PubMed:18247116
Annnotations
GlyCosmos6-Glycan-Motif-Image
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 24-35 | Glycan_Motif | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
| T2 | 68-79 | Glycan_Motif | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
| T3 | 139-150 | Glycan_Motif | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
| T4 | 152-154 | Glycan_Motif | denotes | CH | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
| T5 | 291-313 | Glycan_Motif | denotes | N-acetyl-galactosamine | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G27025MB |
| T6 | 397-399 | Glycan_Motif | denotes | CH | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
| T7 | 821-823 | Glycan_Motif | denotes | CH | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
| T8 | 892-894 | Glycan_Motif | denotes | CH | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
| T9 | 899-901 | Glycan_Motif | denotes | CH | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
| T10 | 1324-1326 | Glycan_Motif | denotes | CH | https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G43702JT |
Glycosmos6-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 24-35 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | chondroitin |
| T2 | 68-79 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | chondroitin |
| T3 | 139-150 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | chondroitin |
| T4 | 152-154 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | CH |
| T5 | 397-399 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | CH |
| T6 | 821-823 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | CH |
| T7 | 892-894 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | CH |
| T8 | 899-901 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | CH |
| T9 | 1324-1326 | http://www.glycoepitope.jp/epitopes/EP0081 | denotes | CH |
GlyCosmos6-Glycan-Motif-Structure
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 24-35 | https://glytoucan.org/Structures/Glycans/G43702JT | denotes | chondroitin |
| T2 | 68-79 | https://glytoucan.org/Structures/Glycans/G43702JT | denotes | chondroitin |
| T3 | 139-150 | https://glytoucan.org/Structures/Glycans/G43702JT | denotes | chondroitin |
| T4 | 291-313 | https://glytoucan.org/Structures/Glycans/G27025MB | denotes | N-acetyl-galactosamine |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| TextSentencer_T1 | 0-99 | Sentence | denotes | Sequential synthesis of chondroitin oligosaccharides by immobilized chondroitin polymerase mutants. |
| TextSentencer_T2 | 100-236 | Sentence | denotes | Escherichia coli strain K4 expresses a chondroitin (CH)-polymerizing enzyme (K4CP) that contains two glycosyltransferase active domains. |
| TextSentencer_T3 | 237-415 | Sentence | denotes | K4CP alternately transfers glucuronic acid (GlcA) and N-acetyl-galactosamine (GalNAc) residues using UDP-GlcA and UDP-GalNAc donors to the nonreducing end of a CH chain acceptor. |
| TextSentencer_T4 | 416-692 | Sentence | denotes | Here we generated two K4CP point mutants substituted at the UDP-sugar binding motif (DXD) in the glycosyltransferase active domains, which showed either glycosyltransferase activity of the intact domain and retained comparable activity after immobilization onto agarose beads. |
| TextSentencer_T5 | 693-930 | Sentence | denotes | The mutant enzyme-immobilized beads exhibited an addition of GlcA or GalNAc to GalNAc or GlcA residue at the nonreducing end of CH oligosaccharides and sequentially elongated pyridylamine-conjugated CH (PA-CH) chain by the alternate use. |
| TextSentencer_T6 | 931-1286 | Sentence | denotes | The sequential elongation up to 16-mer was successfully achieved as assessed by fluorescent detection on a gel filtration chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and MALDI potential lift tandem TOF mass spectrometry (MALDI-LIFT-TOF/TOF MS/MS) analyses in the negative reflection mode. |
| TextSentencer_T7 | 1287-1416 | Sentence | denotes | This method provides exactly defined CH oligosaccharide derivatives, which are useful for studies on glycosaminoglycan functions. |
| T1 | 0-99 | Sentence | denotes | Sequential synthesis of chondroitin oligosaccharides by immobilized chondroitin polymerase mutants. |
| T2 | 100-236 | Sentence | denotes | Escherichia coli strain K4 expresses a chondroitin (CH)-polymerizing enzyme (K4CP) that contains two glycosyltransferase active domains. |
| T3 | 237-415 | Sentence | denotes | K4CP alternately transfers glucuronic acid (GlcA) and N-acetyl-galactosamine (GalNAc) residues using UDP-GlcA and UDP-GalNAc donors to the nonreducing end of a CH chain acceptor. |
| T4 | 416-692 | Sentence | denotes | Here we generated two K4CP point mutants substituted at the UDP-sugar binding motif (DXD) in the glycosyltransferase active domains, which showed either glycosyltransferase activity of the intact domain and retained comparable activity after immobilization onto agarose beads. |
| T5 | 693-930 | Sentence | denotes | The mutant enzyme-immobilized beads exhibited an addition of GlcA or GalNAc to GalNAc or GlcA residue at the nonreducing end of CH oligosaccharides and sequentially elongated pyridylamine-conjugated CH (PA-CH) chain by the alternate use. |
| T6 | 931-1286 | Sentence | denotes | The sequential elongation up to 16-mer was successfully achieved as assessed by fluorescent detection on a gel filtration chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and MALDI potential lift tandem TOF mass spectrometry (MALDI-LIFT-TOF/TOF MS/MS) analyses in the negative reflection mode. |
| T7 | 1287-1416 | Sentence | denotes | This method provides exactly defined CH oligosaccharide derivatives, which are useful for studies on glycosaminoglycan functions. |
Glycan-GlyCosmos
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 24-35 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T2 | 68-79 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T3 | 139-150 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T4 | 152-154 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T5 | 315-321 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T6 | 355-361 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T7 | 397-399 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T8 | 762-768 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T9 | 772-778 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T10 | 821-823 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T11 | 892-894 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T12 | 899-901 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T13 | 1324-1326 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
GlyCosmos-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 24-35 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T2 | 68-79 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T3 | 139-150 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T4 | 152-154 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
| T5 | 397-399 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
| T6 | 821-823 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
| T7 | 892-894 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
| T8 | 899-901 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
| T9 | 1324-1326 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
GlyCosmos15-NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 100-116 | OrganismTaxon | denotes | Escherichia coli | 562 |
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| TextSentencer_T1 | 0-99 | Sentence | denotes | Sequential synthesis of chondroitin oligosaccharides by immobilized chondroitin polymerase mutants. |
| TextSentencer_T2 | 100-236 | Sentence | denotes | Escherichia coli strain K4 expresses a chondroitin (CH)-polymerizing enzyme (K4CP) that contains two glycosyltransferase active domains. |
| TextSentencer_T3 | 237-415 | Sentence | denotes | K4CP alternately transfers glucuronic acid (GlcA) and N-acetyl-galactosamine (GalNAc) residues using UDP-GlcA and UDP-GalNAc donors to the nonreducing end of a CH chain acceptor. |
| TextSentencer_T4 | 416-692 | Sentence | denotes | Here we generated two K4CP point mutants substituted at the UDP-sugar binding motif (DXD) in the glycosyltransferase active domains, which showed either glycosyltransferase activity of the intact domain and retained comparable activity after immobilization onto agarose beads. |
| TextSentencer_T5 | 693-930 | Sentence | denotes | The mutant enzyme-immobilized beads exhibited an addition of GlcA or GalNAc to GalNAc or GlcA residue at the nonreducing end of CH oligosaccharides and sequentially elongated pyridylamine-conjugated CH (PA-CH) chain by the alternate use. |
| TextSentencer_T6 | 931-1286 | Sentence | denotes | The sequential elongation up to 16-mer was successfully achieved as assessed by fluorescent detection on a gel filtration chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and MALDI potential lift tandem TOF mass spectrometry (MALDI-LIFT-TOF/TOF MS/MS) analyses in the negative reflection mode. |
| TextSentencer_T7 | 1287-1416 | Sentence | denotes | This method provides exactly defined CH oligosaccharide derivatives, which are useful for studies on glycosaminoglycan functions. |
| T1 | 0-99 | Sentence | denotes | Sequential synthesis of chondroitin oligosaccharides by immobilized chondroitin polymerase mutants. |
| T2 | 100-236 | Sentence | denotes | Escherichia coli strain K4 expresses a chondroitin (CH)-polymerizing enzyme (K4CP) that contains two glycosyltransferase active domains. |
| T3 | 237-415 | Sentence | denotes | K4CP alternately transfers glucuronic acid (GlcA) and N-acetyl-galactosamine (GalNAc) residues using UDP-GlcA and UDP-GalNAc donors to the nonreducing end of a CH chain acceptor. |
| T4 | 416-692 | Sentence | denotes | Here we generated two K4CP point mutants substituted at the UDP-sugar binding motif (DXD) in the glycosyltransferase active domains, which showed either glycosyltransferase activity of the intact domain and retained comparable activity after immobilization onto agarose beads. |
| T5 | 693-930 | Sentence | denotes | The mutant enzyme-immobilized beads exhibited an addition of GlcA or GalNAc to GalNAc or GlcA residue at the nonreducing end of CH oligosaccharides and sequentially elongated pyridylamine-conjugated CH (PA-CH) chain by the alternate use. |
| T6 | 931-1286 | Sentence | denotes | The sequential elongation up to 16-mer was successfully achieved as assessed by fluorescent detection on a gel filtration chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and MALDI potential lift tandem TOF mass spectrometry (MALDI-LIFT-TOF/TOF MS/MS) analyses in the negative reflection mode. |
| T7 | 1287-1416 | Sentence | denotes | This method provides exactly defined CH oligosaccharide derivatives, which are useful for studies on glycosaminoglycan functions. |
GlyCosmos15-Sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-99 | Sentence | denotes | Sequential synthesis of chondroitin oligosaccharides by immobilized chondroitin polymerase mutants. |
| T2 | 100-236 | Sentence | denotes | Escherichia coli strain K4 expresses a chondroitin (CH)-polymerizing enzyme (K4CP) that contains two glycosyltransferase active domains. |
| T3 | 237-415 | Sentence | denotes | K4CP alternately transfers glucuronic acid (GlcA) and N-acetyl-galactosamine (GalNAc) residues using UDP-GlcA and UDP-GalNAc donors to the nonreducing end of a CH chain acceptor. |
| T4 | 416-692 | Sentence | denotes | Here we generated two K4CP point mutants substituted at the UDP-sugar binding motif (DXD) in the glycosyltransferase active domains, which showed either glycosyltransferase activity of the intact domain and retained comparable activity after immobilization onto agarose beads. |
| T5 | 693-930 | Sentence | denotes | The mutant enzyme-immobilized beads exhibited an addition of GlcA or GalNAc to GalNAc or GlcA residue at the nonreducing end of CH oligosaccharides and sequentially elongated pyridylamine-conjugated CH (PA-CH) chain by the alternate use. |
| T6 | 931-1286 | Sentence | denotes | The sequential elongation up to 16-mer was successfully achieved as assessed by fluorescent detection on a gel filtration chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and MALDI potential lift tandem TOF mass spectrometry (MALDI-LIFT-TOF/TOF MS/MS) analyses in the negative reflection mode. |
| T7 | 1287-1416 | Sentence | denotes | This method provides exactly defined CH oligosaccharide derivatives, which are useful for studies on glycosaminoglycan functions. |
GlyCosmos15-Glycan
| Id | Subject | Object | Predicate | Lexical cue | image |
|---|---|---|---|---|---|
| T1 | 24-35 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T2 | 68-79 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T3 | 139-150 | Glycan | denotes | chondroitin | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T4 | 152-154 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T5 | 315-321 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T6 | 355-361 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T7 | 397-399 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T8 | 762-768 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T9 | 772-778 | Glycan | denotes | GalNAc | https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL |
| T10 | 821-823 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T11 | 892-894 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T12 | 899-901 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
| T13 | 1324-1326 | Glycan | denotes | CH | https://api.glycosmos.org/wurcs2image/latest/png/binary/G43702JT |
GlyCosmos15-GlycoEpitope
| Id | Subject | Object | Predicate | Lexical cue | glycoepitope_id |
|---|---|---|---|---|---|
| T1 | 24-35 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T2 | 68-79 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T3 | 139-150 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | chondroitin | http://www.glycoepitope.jp/epitopes/EP0081 |
| T4 | 152-154 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
| T5 | 397-399 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
| T6 | 821-823 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
| T7 | 892-894 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
| T8 | 899-901 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
| T9 | 1324-1326 | http://purl.jp/bio/12/glyco/glycan#Glycan_epitope | denotes | CH | http://www.glycoepitope.jp/epitopes/EP0081 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 100-116 | OrganismTaxon | denotes | Escherichia coli | 562 |