PubMed:18458331 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/18458331","sourcedb":"PubMed","sourceid":"18458331","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/18458331","text":"COI1 is a critical component of a receptor for jasmonate and the bacterial virulence factor coronatine.\nJasmonate (JA) is a lipid-derived hormone that regulates diverse aspects of plant immunity and development. An amino acid-conjugated form of JA, jasmonoyl-isoleucine (JA-Ile), stimulates binding of the F-box protein coronatine-insensitive 1 (COI1) to, and subsequent ubiquitin-dependent degradation of, jasmonate ZIM domain (JAZ) proteins that repress transcription of JA-responsive genes. The virulence factor coronatine (COR), which is produced by plant pathogenic strains of Pseudomonas syringae, suppresses host defense responses by activating JA signaling in a COI1-dependent manner. Although previous data indicate that COR acts as a molecular mimic of JA-Ile, the mechanism by which JA-Ile and COR are perceived by plant cells remains unknown. Here, we show that interaction of tomato COI1 with divergent members of the JAZ family is highly specific for JA-Ile and structurally related JA conjugates and that COR is approximately 1,000-fold more active than JA-Ile in promoting this interaction in vitro. JA-Ile competes for binding of COR to COI1-JAZ complexes, demonstrating that COR and JA-Ile are recognized by the same receptor. Binding of COR to the COI1-JAZ complex requires COI1 and is severely impaired by a point mutation in the putative ligand-binding pocket of COI1. Finally, we show that the C-terminal region of JAZ3 containing the highly conserved Jas motif is necessary and sufficient for hormone-induced COI1-JAZ interaction. These findings demonstrate that COI1 is a critical component of the JA receptor and that COR exerts its virulence effects by functioning as a potent agonist of this receptor system.","tracks":[{"project":"Trait curation","denotations":[{"id":"T1","span":{"begin":0,"end":4},"obj":"111559"},{"id":"T2","span":{"begin":0,"end":4},"obj":"112852"},{"id":"T3","span":{"begin":115,"end":117},"obj":"144994"},{"id":"T4","span":{"begin":245,"end":247},"obj":"144994"},{"id":"T5","span":{"begin":271,"end":273},"obj":"144994"},{"id":"T6","span":{"begin":346,"end":350},"obj":"112852"},{"id":"T7","span":{"begin":346,"end":350},"obj":"111559"},{"id":"T8","span":{"begin":417,"end":420},"obj":"1654619"},{"id":"T9","span":{"begin":473,"end":475},"obj":"144994"},{"id":"T10","span":{"begin":652,"end":654},"obj":"144994"},{"id":"T11","span":{"begin":670,"end":674},"obj":"111559"},{"id":"T12","span":{"begin":670,"end":674},"obj":"112852"},{"id":"T13","span":{"begin":734,"end":738},"obj":"663"},{"id":"T14","span":{"begin":763,"end":765},"obj":"144994"},{"id":"T15","span":{"begin":794,"end":796},"obj":"144994"},{"id":"T16","span":{"begin":896,"end":900},"obj":"111559"},{"id":"T17","span":{"begin":896,"end":900},"obj":"112852"},{"id":"T18","span":{"begin":965,"end":967},"obj":"144994"},{"id":"T19","span":{"begin":997,"end":999},"obj":"144994"},{"id":"T20","span":{"begin":1069,"end":1071},"obj":"144994"},{"id":"T21","span":{"begin":1116,"end":1118},"obj":"144994"},{"id":"T22","span":{"begin":1154,"end":1158},"obj":"111559"},{"id":"T23","span":{"begin":1154,"end":1158},"obj":"112852"},{"id":"T24","span":{"begin":1201,"end":1203},"obj":"144994"},{"id":"T25","span":{"begin":1267,"end":1271},"obj":"111559"},{"id":"T26","span":{"begin":1267,"end":1271},"obj":"112852"},{"id":"T27","span":{"begin":1293,"end":1297},"obj":"112852"},{"id":"T28","span":{"begin":1293,"end":1297},"obj":"111559"},{"id":"T29","span":{"begin":1384,"end":1388},"obj":"112852"},{"id":"T30","span":{"begin":1384,"end":1388},"obj":"111559"},{"id":"T31","span":{"begin":1437,"end":1441},"obj":"145034"},{"id":"T32","span":{"begin":1474,"end":1477},"obj":"144994"},{"id":"T33","span":{"begin":1532,"end":1536},"obj":"112852"},{"id":"T34","span":{"begin":1532,"end":1536},"obj":"111559"},{"id":"T35","span":{"begin":1586,"end":1590},"obj":"111559"},{"id":"T36","span":{"begin":1586,"end":1590},"obj":"112852"},{"id":"T37","span":{"begin":1622,"end":1624},"obj":"144994"}],"attributes":[{"subj":"T1","pred":"source","obj":"Trait curation"},{"subj":"T2","pred":"source","obj":"Trait curation"},{"subj":"T3","pred":"source","obj":"Trait curation"},{"subj":"T4","pred":"source","obj":"Trait curation"},{"subj":"T5","pred":"source","obj":"Trait curation"},{"subj":"T6","pred":"source","obj":"Trait curation"},{"subj":"T7","pred":"source","obj":"Trait curation"},{"subj":"T8","pred":"source","obj":"Trait curation"},{"subj":"T9","pred":"source","obj":"Trait curation"},{"subj":"T10","pred":"source","obj":"Trait curation"},{"subj":"T11","pred":"source","obj":"Trait curation"},{"subj":"T12","pred":"source","obj":"Trait curation"},{"subj":"T13","pred":"source","obj":"Trait curation"},{"subj":"T14","pred":"source","obj":"Trait curation"},{"subj":"T15","pred":"source","obj":"Trait curation"},{"subj":"T16","pred":"source","obj":"Trait curation"},{"subj":"T17","pred":"source","obj":"Trait curation"},{"subj":"T18","pred":"source","obj":"Trait curation"},{"subj":"T19","pred":"source","obj":"Trait curation"},{"subj":"T20","pred":"source","obj":"Trait curation"},{"subj":"T21","pred":"source","obj":"Trait curation"},{"subj":"T22","pred":"source","obj":"Trait curation"},{"subj":"T23","pred":"source","obj":"Trait curation"},{"subj":"T24","pred":"source","obj":"Trait curation"},{"subj":"T25","pred":"source","obj":"Trait curation"},{"subj":"T26","pred":"source","obj":"Trait curation"},{"subj":"T27","pred":"source","obj":"Trait curation"},{"subj":"T28","pred":"source","obj":"Trait curation"},{"subj":"T29","pred":"source","obj":"Trait curation"},{"subj":"T30","pred":"source","obj":"Trait curation"},{"subj":"T31","pred":"source","obj":"Trait curation"},{"subj":"T32","pred":"source","obj":"Trait curation"},{"subj":"T33","pred":"source","obj":"Trait curation"},{"subj":"T34","pred":"source","obj":"Trait curation"},{"subj":"T35","pred":"source","obj":"Trait curation"},{"subj":"T36","pred":"source","obj":"Trait curation"},{"subj":"T37","pred":"source","obj":"Trait curation"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"Trait curation","color":"#93ecbf","default":true}]}]}}