PubMed:18421178 JSONTXT

{"target":"http://pubannotation.org/docs/sourcedb/PubMed/sourceid/18421178","sourcedb":"PubMed","sourceid":"18421178","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/18421178","text":"Direct comparison between ICSI-mediated DNA transfer and pronuclear DNA microinjection for producing transgenic rats.\nProduction efficiency of transgenic rats was compared directly between the routine pronuclear microinjection of exogenous DNA solution (PNMI-Tg method) and the ooplasmic injection of sperm cells exposed to exogenous DNA solution (ICSI-Tg method) using six DNA constructs. The overall production efficiency per treated oocyte in the ICSI-Tg method (mean 1.1%, range 0.2 to 3.1%) was similar to that in the PNMI-Tg method (mean 1.1%, range 0 to 2.4%). An advantage of the ICSI-Tg method in the production of transgenic rats is noted in cases in which a low yield of pronuclear zygotes is an inevitable fate of the rat strain.","tracks":[]}