PubMed:18495929 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/18495929","sourcedb":"PubMed","sourceid":"18495929","source_url":"https://www.ncbi.nlm.nih.gov/pubmed/18495929","text":"Deletion of a gene encoding an amino acid transporter in the midgut membrane causes resistance to a Bombyx parvo-like virus.\nBombyx mori densovirus type 2 (BmDNV-2), a parvo-like virus, replicates only in midgut columnar cells and causes fatal disease. The resistance expressed in some silkworm strains against the virus is determined by a single gene, nsd-2, which is characterized as nonsusceptibility irrespective of the viral dose. However, the responsible gene has been unknown. We isolated the nsd-2 gene by positional cloning. The virus resistance is caused by a 6-kb deletion in the ORF of a gene encoding a 12-pass transmembrane protein, a member of an amino acid transporter family, and expressed only in midgut. Germ-line transformation with a wild-type transgene expressed in the midgut restores susceptibility, showing that the defective membrane protein is responsible for resistance. Cumulatively, our data show that the membrane protein is a functional receptor for BmDNV-2. This is a previously undescribed report of positional cloning of a mutant gene in Bombyx and isolation of an absolute virus resistance gene in insects.","tracks":[{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":107,"end":123},"obj":"Species:45454"},{"id":"T2","span":{"begin":125,"end":147},"obj":"Species:10809"},{"id":"T3","span":{"begin":156,"end":163},"obj":"Species:45454"},{"id":"T4","span":{"begin":168,"end":184},"obj":"Species:45454"},{"id":"T5","span":{"begin":238,"end":251},"obj":"Disease:MESH:D034062"},{"id":"T6","span":{"begin":286,"end":294},"obj":"Species:7091"},{"id":"T7","span":{"begin":353,"end":358},"obj":"Gene:100174816"},{"id":"T8","span":{"begin":500,"end":505},"obj":"Gene:100174816"},{"id":"T9","span":{"begin":982,"end":989},"obj":"Species:45454"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"},{"subj":"T9","pred":"source","obj":"silkworm"}]},{"project":"silkwormbase","denotations":[{"id":null,"span":{"begin":353,"end":358},"obj":"BMO_00481"},{"id":null,"span":{"begin":500,"end":505},"obj":"BMO_00481"},{"id":"T9","span":{"begin":982,"end":989},"obj":"Species:45454"},{"id":"T8","span":{"begin":500,"end":505},"obj":"Gene:100174816"},{"id":"T7","span":{"begin":353,"end":358},"obj":"Gene:100174816"},{"id":"T6","span":{"begin":286,"end":294},"obj":"Species:7091"},{"id":"T5","span":{"begin":238,"end":251},"obj":"Disease:MESH:D034062"},{"id":"T4","span":{"begin":168,"end":184},"obj":"Species:45454"},{"id":"T3","span":{"begin":156,"end":163},"obj":"Species:45454"},{"id":"T2","span":{"begin":125,"end":147},"obj":"Species:10809"},{"id":"T1","span":{"begin":107,"end":123},"obj":"Species:45454"}],"attributes":[{"subj":null,"pred":"source","obj":"silkwormbase"},{"subj":null,"pred":"source","obj":"silkwormbase"},{"subj":"T9","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T1","pred":"source","obj":"silkwormbase"}]},{"project":"silkworm_phenotype","denotations":[{"id":null,"span":{"begin":500,"end":505},"obj":"BMO_00481"},{"id":null,"span":{"begin":353,"end":358},"obj":"BMO_00481"},{"id":"T1","span":{"begin":107,"end":123},"obj":"Species:45454"},{"id":"T2","span":{"begin":125,"end":147},"obj":"Species:10809"},{"id":"T3","span":{"begin":156,"end":163},"obj":"Species:45454"},{"id":"T4","span":{"begin":168,"end":184},"obj":"Species:45454"},{"id":"T5","span":{"begin":238,"end":251},"obj":"Disease:MESH:D034062"},{"id":"T6","span":{"begin":286,"end":294},"obj":"Species:7091"},{"id":"T7","span":{"begin":353,"end":358},"obj":"Gene:100174816"},{"id":"T8","span":{"begin":500,"end":505},"obj":"Gene:100174816"},{"id":"T9","span":{"begin":982,"end":989},"obj":"Species:45454"}],"attributes":[{"subj":null,"pred":"source","obj":"silkworm_phenotype"},{"subj":null,"pred":"source","obj":"silkworm_phenotype"},{"subj":"T1","pred":"source","obj":"silkworm_phenotype"},{"subj":"T2","pred":"source","obj":"silkworm_phenotype"},{"subj":"T3","pred":"source","obj":"silkworm_phenotype"},{"subj":"T4","pred":"source","obj":"silkworm_phenotype"},{"subj":"T5","pred":"source","obj":"silkworm_phenotype"},{"subj":"T6","pred":"source","obj":"silkworm_phenotype"},{"subj":"T7","pred":"source","obj":"silkworm_phenotype"},{"subj":"T8","pred":"source","obj":"silkworm_phenotype"},{"subj":"T9","pred":"source","obj":"silkworm_phenotype"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"silkworm","color":"#93e6ec","default":true},{"id":"silkwormbase","color":"#ecd893"},{"id":"silkworm_phenotype","color":"#be93ec"}]}]}}