1001-1006 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
T11 | 1130-1135 | http://purl.bioontology.org/ontology/STY/T025 | denotes | cells |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 14-37 | http://purl.obolibrary.org/obo/GO_0015739 | denotes | sialic acid transporter |
T2 | 318-341 | http://purl.obolibrary.org/obo/GO_0015739 | denotes | sialic acid transporter |
T3 | 14-37 | http://purl.obolibrary.org/obo/GO_0015782 | denotes | sialic acid transporter |
T4 | 318-346 | http://purl.obolibrary.org/obo/GO_0015782 | denotes | sialic acid transporter (CMP |
T5 | 26-37 | http://purl.obolibrary.org/obo/GO_0006810 | denotes | transporter |
T6 | 330-341 | http://purl.obolibrary.org/obo/GO_0006810 | denotes | transporter |
T7 | 1414-1427 | http://purl.obolibrary.org/obo/GO_0006810 | denotes | transporter's |
T8 | 1630-1639 | http://purl.obolibrary.org/obo/GO_0006810 | denotes | transport |
T9 | 45-48 | http://purl.obolibrary.org/obo/GO_0043848 | denotes | CHO |
T10 | 88-91 | http://purl.obolibrary.org/obo/GO_0043848 | denotes | CHO |
T11 | 276-279 | http://purl.obolibrary.org/obo/GO_0043848 | denotes | CHO |
T12 | 299-302 | http://purl.obolibrary.org/obo/GO_0043848 | denotes | CHO |
T13 | 1126-1129 | http://purl.obolibrary.org/obo/GO_0043848 | denotes | CHO |
T14 | 822-831 | http://purl.obolibrary.org/obo/GO_0032502 | denotes | developed |
T15 | 855-864 | http://purl.obolibrary.org/obo/GO_0046960 | denotes | sensitive |
T16 | 912-923 | http://purl.obolibrary.org/obo/GO_0097503 | denotes | sialylation |
T17 | 1053-1064 | http://purl.obolibrary.org/obo/GO_0097503 | denotes | sialylation |
T18 | 1273-1284 | http://purl.obolibrary.org/obo/GO_0097503 | denotes | sialylation |
T19 | 1414-1436 | http://purl.obolibrary.org/obo/GO_0005215 | denotes | transporter's activity |
T20 | 1630-1648 | http://purl.obolibrary.org/obo/GO_0005215 | denotes | transport activity |
T21 | 1414-1436 | http://purl.obolibrary.org/obo/GO_0015291 | denotes | transporter's activity |
T22 | 1630-1648 | http://purl.obolibrary.org/obo/GO_0015291 | denotes | transport activity |
T23 | 1414-1436 | http://purl.obolibrary.org/obo/GO_0051050 | denotes | transporter's activity |
T24 | 1630-1648 | http://purl.obolibrary.org/obo/GO_0051050 | denotes | transport activity |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 653-669 | http://purl.obolibrary.org/obo/GO_0005133 | denotes | interferon-gamma |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 4-9 | http://purl.obolibrary.org/obo/GO_0005794 | denotes | Golgi |
T2 | 224-228 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
T3 | 303-308 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T4 | 462-467 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T5 | 705-710 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T6 | 750-755 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T7 | 932-937 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T8 | 1001-1006 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T9 | 1130-1135 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T10 | 224-236 | http://purl.obolibrary.org/obo/GO_0009986 | denotes | cell surface |
T11 | 745-749 | http://purl.obolibrary.org/obo/GO_0018995 | denotes | host |
T12 | 745-755 | http://purl.obolibrary.org/obo/GO_0043657 | denotes | host cells |
T13 | 1221-1230 | http://purl.obolibrary.org/obo/GO_0005829 | denotes | cytosolic |
T14 | 1347-1360 | http://purl.obolibrary.org/obo/GO_0016021 | denotes | transmembrane |
T15 | 1347-1360 | http://purl.obolibrary.org/obo/GO_0044214 | denotes | transmembrane |
T16 | 1591-1604 | http://purl.obolibrary.org/obo/GO_0016021 | denotes | transmembrane |
T17 | 1591-1604 | http://purl.obolibrary.org/obo/GO_0044214 | denotes | transmembrane |
UBERON-AE
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 1231-1235 | http://purl.obolibrary.org/obo/UBERON_0002415 | denotes | tail |
performance-test
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
PD-UBERON-AE-B_T1 | 1231-1235 | http://purl.obolibrary.org/obo/UBERON_0002415 | denotes | tail |
mondo_disease
Id | Subject | Object | Predicate | Lexical cue | mondo_id |
---|---|---|---|---|---|
T1 | 105-108 | Disease | denotes | MAR | http://purl.obolibrary.org/obo/MONDO_0007925 |
T2 | 364-367 | Disease | denotes | MAR | http://purl.obolibrary.org/obo/MONDO_0007925 |
T3 | 455-458 | Disease | denotes | MAR | http://purl.obolibrary.org/obo/MONDO_0007925 |
T4 | 735-738 | Disease | denotes | MAR | http://purl.obolibrary.org/obo/MONDO_0007925 |
T5 | 994-997 | Disease | denotes | MAR | http://purl.obolibrary.org/obo/MONDO_0007925 |
Lectin-Jamboree-Sentence
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T1 | 0-85 | Sentence | denotes | The Golgi CMP-sialic acid transporter: A new CHO mutant provides functional insights. |
T2 | 86-187 | Sentence | denotes | A CHO mutant line, MAR-11, was isolated using a cytotoxic lectin, Maackia amurensis agglutinin (MAA). |
T3 | 188-309 | Sentence | denotes | This mutant has decreased levels of cell surface sialic acid relative to both wild-type CHO-K1 and Lec2 mutant CHO cells. |
T4 | 310-441 | Sentence | denotes | The CMP-sialic acid transporter (CMP-SAT) gene in the MAR-11 mutant cell has a C-T mutation that results in a premature stop codon. |
T5 | 442-605 | Sentence | denotes | As a result, MAR-11 cells express a truncated version of CMP-SAT which contains only 100 amino acids rather than the normal CMP-SAT which contains 336 amino acids. |
T6 | 606-728 | Sentence | denotes | Biochemical analyses indicate that recombinant interferon-gamma (IFN-gamma) produced by the mutant cells lack sialic acid. |
T7 | 729-832 | Sentence | denotes | Using MAR-11 as host cells, an EPO/IEF assay for the structure-function study of CMP-SAT was developed. |
T8 | 833-938 | Sentence | denotes | This assay seems more sensitive than previous assays that were used to analyze sialylation in Lec2 cells. |
T9 | 939-1136 | Sentence | denotes | Cotransfection of constructs that express CMP-SAT into MAR-11 cells completely converted the recombinant EPO to a sialylation pattern that is similar to the EPO produced by the wild-type CHO cells. |
T10 | 1137-1285 | Sentence | denotes | Using this assay, we showed that CMP-SAT lacking C-terminal 18 amino acids from the cytosolic tail was able to allow high levels of EPO sialylation. |
T11 | 1286-1437 | Sentence | denotes | Substitution of the Gly residues with Ile in three different transmembrane domains of CMP-SAT resulted in dramatic decreases in transporter's activity. |
T12 | 1438-1649 | Sentence | denotes | The CMP-SAT only lost partial activity if the same Gly residues were substituted with Ala, suggesting that the lack of side chain in Gly residues in the transmembrane domains is essential for transport activity. |
NCBITAXON
Id | Subject | Object | Predicate | Lexical cue | db_id |
---|---|---|---|---|---|
T1 | 152-169 | OrganismTaxon | denotes | Maackia amurensis | 37501 |
Anatomy-UBERON
Id | Subject | Object | Predicate | Lexical cue | uberon_id |
---|---|---|---|---|---|
T1 | 4-9 | Body_part | denotes | Golgi | http://purl.obolibrary.org/obo/GO_0005794 |
T2 | 1231-1235 | Body_part | denotes | tail | http://purl.obolibrary.org/obo/UBERON_0002415|http://purl.obolibrary.org/obo/UBERON_4000164 |
T4 | 1347-1360 | Body_part | denotes | transmembrane | http://purl.obolibrary.org/obo/GO_0016020 |
T5 | 1591-1604 | Body_part | denotes | transmembrane | http://purl.obolibrary.org/obo/GO_0016020 |