PubMed:18305173 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/18305173","sourcedb":"PubMed","sourceid":"18305173","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/18305173","text":"MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation.\nIn response to Toll-like receptor ligands, dendritic cells (DCs) dramatically enhance their antigen presentation capacity by stabilizing at the cell-surface MHC II molecules. We demonstrate here that, in human monocyte-derived DCs, the RING-CH ubiquitin E3 ligase, membrane-associated RING-CH I (MARCH I), promotes the ubiquitination of the HLA-DR beta-chain. Thus, in nonactivated DCs, MARCH I induces the surface internalization of mature HLA-DR complexes, therefore reducing their stability and levels. We further demonstrate that the maturation-dependent down-regulation of MARCH I is a key event in MHC class II up-regulation at the surface of LPS-activated DCs. MARCH I is, therefore, a major regulator of HLA-DR traffic, and its loss contributes to the acquisition of the potent immunostimulatory properties of mature human DCs.","tracks":[{"project":"LocText","denotations":[{"id":"T1","span":{"begin":34,"end":41},"obj":"go:GO:0009986"},{"id":"T2","span":{"begin":45,"end":50},"obj":"taxonomy:9606"},{"id":"T3","span":{"begin":105,"end":112},"obj":"uniprot:Q8TCQ1"},{"id":"T4","span":{"begin":274,"end":286},"obj":"go:GO:0009986"},{"id":"T5","span":{"begin":334,"end":339},"obj":"taxonomy:9606"},{"id":"T6","span":{"begin":395,"end":424},"obj":"uniprot:Q8TCQ1"},{"id":"T7","span":{"begin":426,"end":433},"obj":"uniprot:Q8TCQ1"},{"id":"T8","span":{"begin":517,"end":524},"obj":"uniprot:Q8TCQ1"},{"id":"T9","span":{"begin":537,"end":544},"obj":"go:GO:0009986"},{"id":"T10","span":{"begin":708,"end":715},"obj":"uniprot:Q8TCQ1"},{"id":"T11","span":{"begin":768,"end":775},"obj":"go:GO:0009986"},{"id":"T12","span":{"begin":798,"end":805},"obj":"uniprot:Q8TCQ1"},{"id":"T13","span":{"begin":955,"end":960},"obj":"taxonomy:9606"}],"namespaces":[{"prefix":"uniprot","uri":"http://identifiers.org/uniprot/"},{"prefix":"taxonomy","uri":"http://identifiers.org/taxonomy/"},{"prefix":"go","uri":"http://identifiers.org/go/"}],"attributes":[{"subj":"T1","pred":"source","obj":"LocText"},{"subj":"T2","pred":"source","obj":"LocText"},{"subj":"T3","pred":"source","obj":"LocText"},{"subj":"T4","pred":"source","obj":"LocText"},{"subj":"T5","pred":"source","obj":"LocText"},{"subj":"T6","pred":"source","obj":"LocText"},{"subj":"T7","pred":"source","obj":"LocText"},{"subj":"T8","pred":"source","obj":"LocText"},{"subj":"T9","pred":"source","obj":"LocText"},{"subj":"T10","pred":"source","obj":"LocText"},{"subj":"T11","pred":"source","obj":"LocText"},{"subj":"T12","pred":"source","obj":"LocText"},{"subj":"T13","pred":"source","obj":"LocText"}]},{"project":"PMID_GLOBAL","denotations":[{"id":"T1","span":{"begin":105,"end":110},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T2","span":{"begin":190,"end":193},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T3","span":{"begin":357,"end":360},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T4","span":{"begin":371,"end":373},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T5","span":{"begin":420,"end":422},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T6","span":{"begin":426,"end":431},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T7","span":{"begin":512,"end":515},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T8","span":{"begin":517,"end":522},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T9","span":{"begin":708,"end":713},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T10","span":{"begin":779,"end":782},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T11","span":{"begin":793,"end":796},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T12","span":{"begin":798,"end":803},"obj":"DiseaseOrPhenotypicFeature"},{"id":"T13","span":{"begin":961,"end":964},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"0009359"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"0010155"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"0010155"},{"id":"A4","pred":"mondo_id","subj":"T4","obj":"0008090"},{"id":"A5","pred":"mondo_id","subj":"T5","obj":"0008090"},{"id":"A6","pred":"mondo_id","subj":"T6","obj":"0009359"},{"id":"A7","pred":"mondo_id","subj":"T7","obj":"0010155"},{"id":"A8","pred":"mondo_id","subj":"T8","obj":"0009359"},{"id":"A9","pred":"mondo_id","subj":"T9","obj":"0009359"},{"id":"A10","pred":"mondo_id","subj":"T10","obj":"0019508"},{"id":"A11","pred":"mondo_id","subj":"T11","obj":"0010155"},{"id":"A12","pred":"mondo_id","subj":"T12","obj":"0009359"},{"id":"A13","pred":"mondo_id","subj":"T13","obj":"0010155"},{"subj":"T1","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T2","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T3","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T4","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T5","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T6","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T7","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T8","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T9","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T10","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T11","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T12","pred":"source","obj":"PMID_GLOBAL"},{"subj":"T13","pred":"source","obj":"PMID_GLOBAL"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LocText","color":"#ec93d9","default":true},{"id":"PMID_GLOBAL","color":"#93ece5"}]}]}}