PubMed:1823159 JSONTXT

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":137},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":138,"end":405},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":406,"end":562},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":563,"end":682},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":683,"end":882},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":883,"end":1069},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1070,"end":1166},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":137},"obj":"Sentence"},{"id":"T2","span":{"begin":138,"end":405},"obj":"Sentence"},{"id":"T3","span":{"begin":406,"end":562},"obj":"Sentence"},{"id":"T4","span":{"begin":563,"end":682},"obj":"Sentence"},{"id":"T5","span":{"begin":683,"end":882},"obj":"Sentence"},{"id":"T6","span":{"begin":883,"end":1069},"obj":"Sentence"},{"id":"T7","span":{"begin":1070,"end":1166},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":137},"obj":"Sentence"},{"id":"T2","span":{"begin":138,"end":405},"obj":"Sentence"},{"id":"T3","span":{"begin":406,"end":562},"obj":"Sentence"},{"id":"T4","span":{"begin":563,"end":682},"obj":"Sentence"},{"id":"T5","span":{"begin":683,"end":882},"obj":"Sentence"},{"id":"T6","span":{"begin":883,"end":1069},"obj":"Sentence"},{"id":"T7","span":{"begin":1070,"end":1166},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":29,"end":42},"obj":"FMAID:62925"},{"id":"_T2","span":{"begin":29,"end":42},"obj":"FMAID:167256"},{"id":"_T3","span":{"begin":348,"end":351},"obj":"FMAID:85816"},{"id":"_T4","span":{"begin":416,"end":432},"obj":"FMAID:196731"},{"id":"_T5","span":{"begin":416,"end":432},"obj":"FMAID:82742"},{"id":"_T6","span":{"begin":482,"end":495},"obj":"FMAID:62925"},{"id":"_T7","span":{"begin":482,"end":495},"obj":"FMAID:167256"},{"id":"_T8","span":{"begin":945,"end":957},"obj":"FMAID:82737"},{"id":"_T9","span":{"begin":945,"end":957},"obj":"FMAID:197276"},{"id":"_T10","span":{"begin":1003,"end":1013},"obj":"FMAID:167180"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":177,"end":180},"obj":"http://www.uniprot.org/uniprot/Q9UGI8"},{"id":"T2","span":{"begin":478,"end":481},"obj":"http://www.uniprot.org/uniprot/Q9UGI8"},{"id":"T3","span":{"begin":702,"end":705},"obj":"http://www.uniprot.org/uniprot/Q9UGI8"},{"id":"T4","span":{"begin":1056,"end":1059},"obj":"http://www.uniprot.org/uniprot/Q9UGI8"},{"id":"T5","span":{"begin":343,"end":345},"obj":"http://www.uniprot.org/uniprot/Q99707"},{"id":"T6","span":{"begin":762,"end":764},"obj":"http://www.uniprot.org/uniprot/Q99707"},{"id":"T7","span":{"begin":575,"end":587},"obj":"http://www.uniprot.org/uniprot/Q05639"},{"id":"T8","span":{"begin":640,"end":652},"obj":"http://www.uniprot.org/uniprot/Q05639"},{"id":"T9","span":{"begin":601,"end":607},"obj":"http://www.uniprot.org/uniprot/Q92988"},{"id":"T10","span":{"begin":659,"end":665},"obj":"http://www.uniprot.org/uniprot/Q92988"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":343,"end":345},"obj":"http://www.uniprot.org/uniprot/A6H5Y3"},{"id":"T2","span":{"begin":762,"end":764},"obj":"http://www.uniprot.org/uniprot/A6H5Y3"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":20,"end":28},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/333870"},{"id":"T2","span":{"begin":67,"end":75},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/6264"},{"id":"T3","span":{"begin":138,"end":146},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/6264"},{"id":"T4","span":{"begin":224,"end":229},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/9611"},{"id":"T5","span":{"begin":371,"end":379},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/68518"},{"id":"T6","span":{"begin":472,"end":477},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/9611"},{"id":"T7","span":{"begin":601,"end":605},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"},{"id":"T8","span":{"begin":601,"end":605},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T9","span":{"begin":659,"end":663},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T10","span":{"begin":659,"end":663},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"},{"id":"T11","span":{"begin":876,"end":881},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/9611"},{"id":"T12","span":{"begin":992,"end":1002},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/608684"},{"id":"T13","span":{"begin":1147,"end":1156},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/600794"},{"id":"T14","span":{"begin":1157,"end":1165},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/333870"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":111,"end":121},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T2","span":{"begin":778,"end":788},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T3","span":{"begin":844,"end":854},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T4","span":{"begin":889,"end":899},"obj":"http://purl.obolibrary.org/obo/GO_0032259"},{"id":"T5","span":{"begin":298,"end":302},"obj":"http://purl.obolibrary.org/obo/GO_0033867"},{"id":"T6","span":{"begin":348,"end":351},"obj":"http://purl.obolibrary.org/obo/GO_0034005"},{"id":"T7","span":{"begin":1147,"end":1156},"obj":"http://purl.obolibrary.org/obo/GO_0072519"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"GO-MF","denotations":[{"id":"T1","span":{"begin":1003,"end":1013},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"UBERON-AE","denotations":[{"id":"T1","span":{"begin":241,"end":247},"obj":"http://purl.obolibrary.org/obo/UBERON_0002548"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"EDAM-topics","denotations":[{"id":"T1","span":{"begin":96,"end":104},"obj":"http://edamontology.org/topic_2830"},{"id":"T2","span":{"begin":167,"end":175},"obj":"http://edamontology.org/topic_2830"},{"id":"T3","span":{"begin":320,"end":337},"obj":"http://edamontology.org/topic_3520"},{"id":"T4","span":{"begin":320,"end":337},"obj":"http://edamontology.org/topic_0134"},{"id":"T5","span":{"begin":387,"end":404},"obj":"http://edamontology.org/topic_3520"},{"id":"T6","span":{"begin":387,"end":404},"obj":"http://edamontology.org/topic_0134"},{"id":"T7","span":{"begin":813,"end":821},"obj":"http://edamontology.org/topic_2269"},{"id":"T8","span":{"begin":945,"end":957},"obj":"http://edamontology.org/topic_0152"},{"id":"T9","span":{"begin":1060,"end":1068},"obj":"http://edamontology.org/topic_2830"},{"id":"T10","span":{"begin":1075,"end":1080},"obj":"http://edamontology.org/topic_3678"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":320,"end":337},"obj":"http://edamontology.org/data_2536"},{"id":"T2","span":{"begin":320,"end":337},"obj":"http://edamontology.org/data_3147"},{"id":"T3","span":{"begin":387,"end":404},"obj":"http://edamontology.org/data_2536"},{"id":"T4","span":{"begin":387,"end":404},"obj":"http://edamontology.org/data_3147"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"GlycoBiology-MAT","denotations":[{"id":"T1","span":{"begin":76,"end":95},"obj":"http://purl.obolibrary.org/obo/MAT_0000119"},{"id":"T2","span":{"begin":76,"end":95},"obj":"http://purl.obolibrary.org/obo/MAT_0000027"},{"id":"T3","span":{"begin":76,"end":95},"obj":"http://purl.obolibrary.org/obo/MAT_0000120"},{"id":"T4","span":{"begin":76,"end":95},"obj":"http://purl.obolibrary.org/obo/MAT_0000121"},{"id":"T5","span":{"begin":147,"end":166},"obj":"http://purl.obolibrary.org/obo/MAT_0000121"},{"id":"T6","span":{"begin":147,"end":166},"obj":"http://purl.obolibrary.org/obo/MAT_0000120"},{"id":"T7","span":{"begin":147,"end":166},"obj":"http://purl.obolibrary.org/obo/MAT_0000119"},{"id":"T8","span":{"begin":147,"end":166},"obj":"http://purl.obolibrary.org/obo/MAT_0000027"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"performance-test","denotations":[{"id":"PD-UBERON-AE-B_T1","span":{"begin":241,"end":247},"obj":"http://purl.obolibrary.org/obo/UBERON_0002548"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":20,"end":28},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":67,"end":75},"obj":"OrganismTaxon"},{"id":"T3","span":{"begin":138,"end":146},"obj":"OrganismTaxon"},{"id":"T4","span":{"begin":1157,"end":1165},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"6231"},{"id":"A2","pred":"db_id","subj":"T2","obj":"6264"},{"id":"A3","pred":"db_id","subj":"T3","obj":"6264"},{"id":"A4","pred":"db_id","subj":"T4","obj":"6231"}],"text":"Characterization of nematode glycoproteins: the major O-glycans of Toxocara excretory-secretory antigens are O-methylated trisaccharides.\nToxocara excretory-secretory antigens (TES) were isolated from the culture media of T.canis and T.cati larvae and their O-glycan content was investigated using fast atom bombardment-mass spectrometry (FAB-MS), gas chromatography and electron impact mass spectrometry. The major oligosaccharides released by reductive elimination of T.canis TES glycoproteins were shown to be two, approximately equi-abundant, trisaccharides: 2-O-Me-Fucp(alpha 1----2)-4-O-Me-Galp(beta 1----3)GalNAcitol and 2-O-Me-Fucp(alpha 1----2)-Galp(beta 1----3)GalNAcitol. In contrast T.cati TES O-glycans are predominantly one component, shown by FAB-MS to be a di-O-methylated trisaccharide, which is probably identical to the di-O-methylated trisaccharide from T.canis. The O-methylated trisaccharides are strong candidates for the carbohydrate epitopes recognized by a panel of monoclonal antibodies which exhibit multiple reactivity against TES antigens. This study constitutes the first rigorous characterization of glycans from a parasitic nematode."}