| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-127 |
Sentence |
denotes |
CCN3/nephroblastoma overexpressed matricellular protein regulates integrin expression, adhesion, and dissemination in melanoma. |
| TextSentencer_T2 |
128-325 |
Sentence |
denotes |
CCN3/nephroblastoma overexpressed belongs to the CCN family of genes that encode secreted proteins associated with the extracellular matrix (ECM) and exert regulatory effects at the cellular level. |
| TextSentencer_T3 |
326-451 |
Sentence |
denotes |
Overexpression of CCN3 was shown in metastatic melanoma cells compared with cells of the primary tumor from the same patient. |
| TextSentencer_T4 |
452-667 |
Sentence |
denotes |
Analysis of short-term cultures from 50 primary and metastatic melanomas revealed a heterogeneous expression pattern of both the 46-kDa full-length cytoplasmic/secreted protein and the 32-kDa nuclear-truncated form. |
| TextSentencer_T5 |
668-769 |
Sentence |
denotes |
The different protein expression patterns were not associated with gene alterations or polymorphisms. |
| TextSentencer_T6 |
770-1029 |
Sentence |
denotes |
Like the metastatic cells expressing high levels of the 46-kDa CCN3, cells transfected to overexpress CCN3 showed increased adhesion to ECM proteins, whereas inhibition of CCN3 expression by small interfering RNA decreased adhesion to laminin and vitronectin. |
| TextSentencer_T7 |
1030-1195 |
Sentence |
denotes |
CCN3 overexpression induced increased expression of laminin and vitronectin integrin receptors alpha 7 beta 1 and alpha v beta 5 by increasing their mRNA production. |
| TextSentencer_T8 |
1196-1304 |
Sentence |
denotes |
Moreover, CCN3 secreted by melanoma cells acted as an adhesion matrix protein for melanoma cells themselves. |
| TextSentencer_T9 |
1305-1573 |
Sentence |
denotes |
Analysis of CCN3 protein expression with respect to melanoma progression detected the protein in all visceral metastases tested and in most nodal metastases from relapsing patients but in only a few nodal metastases from nonrelapsing patients and cutaneous metastases. |
| TextSentencer_T10 |
1574-1707 |
Sentence |
denotes |
Consistently, xenotransplantation in immunodeficient mice showed a higher metastatic potential of melanoma cells overexpressing CCN3. |
| TextSentencer_T11 |
1708-1921 |
Sentence |
denotes |
Together, these data indicate a role for CCN3 in melanoma cell interaction with the ECM by regulating integrin expression, resulting in altered cell adhesion and leading melanoma progression to aggressive disease. |
| T1 |
0-127 |
Sentence |
denotes |
CCN3/nephroblastoma overexpressed matricellular protein regulates integrin expression, adhesion, and dissemination in melanoma. |
| T2 |
128-325 |
Sentence |
denotes |
CCN3/nephroblastoma overexpressed belongs to the CCN family of genes that encode secreted proteins associated with the extracellular matrix (ECM) and exert regulatory effects at the cellular level. |
| T3 |
326-451 |
Sentence |
denotes |
Overexpression of CCN3 was shown in metastatic melanoma cells compared with cells of the primary tumor from the same patient. |
| T4 |
452-667 |
Sentence |
denotes |
Analysis of short-term cultures from 50 primary and metastatic melanomas revealed a heterogeneous expression pattern of both the 46-kDa full-length cytoplasmic/secreted protein and the 32-kDa nuclear-truncated form. |
| T5 |
668-769 |
Sentence |
denotes |
The different protein expression patterns were not associated with gene alterations or polymorphisms. |
| T6 |
770-1029 |
Sentence |
denotes |
Like the metastatic cells expressing high levels of the 46-kDa CCN3, cells transfected to overexpress CCN3 showed increased adhesion to ECM proteins, whereas inhibition of CCN3 expression by small interfering RNA decreased adhesion to laminin and vitronectin. |
| T7 |
1030-1195 |
Sentence |
denotes |
CCN3 overexpression induced increased expression of laminin and vitronectin integrin receptors alpha 7 beta 1 and alpha v beta 5 by increasing their mRNA production. |
| T8 |
1196-1304 |
Sentence |
denotes |
Moreover, CCN3 secreted by melanoma cells acted as an adhesion matrix protein for melanoma cells themselves. |
| T9 |
1305-1573 |
Sentence |
denotes |
Analysis of CCN3 protein expression with respect to melanoma progression detected the protein in all visceral metastases tested and in most nodal metastases from relapsing patients but in only a few nodal metastases from nonrelapsing patients and cutaneous metastases. |
| T10 |
1574-1707 |
Sentence |
denotes |
Consistently, xenotransplantation in immunodeficient mice showed a higher metastatic potential of melanoma cells overexpressing CCN3. |
| T11 |
1708-1921 |
Sentence |
denotes |
Together, these data indicate a role for CCN3 in melanoma cell interaction with the ECM by regulating integrin expression, resulting in altered cell adhesion and leading melanoma progression to aggressive disease. |