PubMed:1823160 JSONTXT

{"project":"Glycan-Motif","denotations":[{"id":"T1","span":{"begin":19,"end":40},"obj":"https://glytoucan.org/Structures/Glycans/G27025MB"},{"id":"T2","span":{"begin":871,"end":882},"obj":"https://glytoucan.org/Structures/Glycans/G81533KY"},{"id":"T3","span":{"begin":1105,"end":1124},"obj":"https://glytoucan.org/Structures/Glycans/G00055MO"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":19,"end":40},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":871,"end":882},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":1105,"end":1124},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G27025MB"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G81533KY"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00055MO"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":838,"end":846},"obj":"http://purl.obolibrary.org/obo/MAT_0000086"},{"id":"T2","span":{"begin":976,"end":984},"obj":"http://purl.obolibrary.org/obo/MAT_0000086"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":159},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":160,"end":443},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":444,"end":581},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":582,"end":707},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":708,"end":922},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":923,"end":985},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":986,"end":1051},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1052,"end":1254},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1255,"end":1428},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":159},"obj":"Sentence"},{"id":"T2","span":{"begin":160,"end":443},"obj":"Sentence"},{"id":"T3","span":{"begin":444,"end":581},"obj":"Sentence"},{"id":"T4","span":{"begin":582,"end":707},"obj":"Sentence"},{"id":"T5","span":{"begin":708,"end":922},"obj":"Sentence"},{"id":"T6","span":{"begin":923,"end":985},"obj":"Sentence"},{"id":"T7","span":{"begin":986,"end":1051},"obj":"Sentence"},{"id":"T8","span":{"begin":1052,"end":1254},"obj":"Sentence"},{"id":"T9","span":{"begin":1255,"end":1428},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":159},"obj":"Sentence"},{"id":"T2","span":{"begin":160,"end":443},"obj":"Sentence"},{"id":"T3","span":{"begin":444,"end":581},"obj":"Sentence"},{"id":"T4","span":{"begin":582,"end":707},"obj":"Sentence"},{"id":"T5","span":{"begin":708,"end":922},"obj":"Sentence"},{"id":"T6","span":{"begin":923,"end":985},"obj":"Sentence"},{"id":"T7","span":{"begin":986,"end":1051},"obj":"Sentence"},{"id":"T8","span":{"begin":1052,"end":1254},"obj":"Sentence"},{"id":"T9","span":{"begin":1255,"end":1428},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":19,"end":40},"obj":"https://glytoucan.org/Structures/Glycans/G27025MB"},{"id":"T2","span":{"begin":871,"end":882},"obj":"https://glytoucan.org/Structures/Glycans/G81533KY"},{"id":"T3","span":{"begin":1105,"end":1124},"obj":"https://glytoucan.org/Structures/Glycans/G00055MO"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"DisGeNET","denotations":[{"id":"T0","span":{"begin":130,"end":158},"obj":"gene:100128998"},{"id":"T1","span":{"begin":121,"end":129},"obj":"disease:C0025202"},{"id":"T2","span":{"begin":218,"end":221},"obj":"gene:2187"},{"id":"T3","span":{"begin":415,"end":423},"obj":"disease:C0025202"},{"id":"T4","span":{"begin":218,"end":221},"obj":"gene:2187"},{"id":"T5","span":{"begin":275,"end":283},"obj":"disease:C0025202"}],"relations":[{"id":"R1","pred":"associated_with","subj":"T0","obj":"T1"},{"id":"R2","pred":"associated_with","subj":"T2","obj":"T3"},{"id":"R3","pred":"associated_with","subj":"T4","obj":"T5"}],"namespaces":[{"prefix":"gene","uri":"http://www.ncbi.nlm.nih.gov/gene/"},{"prefix":"disease","uri":"http://purl.bioontology.org/ontology/MEDLINEPLUS/"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":19,"end":40},"obj":"FMAID:196780"},{"id":"_T2","span":{"begin":19,"end":40},"obj":"FMAID:82786"},{"id":"_T3","span":{"begin":52,"end":67},"obj":"FMAID:196731"},{"id":"_T4","span":{"begin":52,"end":67},"obj":"FMAID:82742"},{"id":"_T5","span":{"begin":130,"end":136},"obj":"FMAID:256050"},{"id":"_T6","span":{"begin":249,"end":265},"obj":"FMAID:82742"},{"id":"_T7","span":{"begin":249,"end":265},"obj":"FMAID:196731"},{"id":"_T8","span":{"begin":284,"end":290},"obj":"FMAID:256050"},{"id":"_T9","span":{"begin":372,"end":377},"obj":"FMAID:7209"},{"id":"_T10","span":{"begin":372,"end":377},"obj":"FMAID:93687"},{"id":"_T11","span":{"begin":378,"end":383},"obj":"FMAID:169002"},{"id":"_T12","span":{"begin":378,"end":383},"obj":"FMAID:68646"},{"id":"_T13","span":{"begin":385,"end":387},"obj":"FMAID:272963"},{"id":"_T14","span":{"begin":401,"end":405},"obj":"FMAID:198663"},{"id":"_T15","span":{"begin":502,"end":504},"obj":"FMAID:272963"},{"id":"_T16","span":{"begin":589,"end":595},"obj":"FMAID:171168"},{"id":"_T17","span":{"begin":589,"end":595},"obj":"FMAID:30332"},{"id":"_T18","span":{"begin":596,"end":608},"obj":"FMAID:167256"},{"id":"_T19","span":{"begin":596,"end":608},"obj":"FMAID:62925"},{"id":"_T20","span":{"begin":659,"end":674},"obj":"FMAID:196731"},{"id":"_T21","span":{"begin":659,"end":674},"obj":"FMAID:82742"},{"id":"_T22","span":{"begin":730,"end":745},"obj":"FMAID:196731"},{"id":"_T23","span":{"begin":730,"end":745},"obj":"FMAID:82742"},{"id":"_T24","span":{"begin":1037,"end":1050},"obj":"FMAID:167256"},{"id":"_T25","span":{"begin":1037,"end":1050},"obj":"FMAID:62925"},{"id":"_T26","span":{"begin":1105,"end":1124},"obj":"FMAID:82786"},{"id":"_T27","span":{"begin":1105,"end":1124},"obj":"FMAID:196780"},{"id":"_T28","span":{"begin":1187,"end":1202},"obj":"FMAID:82742"},{"id":"_T29","span":{"begin":1187,"end":1202},"obj":"FMAID:196731"},{"id":"_T30","span":{"begin":1414,"end":1427},"obj":"FMAID:167256"},{"id":"_T31","span":{"begin":1414,"end":1427},"obj":"FMAID:62925"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":137,"end":148},"obj":"http://www.uniprot.org/uniprot/P00747"},{"id":"T2","span":{"begin":291,"end":302},"obj":"http://www.uniprot.org/uniprot/P00747"},{"id":"T3","span":{"begin":222,"end":224},"obj":"http://www.uniprot.org/uniprot/Q99707"},{"id":"T4","span":{"begin":1357,"end":1359},"obj":"http://www.uniprot.org/uniprot/Q99707"},{"id":"T5","span":{"begin":317,"end":319},"obj":"http://www.uniprot.org/uniprot/P04746"},{"id":"T6","span":{"begin":388,"end":390},"obj":"http://www.uniprot.org/uniprot/P04746"},{"id":"T7","span":{"begin":439,"end":441},"obj":"http://www.uniprot.org/uniprot/P04746"},{"id":"T8","span":{"begin":505,"end":507},"obj":"http://www.uniprot.org/uniprot/P04746"},{"id":"T9","span":{"begin":578,"end":580},"obj":"http://www.uniprot.org/uniprot/P04746"},{"id":"T10","span":{"begin":1094,"end":1096},"obj":"http://www.uniprot.org/uniprot/P04746"},{"id":"T11","span":{"begin":317,"end":319},"obj":"http://www.uniprot.org/uniprot/Q9UL45"},{"id":"T12","span":{"begin":388,"end":390},"obj":"http://www.uniprot.org/uniprot/Q9UL45"},{"id":"T13","span":{"begin":439,"end":441},"obj":"http://www.uniprot.org/uniprot/Q9UL45"},{"id":"T14","span":{"begin":505,"end":507},"obj":"http://www.uniprot.org/uniprot/Q9UL45"},{"id":"T15","span":{"begin":578,"end":580},"obj":"http://www.uniprot.org/uniprot/Q9UL45"},{"id":"T16","span":{"begin":1094,"end":1096},"obj":"http://www.uniprot.org/uniprot/Q9UL45"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":137,"end":148},"obj":"http://www.uniprot.org/uniprot/P20918"},{"id":"T2","span":{"begin":291,"end":302},"obj":"http://www.uniprot.org/uniprot/P20918"},{"id":"T3","span":{"begin":222,"end":224},"obj":"http://www.uniprot.org/uniprot/A6H5Y3"},{"id":"T4","span":{"begin":1357,"end":1359},"obj":"http://www.uniprot.org/uniprot/A6H5Y3"},{"id":"T5","span":{"begin":314,"end":316},"obj":"http://www.uniprot.org/uniprot/Q8R3F5"},{"id":"T6","span":{"begin":575,"end":577},"obj":"http://www.uniprot.org/uniprot/Q8R3F5"},{"id":"T7","span":{"begin":317,"end":319},"obj":"http://www.uniprot.org/uniprot/P00688"},{"id":"T8","span":{"begin":388,"end":390},"obj":"http://www.uniprot.org/uniprot/P00688"},{"id":"T9","span":{"begin":439,"end":441},"obj":"http://www.uniprot.org/uniprot/P00688"},{"id":"T10","span":{"begin":505,"end":507},"obj":"http://www.uniprot.org/uniprot/P00688"},{"id":"T11","span":{"begin":578,"end":580},"obj":"http://www.uniprot.org/uniprot/P00688"},{"id":"T12","span":{"begin":1094,"end":1096},"obj":"http://www.uniprot.org/uniprot/P00688"},{"id":"T13","span":{"begin":687,"end":690},"obj":"http://www.uniprot.org/uniprot/Q61024"},{"id":"T14","span":{"begin":699,"end":702},"obj":"http://www.uniprot.org/uniprot/Q61024"},{"id":"T15","span":{"begin":1146,"end":1149},"obj":"http://www.uniprot.org/uniprot/Q61024"},{"id":"T16","span":{"begin":1158,"end":1161},"obj":"http://www.uniprot.org/uniprot/Q61024"},{"id":"T17","span":{"begin":1241,"end":1244},"obj":"http://www.uniprot.org/uniprot/Q61024"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":121,"end":129},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/1369386"},{"id":"T2","span":{"begin":121,"end":129},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/45308"},{"id":"T3","span":{"begin":130,"end":136},"obj":"http://purl.bioontology.org/ontology/STY/T024"},{"id":"T4","span":{"begin":275,"end":283},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/45308"},{"id":"T5","span":{"begin":275,"end":283},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/1369386"},{"id":"T6","span":{"begin":284,"end":290},"obj":"http://purl.bioontology.org/ontology/STY/T024"},{"id":"T7","span":{"begin":378,"end":383},"obj":"http://purl.bioontology.org/ontology/STY/T025"},{"id":"T8","span":{"begin":415,"end":423},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/45308"},{"id":"T9","span":{"begin":415,"end":423},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/1369386"},{"id":"T10","span":{"begin":838,"end":846},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/293542"},{"id":"T11","span":{"begin":976,"end":984},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/293542"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":8,"end":18},"obj":"http://purl.obolibrary.org/obo/GO_0097503"},{"id":"T2","span":{"begin":946,"end":956},"obj":"http://purl.obolibrary.org/obo/GO_0097503"},{"id":"T3","span":{"begin":990,"end":1000},"obj":"http://purl.obolibrary.org/obo/GO_0097503"},{"id":"T4","span":{"begin":137,"end":158},"obj":"http://purl.obolibrary.org/obo/GO_0031639"},{"id":"T5","span":{"begin":291,"end":312},"obj":"http://purl.obolibrary.org/obo/GO_0031639"},{"id":"T6","span":{"begin":177,"end":181},"obj":"http://purl.obolibrary.org/obo/GO_0033867"},{"id":"T7","span":{"begin":385,"end":387},"obj":"http://purl.obolibrary.org/obo/GO_0003964"},{"id":"T8","span":{"begin":502,"end":504},"obj":"http://purl.obolibrary.org/obo/GO_0003964"},{"id":"T9","span":{"begin":762,"end":773},"obj":"http://purl.obolibrary.org/obo/GO_0036065"},{"id":"T10","span":{"begin":1028,"end":1050},"obj":"http://purl.obolibrary.org/obo/GO_0006491"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GO-CC","denotations":[{"id":"T1","span":{"begin":340,"end":343},"obj":"http://purl.obolibrary.org/obo/GO_0000407"},{"id":"T2","span":{"begin":378,"end":383},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T3","span":{"begin":424,"end":428},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4","span":{"begin":786,"end":790},"obj":"http://purl.obolibrary.org/obo/GO_0019013"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"UBERON-AE","denotations":[{"id":"T1","span":{"begin":130,"end":136},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T2","span":{"begin":284,"end":290},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"T3","span":{"begin":838,"end":846},"obj":"http://purl.obolibrary.org/obo/UBERON_0000972"},{"id":"T4","span":{"begin":976,"end":984},"obj":"http://purl.obolibrary.org/obo/UBERON_0000972"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"EDAM-topics","denotations":[{"id":"T1","span":{"begin":199,"end":216},"obj":"http://edamontology.org/topic_0134"},{"id":"T2","span":{"begin":199,"end":216},"obj":"http://edamontology.org/topic_3520"},{"id":"T3","span":{"begin":450,"end":457},"obj":"http://edamontology.org/topic_3678"},{"id":"T4","span":{"begin":1260,"end":1265},"obj":"http://edamontology.org/topic_3678"},{"id":"T5","span":{"begin":1292,"end":1310},"obj":"http://edamontology.org/topic_3520"},{"id":"T6","span":{"begin":1292,"end":1310},"obj":"http://edamontology.org/topic_0134"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":94,"end":103},"obj":"http://edamontology.org/data_0883"},{"id":"T2","span":{"begin":199,"end":216},"obj":"http://edamontology.org/data_2536"},{"id":"T3","span":{"begin":199,"end":216},"obj":"http://edamontology.org/data_3147"},{"id":"T4","span":{"begin":487,"end":497},"obj":"http://edamontology.org/data_0883"},{"id":"T5","span":{"begin":487,"end":501},"obj":"http://edamontology.org/operation_1848"},{"id":"T6","span":{"begin":551,"end":561},"obj":"http://edamontology.org/data_0883"},{"id":"T7","span":{"begin":551,"end":570},"obj":"http://edamontology.org/data_2085"},{"id":"T8","span":{"begin":551,"end":570},"obj":"http://edamontology.org/data_0887"},{"id":"T9","span":{"begin":562,"end":570},"obj":"http://edamontology.org/data_2048"},{"id":"T10","span":{"begin":617,"end":627},"obj":"http://edamontology.org/data_2611"},{"id":"T11","span":{"begin":617,"end":627},"obj":"http://edamontology.org/data_0842"},{"id":"T12","span":{"begin":636,"end":645},"obj":"http://edamontology.org/data_0883"},{"id":"T13","span":{"begin":1076,"end":1086},"obj":"http://edamontology.org/data_0883"},{"id":"T14","span":{"begin":1076,"end":1089},"obj":"http://edamontology.org/data_1070"},{"id":"T15","span":{"begin":1266,"end":1278},"obj":"http://edamontology.org/operation_2246"},{"id":"T16","span":{"begin":1292,"end":1310},"obj":"http://edamontology.org/data_2536"},{"id":"T17","span":{"begin":1364,"end":1373},"obj":"http://edamontology.org/data_0883"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"NGLY1-deficiency","denotations":[{"id":"PD-NGLY1-deficiency-B_T1","span":{"begin":831,"end":837},"obj":"chem:24139"}],"namespaces":[{"prefix":"hgnc","uri":"https://www.genenames.org/data/gene-symbol-report/#!/hgnc_id/HGNC:"},{"prefix":"omim","uri":"https://www.omim.org/entry/"},{"prefix":"chem","uri":"https://pubchem.ncbi.nlm.nih.gov/compound/"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"DisGeNET5_gene_disease","denotations":[{"id":"1823160-0#130#158#gene100128998","span":{"begin":130,"end":158},"obj":"gene100128998"},{"id":"1823160-0#121#129#diseaseC0025202","span":{"begin":121,"end":129},"obj":"diseaseC0025202"},{"id":"1823160-1#58#61#gene2187","span":{"begin":218,"end":221},"obj":"gene2187"},{"id":"1823160-1#115#123#diseaseC0025202","span":{"begin":275,"end":283},"obj":"diseaseC0025202"},{"id":"1823160-1#255#263#diseaseC0025202","span":{"begin":415,"end":423},"obj":"diseaseC0025202"}],"relations":[{"id":"130#158#gene100128998121#129#diseaseC0025202","pred":"associated_with","subj":"1823160-0#130#158#gene100128998","obj":"1823160-0#121#129#diseaseC0025202"},{"id":"58#61#gene2187115#123#diseaseC0025202","pred":"associated_with","subj":"1823160-1#58#61#gene2187","obj":"1823160-1#115#123#diseaseC0025202"},{"id":"58#61#gene2187255#263#diseaseC0025202","pred":"associated_with","subj":"1823160-1#58#61#gene2187","obj":"1823160-1#255#263#diseaseC0025202"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlycoBiology-MAT","denotations":[{"id":"T1","span":{"begin":589,"end":595},"obj":"http://purl.obolibrary.org/obo/MAT_0000488"},{"id":"T2","span":{"begin":838,"end":846},"obj":"http://purl.obolibrary.org/obo/MAT_0000086"},{"id":"T3","span":{"begin":976,"end":984},"obj":"http://purl.obolibrary.org/obo/MAT_0000086"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlyTouCan-IUPAC","denotations":[{"id":"GlycanIUPAC_T1","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G23425WZ\""},{"id":"GlycanIUPAC_T2","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G23425WZ\""},{"id":"GlycanIUPAC_T3","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G23425WZ\""},{"id":"GlycanIUPAC_T4","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G02874VH\""},{"id":"GlycanIUPAC_T5","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G02874VH\""},{"id":"GlycanIUPAC_T6","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G02874VH\""},{"id":"GlycanIUPAC_T7","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G99699DW\""},{"id":"GlycanIUPAC_T8","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G99699DW\""},{"id":"GlycanIUPAC_T9","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G99699DW\""},{"id":"GlycanIUPAC_T10","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G22074RM\""},{"id":"GlycanIUPAC_T11","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G22074RM\""},{"id":"GlycanIUPAC_T12","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G22074RM\""},{"id":"GlycanIUPAC_T13","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G48535VZ\""},{"id":"GlycanIUPAC_T14","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G48535VZ\""},{"id":"GlycanIUPAC_T15","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G48535VZ\""},{"id":"GlycanIUPAC_T16","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G39738WL\""},{"id":"GlycanIUPAC_T17","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G39738WL\""},{"id":"GlycanIUPAC_T18","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G39738WL\""},{"id":"GlycanIUPAC_T19","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G42313WU\""},{"id":"GlycanIUPAC_T20","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G42313WU\""},{"id":"GlycanIUPAC_T21","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G42313WU\""},{"id":"GlycanIUPAC_T22","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G00393CK\""},{"id":"GlycanIUPAC_T23","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G00393CK\""},{"id":"GlycanIUPAC_T24","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G00393CK\""},{"id":"GlycanIUPAC_T25","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G42649EX\""},{"id":"GlycanIUPAC_T26","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G42649EX\""},{"id":"GlycanIUPAC_T27","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G42649EX\""},{"id":"GlycanIUPAC_T28","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G46880SB\""},{"id":"GlycanIUPAC_T29","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G46880SB\""},{"id":"GlycanIUPAC_T30","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G46880SB\""},{"id":"GlycanIUPAC_T31","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G75599IR\""},{"id":"GlycanIUPAC_T32","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G75599IR\""},{"id":"GlycanIUPAC_T33","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G75599IR\""},{"id":"GlycanIUPAC_T34","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G58985MU\""},{"id":"GlycanIUPAC_T35","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G58985MU\""},{"id":"GlycanIUPAC_T36","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G58985MU\""},{"id":"GlycanIUPAC_T37","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G92517PO\""},{"id":"GlycanIUPAC_T38","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G92517PO\""},{"id":"GlycanIUPAC_T39","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G92517PO\""},{"id":"GlycanIUPAC_T40","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G88512YL\""},{"id":"GlycanIUPAC_T41","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G88512YL\""},{"id":"GlycanIUPAC_T42","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G88512YL\""},{"id":"GlycanIUPAC_T43","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G41473NX\""},{"id":"GlycanIUPAC_T44","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G41473NX\""},{"id":"GlycanIUPAC_T45","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G41473NX\""},{"id":"GlycanIUPAC_T46","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G71089RB\""},{"id":"GlycanIUPAC_T47","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G71089RB\""},{"id":"GlycanIUPAC_T48","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G71089RB\""},{"id":"GlycanIUPAC_T49","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G73485GZ\""},{"id":"GlycanIUPAC_T50","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G73485GZ\""},{"id":"GlycanIUPAC_T51","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G73485GZ\""},{"id":"GlycanIUPAC_T52","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G61406KC\""},{"id":"GlycanIUPAC_T53","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G61406KC\""},{"id":"GlycanIUPAC_T54","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G61406KC\""},{"id":"GlycanIUPAC_T55","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G34412GZ\""},{"id":"GlycanIUPAC_T56","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G34412GZ\""},{"id":"GlycanIUPAC_T57","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G34412GZ\""},{"id":"GlycanIUPAC_T58","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G67209FP\""},{"id":"GlycanIUPAC_T59","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G67209FP\""},{"id":"GlycanIUPAC_T60","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G67209FP\""},{"id":"GlycanIUPAC_T61","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G61442IL\""},{"id":"GlycanIUPAC_T62","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G61442IL\""},{"id":"GlycanIUPAC_T63","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G61442IL\""},{"id":"GlycanIUPAC_T64","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G93729MV\""},{"id":"GlycanIUPAC_T65","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G93729MV\""},{"id":"GlycanIUPAC_T66","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G93729MV\""},{"id":"GlycanIUPAC_T67","span":{"begin":817,"end":823},"obj":"\"http://rdf.glycoinfo.org/glycan/G92144AE\""},{"id":"GlycanIUPAC_T68","span":{"begin":915,"end":921},"obj":"\"http://rdf.glycoinfo.org/glycan/G92144AE\""},{"id":"GlycanIUPAC_T69","span":{"begin":1001,"end":1007},"obj":"\"http://rdf.glycoinfo.org/glycan/G92144AE\""},{"id":"GlycanIUPAC_T70","span":{"begin":817,"end":825},"obj":"\"http://rdf.glycoinfo.org/glycan/G56558YT\""},{"id":"GlycanIUPAC_T71","span":{"begin":817,"end":825},"obj":"\"http://rdf.glycoinfo.org/glycan/G83430HE\""},{"id":"GlycanIUPAC_T72","span":{"begin":817,"end":825},"obj":"\"http://rdf.glycoinfo.org/glycan/G27025MB\""},{"id":"GlycanIUPAC_T73","span":{"begin":817,"end":825},"obj":"\"http://rdf.glycoinfo.org/glycan/G48828SZ\""},{"id":"GlycanIUPAC_T74","span":{"begin":817,"end":825},"obj":"\"http://rdf.glycoinfo.org/glycan/G70697FM\""},{"id":"GlycanIUPAC_T75","span":{"begin":817,"end":825},"obj":"\"http://rdf.glycoinfo.org/glycan/G42945ZI\""},{"id":"GlycanIUPAC_T76","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G26693XF\""},{"id":"GlycanIUPAC_T77","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G01864SU\""},{"id":"GlycanIUPAC_T78","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G17605FD\""},{"id":"GlycanIUPAC_T79","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G41950LU\""},{"id":"GlycanIUPAC_T80","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G57195RJ\""},{"id":"GlycanIUPAC_T81","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G85391SA\""},{"id":"GlycanIUPAC_T82","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G89565QL\""},{"id":"GlycanIUPAC_T83","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G80869MR\""},{"id":"GlycanIUPAC_T84","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G55978NL\""},{"id":"GlycanIUPAC_T85","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G54644LT\""},{"id":"GlycanIUPAC_T86","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G25694UG\""},{"id":"GlycanIUPAC_T87","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G25126RB\""},{"id":"GlycanIUPAC_T88","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G51848AD\""},{"id":"GlycanIUPAC_T89","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G94667GM\""},{"id":"GlycanIUPAC_T90","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G30124BO\""},{"id":"GlycanIUPAC_T91","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G82777EZ\""},{"id":"GlycanIUPAC_T92","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G10151YZ\""},{"id":"GlycanIUPAC_T93","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G17585ZM\""},{"id":"GlycanIUPAC_T94","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G04411CJ\""},{"id":"GlycanIUPAC_T95","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G38254HJ\""},{"id":"GlycanIUPAC_T96","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G75188FS\""},{"id":"GlycanIUPAC_T97","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G70374VG\""},{"id":"GlycanIUPAC_T98","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G45176LJ\""},{"id":"GlycanIUPAC_T99","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G30874VW\""},{"id":"GlycanIUPAC_T100","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G69333MI\""},{"id":"GlycanIUPAC_T101","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G10676XO\""},{"id":"GlycanIUPAC_T102","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G14843DJ\""},{"id":"GlycanIUPAC_T103","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G47546FR\""},{"id":"GlycanIUPAC_T104","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G73695ZM\""},{"id":"GlycanIUPAC_T105","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G31923TJ\""},{"id":"GlycanIUPAC_T106","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G60519EP\""},{"id":"GlycanIUPAC_T107","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G07933IA\""},{"id":"GlycanIUPAC_T108","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G40745NH\""},{"id":"GlycanIUPAC_T109","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G54496YV\""},{"id":"GlycanIUPAC_T110","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G62953SQ\""},{"id":"GlycanIUPAC_T111","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G70070AY\""},{"id":"GlycanIUPAC_T112","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G78792WC\""},{"id":"GlycanIUPAC_T113","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G25238AV\""},{"id":"GlycanIUPAC_T114","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G40510DP\""},{"id":"GlycanIUPAC_T115","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G61120TK\""},{"id":"GlycanIUPAC_T116","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G41342KV\""},{"id":"GlycanIUPAC_T117","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G90703NA\""},{"id":"GlycanIUPAC_T118","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G01591HR\""},{"id":"GlycanIUPAC_T119","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G56520XN\""},{"id":"GlycanIUPAC_T120","span":{"begin":831,"end":837},"obj":"\"http://rdf.glycoinfo.org/glycan/G81830JX\""}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":130,"end":136},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"PD-UBERON-AE-B_T2","span":{"begin":284,"end":290},"obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"PD-UBERON-AE-B_T3","span":{"begin":838,"end":846},"obj":"http://purl.obolibrary.org/obo/UBERON_0000972"},{"id":"PD-UBERON-AE-B_T4","span":{"begin":976,"end":984},"obj":"http://purl.obolibrary.org/obo/UBERON_0000972"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":121,"end":129},"obj":"Disease"},{"id":"T2","span":{"begin":275,"end":283},"obj":"Disease"},{"id":"T3","span":{"begin":415,"end":423},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005105"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005105"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0005105"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"HP-phenotype","denotations":[{"id":"T1","span":{"begin":121,"end":129},"obj":"Phenotype"},{"id":"T2","span":{"begin":275,"end":283},"obj":"Phenotype"},{"id":"T3","span":{"begin":415,"end":423},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002861"},{"id":"A2","pred":"hp_id","subj":"T2","obj":"HP:0002861"},{"id":"A3","pred":"hp_id","subj":"T3","obj":"HP:0002861"}],"namespaces":[{"prefix":"HP","uri":"http://purl.obolibrary.org/obo/HP_"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"Glycan-GlyCosmos","denotations":[{"id":"T1","span":{"begin":817,"end":823},"obj":"Glycan"},{"id":"T2","span":{"begin":915,"end":921},"obj":"Glycan"},{"id":"T3","span":{"begin":1001,"end":1007},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A4","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A5","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A6","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlyCosmos15-HP","denotations":[{"id":"T1","span":{"begin":121,"end":129},"obj":"Phenotype"},{"id":"T2","span":{"begin":275,"end":283},"obj":"Phenotype"},{"id":"T3","span":{"begin":415,"end":423},"obj":"Phenotype"}],"attributes":[{"id":"A1","pred":"hp_id","subj":"T1","obj":"HP:0002861"},{"id":"A2","pred":"hp_id","subj":"T2","obj":"HP:0002861"},{"id":"A3","pred":"hp_id","subj":"T3","obj":"HP:0002861"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlyCosmos15-MONDO","denotations":[{"id":"T1","span":{"begin":121,"end":129},"obj":"Disease"},{"id":"T2","span":{"begin":275,"end":283},"obj":"Disease"},{"id":"T3","span":{"begin":415,"end":423},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0005105"},{"id":"A2","pred":"mondo_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MONDO_0005105"},{"id":"A3","pred":"mondo_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/MONDO_0005105"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlyCosmos15-NCBITAXON","denotations":[{"id":"T1","span":{"begin":356,"end":371},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10029"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlyCosmos15-UBERON","denotations":[{"id":"T1","span":{"begin":130,"end":136},"obj":"Body_part"},{"id":"T2","span":{"begin":284,"end":290},"obj":"Body_part"},{"id":"T3","span":{"begin":372,"end":377},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000992"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlyCosmos15-MAT","denotations":[{"id":"T1","span":{"begin":838,"end":846},"obj":"Body_part"},{"id":"T2","span":{"begin":976,"end":984},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000086"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000086"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":159},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":160,"end":443},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":444,"end":581},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":582,"end":707},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":708,"end":922},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":923,"end":985},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":986,"end":1051},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1052,"end":1254},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1255,"end":1428},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":159},"obj":"Sentence"},{"id":"T2","span":{"begin":160,"end":443},"obj":"Sentence"},{"id":"T3","span":{"begin":444,"end":581},"obj":"Sentence"},{"id":"T4","span":{"begin":582,"end":707},"obj":"Sentence"},{"id":"T5","span":{"begin":708,"end":922},"obj":"Sentence"},{"id":"T6","span":{"begin":923,"end":985},"obj":"Sentence"},{"id":"T7","span":{"begin":986,"end":1051},"obj":"Sentence"},{"id":"T8","span":{"begin":1052,"end":1254},"obj":"Sentence"},{"id":"T9","span":{"begin":1255,"end":1428},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":159},"obj":"Sentence"},{"id":"T2","span":{"begin":160,"end":443},"obj":"Sentence"},{"id":"T3","span":{"begin":444,"end":581},"obj":"Sentence"},{"id":"T4","span":{"begin":582,"end":707},"obj":"Sentence"},{"id":"T5","span":{"begin":708,"end":922},"obj":"Sentence"},{"id":"T6","span":{"begin":923,"end":985},"obj":"Sentence"},{"id":"T7","span":{"begin":986,"end":1051},"obj":"Sentence"},{"id":"T8","span":{"begin":1052,"end":1254},"obj":"Sentence"},{"id":"T9","span":{"begin":1255,"end":1428},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlyCosmos15-Sentences","blocks":[{"id":"T1","span":{"begin":0,"end":159},"obj":"Sentence"},{"id":"T2","span":{"begin":160,"end":443},"obj":"Sentence"},{"id":"T3","span":{"begin":444,"end":581},"obj":"Sentence"},{"id":"T4","span":{"begin":582,"end":707},"obj":"Sentence"},{"id":"T5","span":{"begin":708,"end":922},"obj":"Sentence"},{"id":"T6","span":{"begin":923,"end":985},"obj":"Sentence"},{"id":"T7","span":{"begin":986,"end":1051},"obj":"Sentence"},{"id":"T8","span":{"begin":1052,"end":1254},"obj":"Sentence"},{"id":"T9","span":{"begin":1255,"end":1428},"obj":"Sentence"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"GlyCosmos15-Glycan","denotations":[{"id":"T1","span":{"begin":817,"end":823},"obj":"Glycan"},{"id":"T2","span":{"begin":915,"end":921},"obj":"Glycan"},{"id":"T3","span":{"begin":1001,"end":1007},"obj":"Glycan"}],"attributes":[{"id":"A1","pred":"glycosmos_id","subj":"T1","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A4","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL"},{"id":"A2","pred":"glycosmos_id","subj":"T2","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A5","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL"},{"id":"A3","pred":"glycosmos_id","subj":"T3","obj":"https://glycosmos.org/glycans/show/G39738WL"},{"id":"A6","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/latest/png/binary/G39738WL"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":356,"end":371},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10029"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":130,"end":136},"obj":"Body_part"},{"id":"T2","span":{"begin":284,"end":290},"obj":"Body_part"},{"id":"T3","span":{"begin":372,"end":377},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0000479"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000992"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}

{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":838,"end":846},"obj":"Body_part"},{"id":"T2","span":{"begin":976,"end":984},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000086"},{"id":"A2","pred":"mat_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/MAT_0000086"}],"text":"A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.\nWe have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6-position of the GalNAc. Minor constituents are sialylated on both or neither antennae. The sialylated GalNAc moiety is unique in N-linked glycoproteins. The majority of complex structures in rmt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins."}