PubMed:1822240 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1822240","sourcedb":"PubMed","sourceid":"1822240","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1822240","text":"Glycoprotein biosynthesis in yeast: purification and characterization of the endoplasmic reticulum Man9 processing alpha-mannosidase.\nSaccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion. The activity was solubilized by the zwitterionic detergent, 3-[(3-cholamidopropyl)dimethyl ammonio]-1-propanesulphonate (CHAPS), whose presence was necessary for maximal activity. Purification included Q-Sepharose ion-exchange chromatography, preparative isoelectric focusing and HPLC gel filtration on TSK 3000 matrix. Overall purification from post-nuclear supernatants was estimated to be 110,000-fold with a 50% recovery of activity. The purified enzyme hydrolysed Man9GlcNAc1,2 from thyroglobulin or oligosaccharide-lipid, but not invertase Man9GlcNAc, Man1 alpha 2Man1 alpha OCH3 or p-nitrophenyl-alpha-D-mannopyranoside. Conversion of thyroglobulin Man9GlcNAc to Man8GlcNAc was linear with time and enzyme concentration, with an apparent Km of 0.2 mM and a specific activity of 220 IU/mg. Glc3Man9GlcNAc2 from oligosaccharide-lipid was as good a substrate as Man9GlcNAc, but the lipid-linked Man7GlcNAc2 isomer was hydrolysed at only 10% of this rate. Hydrolysis of defined isomers of IgM and bovine thyroglobulin Man6,7,8GlcNAc indicated that, for maximal alpha 1,2-mannosidase activity, only the alpha 1,2-linked terminal mannoses on the alpha 3 branch of the Man9GlcNAc precursor were dispensable. Isomers lacking the terminal alpha 1,2-linked mannose on the alpha 6 branch were hydrolysed at only approximately 10% of the maximal rate. The enzyme exhibited a pI of 5.3 and a pH optimum at 6.5. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis in the absence of reducing agents gave a single sharp band at 66 kDa, while in the presence of beta-mercaptoethanol equimolar amounts of two peptides, one of 44 kDa and one of 23 kDa, were obtained. Sizing on Sephacryl SF300, Superose 12 and TSK 3000 provided a holoenzyme mol. wt of 60-68 kDa, indicating that the isolated active form of the Man9-alpha-mannosidase was composed of one each of the sulphydryl-bonded dissimilar peptides. The enzyme bound to concanavalin A (ConA)-Sepharose and was eluted with alpha-methylmannoside, indicating the presence of high-mannose oligosaccharides. The Man9-alpha-mannosidase required low levels of Ca2+, which could be removed by EGTA. Activity was restored by Ca2+ or Zn2+, but not by Mg2+ or Mn2+.","tracks":[{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":723,"end":726},"obj":"http://www.uniprot.org/uniprot/Q8WUA8"},{"id":"T2","span":{"begin":2126,"end":2129},"obj":"http://www.uniprot.org/uniprot/Q8WUA8"},{"id":"T3","span":{"begin":908,"end":921},"obj":"http://www.uniprot.org/uniprot/Q9UNY3"},{"id":"T4","span":{"begin":1062,"end":1075},"obj":"http://www.uniprot.org/uniprot/Q9UNY3"},{"id":"T5","span":{"begin":1427,"end":1440},"obj":"http://www.uniprot.org/uniprot/Q9UNY3"},{"id":"T6","span":{"begin":1484,"end":1505},"obj":"http://www.uniprot.org/uniprot/O60476"},{"id":"T7","span":{"begin":1484,"end":1505},"obj":"http://www.uniprot.org/uniprot/Q9UKM7"},{"id":"T8","span":{"begin":1484,"end":1505},"obj":"http://www.uniprot.org/uniprot/Q9BZQ6"},{"id":"T9","span":{"begin":1484,"end":1505},"obj":"http://www.uniprot.org/uniprot/Q5SRI9"},{"id":"T10","span":{"begin":1484,"end":1505},"obj":"http://www.uniprot.org/uniprot/Q9NR34"},{"id":"T11","span":{"begin":1484,"end":1505},"obj":"http://www.uniprot.org/uniprot/P33908"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-human"},{"subj":"T2","pred":"source","obj":"uniprot-human"},{"subj":"T3","pred":"source","obj":"uniprot-human"},{"subj":"T4","pred":"source","obj":"uniprot-human"},{"subj":"T5","pred":"source","obj":"uniprot-human"},{"subj":"T6","pred":"source","obj":"uniprot-human"},{"subj":"T7","pred":"source","obj":"uniprot-human"},{"subj":"T8","pred":"source","obj":"uniprot-human"},{"subj":"T9","pred":"source","obj":"uniprot-human"},{"subj":"T10","pred":"source","obj":"uniprot-human"},{"subj":"T11","pred":"source","obj":"uniprot-human"}]},{"project":"GlycoBiology-GDGDB","denotations":[{"id":"_T1","span":{"begin":115,"end":132},"obj":"http://acgg.asia/db/diseases/gdgdb?con_ui=CON00008"},{"id":"_T2","span":{"begin":164,"end":181},"obj":"http://acgg.asia/db/diseases/gdgdb?con_ui=CON00008"},{"id":"_T3","span":{"begin":2232,"end":2249},"obj":"http://acgg.asia/db/diseases/gdgdb?con_ui=CON00008"},{"id":"_T4","span":{"begin":2483,"end":2500},"obj":"http://acgg.asia/db/diseases/gdgdb?con_ui=CON00008"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-GDGDB"},{"subj":"_T2","pred":"source","obj":"GlycoBiology-GDGDB"},{"subj":"_T3","pred":"source","obj":"GlycoBiology-GDGDB"},{"subj":"_T4","pred":"source","obj":"GlycoBiology-GDGDB"}]},{"project":"GlycoBiology-PACDB","denotations":[{"id":"_T1","span":{"begin":134,"end":158},"obj":"http://acgg.asia/db/diseases/pacdb/lec?ids=LEC297"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-PACDB"}]},{"project":"GlycoBiology-FMA","denotations":[{"id":"_T1","span":{"begin":0,"end":12},"obj":"FMAID:62925"},{"id":"_T2","span":{"begin":0,"end":12},"obj":"FMAID:167256"},{"id":"_T3","span":{"begin":77,"end":88},"obj":"FMAID:165003"},{"id":"_T4","span":{"begin":77,"end":88},"obj":"FMAID:66856"},{"id":"_T5","span":{"begin":77,"end":98},"obj":"FMAID:66898"},{"id":"_T6","span":{"begin":77,"end":98},"obj":"FMAID:165027"},{"id":"_T7","span":{"begin":77,"end":98},"obj":"FMAID:165026"},{"id":"_T8","span":{"begin":77,"end":98},"obj":"FMAID:66897"},{"id":"_T9","span":{"begin":77,"end":98},"obj":"FMAID:80351"},{"id":"_T10","span":{"begin":77,"end":98},"obj":"FMAID:188464"},{"id":"_T11","span":{"begin":77,"end":98},"obj":"FMAID:210694"},{"id":"_T12","span":{"begin":77,"end":98},"obj":"FMAID:165144"},{"id":"_T13","span":{"begin":77,"end":98},"obj":"FMAID:199093"},{"id":"_T14","span":{"begin":77,"end":98},"obj":"FMAID:67434"},{"id":"_T15","span":{"begin":77,"end":98},"obj":"FMAID:165141"},{"id":"_T16","span":{"begin":77,"end":98},"obj":"FMAID:63842"},{"id":"_T17","span":{"begin":77,"end":98},"obj":"FMAID:210679"},{"id":"_T18","span":{"begin":77,"end":98},"obj":"FMAID:67438"},{"id":"_T19","span":{"begin":77,"end":98},"obj":"FMAID:212510"},{"id":"_T20","span":{"begin":77,"end":98},"obj":"FMAID:165142"},{"id":"_T21","span":{"begin":77,"end":98},"obj":"FMAID:165250"},{"id":"_T22","span":{"begin":77,"end":98},"obj":"FMAID:67429"},{"id":"_T23","span":{"begin":77,"end":98},"obj":"FMAID:162308"},{"id":"_T24","span":{"begin":77,"end":103},"obj":"FMAID:211269"},{"id":"_T25","span":{"begin":89,"end":98},"obj":"FMAID:7646"},{"id":"_T26","span":{"begin":89,"end":98},"obj":"FMAID:94520"},{"id":"_T27","span":{"begin":227,"end":238},"obj":"FMAID:66856"},{"id":"_T28","span":{"begin":227,"end":238},"obj":"FMAID:165003"},{"id":"_T29","span":{"begin":227,"end":248},"obj":"FMAID:80351"},{"id":"_T30","span":{"begin":227,"end":248},"obj":"FMAID:210694"},{"id":"_T31","span":{"begin":227,"end":248},"obj":"FMAID:199093"},{"id":"_T32","span":{"begin":227,"end":248},"obj":"FMAID:165144"},{"id":"_T33","span":{"begin":227,"end":248},"obj":"FMAID:165250"},{"id":"_T34","span":{"begin":227,"end":248},"obj":"FMAID:67429"},{"id":"_T35","span":{"begin":227,"end":248},"obj":"FMAID:188464"},{"id":"_T36","span":{"begin":227,"end":248},"obj":"FMAID:165026"},{"id":"_T37","span":{"begin":227,"end":248},"obj":"FMAID:66897"},{"id":"_T38","span":{"begin":227,"end":248},"obj":"FMAID:212510"},{"id":"_T39","span":{"begin":227,"end":248},"obj":"FMAID:63842"},{"id":"_T40","span":{"begin":227,"end":248},"obj":"FMAID:67438"},{"id":"_T41","span":{"begin":227,"end":248},"obj":"FMAID:165142"},{"id":"_T42","span":{"begin":227,"end":248},"obj":"FMAID:162308"},{"id":"_T43","span":{"begin":227,"end":248},"obj":"FMAID:210679"},{"id":"_T44","span":{"begin":227,"end":248},"obj":"FMAID:66898"},{"id":"_T45","span":{"begin":227,"end":248},"obj":"FMAID:165027"},{"id":"_T46","span":{"begin":227,"end":248},"obj":"FMAID:211269"},{"id":"_T47","span":{"begin":227,"end":251},"obj":"FMAID:165141"},{"id":"_T48","span":{"begin":227,"end":251},"obj":"FMAID:210706"},{"id":"_T49","span":{"begin":227,"end":251},"obj":"FMAID:67434"},{"id":"_T50","span":{"begin":239,"end":248},"obj":"FMAID:94520"},{"id":"_T51","span":{"begin":239,"end":248},"obj":"FMAID:7646"},{"id":"_T52","span":{"begin":283,"end":298},"obj":"FMAID:82742"},{"id":"_T53","span":{"begin":283,"end":298},"obj":"FMAID:196731"},{"id":"_T54","span":{"begin":360,"end":370},"obj":"FMAID:165145"},{"id":"_T55","span":{"begin":371,"end":380},"obj":"FMAID:7145"},{"id":"_T56","span":{"begin":371,"end":380},"obj":"FMAID:93573"},{"id":"_T57","span":{"begin":371,"end":380},"obj":"FMAID:30322"},{"id":"_T58","span":{"begin":371,"end":380},"obj":"FMAID:167608"},{"id":"_T59","span":{"begin":908,"end":921},"obj":"FMAID:169236"},{"id":"_T60","span":{"begin":908,"end":921},"obj":"FMAID:68833"},{"id":"_T61","span":{"begin":925,"end":940},"obj":"FMAID:82742"},{"id":"_T62","span":{"begin":925,"end":940},"obj":"FMAID:196731"},{"id":"_T63","span":{"begin":941,"end":946},"obj":"FMAID:67264"},{"id":"_T64","span":{"begin":941,"end":946},"obj":"FMAID:165448"},{"id":"_T65","span":{"begin":1062,"end":1075},"obj":"FMAID:68833"},{"id":"_T66","span":{"begin":1062,"end":1075},"obj":"FMAID:169236"},{"id":"_T67","span":{"begin":1237,"end":1252},"obj":"FMAID:82742"},{"id":"_T68","span":{"begin":1237,"end":1252},"obj":"FMAID:196731"},{"id":"_T69","span":{"begin":1253,"end":1258},"obj":"FMAID:165448"},{"id":"_T70","span":{"begin":1253,"end":1258},"obj":"FMAID:67264"},{"id":"_T71","span":{"begin":1306,"end":1311},"obj":"FMAID:165448"},{"id":"_T72","span":{"begin":1306,"end":1311},"obj":"FMAID:67264"},{"id":"_T73","span":{"begin":1373,"end":1377},"obj":"FMAID:217859"},{"id":"_T74","span":{"begin":1412,"end":1415},"obj":"FMAID:167182"},{"id":"_T75","span":{"begin":1427,"end":1440},"obj":"FMAID:68833"},{"id":"_T76","span":{"begin":1427,"end":1440},"obj":"FMAID:169236"},{"id":"_T77","span":{"begin":1551,"end":1559},"obj":"FMAID:196796"},{"id":"_T78","span":{"begin":1551,"end":1559},"obj":"FMAID:82801"},{"id":"_T79","span":{"begin":1575,"end":1581},"obj":"FMAID:226027"},{"id":"_T80","span":{"begin":1575,"end":1581},"obj":"FMAID:226028"},{"id":"_T81","span":{"begin":1674,"end":1681},"obj":"FMAID:196796"},{"id":"_T82","span":{"begin":1674,"end":1681},"obj":"FMAID:82801"},{"id":"_T83","span":{"begin":1697,"end":1703},"obj":"FMAID:226028"},{"id":"_T84","span":{"begin":1697,"end":1703},"obj":"FMAID:226027"},{"id":"_T85","span":{"begin":1761,"end":1765},"obj":"FMAID:217859"},{"id":"_T86","span":{"begin":1790,"end":1792},"obj":"FMAID:214745"},{"id":"_T87","span":{"begin":2448,"end":2455},"obj":"FMAID:82801"},{"id":"_T88","span":{"begin":2448,"end":2455},"obj":"FMAID:196796"},{"id":"_T89","span":{"begin":2456,"end":2472},"obj":"FMAID:196731"},{"id":"_T90","span":{"begin":2456,"end":2472},"obj":"FMAID:82742"},{"id":"_T91","span":{"begin":2524,"end":2527},"obj":"FMAID:272430"},{"id":"_T92","span":{"begin":2524,"end":2527},"obj":"FMAID:182506"},{"id":"_T93","span":{"begin":2524,"end":2527},"obj":"FMAID:272658"},{"id":"_T94","span":{"begin":2587,"end":2590},"obj":"FMAID:272658"},{"id":"_T95","span":{"begin":2587,"end":2590},"obj":"FMAID:272430"},{"id":"_T96","span":{"begin":2587,"end":2590},"obj":"FMAID:182506"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"attributes":[{"subj":"_T1","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T2","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T3","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T4","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T5","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T6","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T7","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T8","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T9","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T10","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T11","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T12","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T13","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T14","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T15","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T16","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T17","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T18","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T19","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T20","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T21","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T22","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T23","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T24","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T25","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T26","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T27","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T28","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T29","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T30","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T31","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T32","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T33","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T34","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T35","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T36","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T37","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T38","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T39","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T40","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T41","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T42","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T43","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T44","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T45","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T46","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T47","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T48","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T49","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T50","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T51","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T52","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T53","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T54","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T55","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T56","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T57","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T58","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T59","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T60","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T61","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T62","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T63","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T64","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T65","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T66","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T67","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T68","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T69","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T70","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T71","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T72","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T73","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T74","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T75","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T76","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T77","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T78","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T79","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T80","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T81","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T82","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T83","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T84","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T85","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T86","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T87","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T88","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T89","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T90","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T91","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T92","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T93","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T94","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T95","pred":"source","obj":"GlycoBiology-FMA"},{"subj":"_T96","pred":"source","obj":"GlycoBiology-FMA"}]},{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":908,"end":921},"obj":"http://www.uniprot.org/uniprot/O08710"},{"id":"T2","span":{"begin":1062,"end":1075},"obj":"http://www.uniprot.org/uniprot/O08710"},{"id":"T3","span":{"begin":1427,"end":1440},"obj":"http://www.uniprot.org/uniprot/O08710"},{"id":"T4","span":{"begin":978,"end":982},"obj":"http://www.uniprot.org/uniprot/Q9WU40"},{"id":"T5","span":{"begin":1932,"end":1937},"obj":"http://www.uniprot.org/uniprot/Q62504"},{"id":"T6","span":{"begin":2524,"end":2527},"obj":"http://www.uniprot.org/uniprot/P00920"},{"id":"T7","span":{"begin":2587,"end":2590},"obj":"http://www.uniprot.org/uniprot/P00920"}],"attributes":[{"subj":"T1","pred":"source","obj":"uniprot-mouse"},{"subj":"T2","pred":"source","obj":"uniprot-mouse"},{"subj":"T3","pred":"source","obj":"uniprot-mouse"},{"subj":"T4","pred":"source","obj":"uniprot-mouse"},{"subj":"T5","pred":"source","obj":"uniprot-mouse"},{"subj":"T6","pred":"source","obj":"uniprot-mouse"},{"subj":"T7","pred":"source","obj":"uniprot-mouse"}]},{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":134,"end":147},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/4895"},{"id":"T2","span":{"begin":134,"end":147},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/36034"},{"id":"T3","span":{"begin":134,"end":147},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/4891"},{"id":"T4","span":{"begin":134,"end":147},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/4930"},{"id":"T5","span":{"begin":1979,"end":1983},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/158455"},{"id":"T6","span":{"begin":1979,"end":1983},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/3554"},{"id":"T7","span":{"begin":2562,"end":2570},"obj":"http://purl.bioontology.org/ontology/STY/T052"},{"id":"T8","span":{"begin":2562,"end":2570},"obj":"http://purl.bioontology.org/ontology/NCBITAXON/190658"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T2","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T3","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T4","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T5","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T6","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T7","pred":"source","obj":"GlycoBiology-NCBITAXON"},{"subj":"T8","pred":"source","obj":"GlycoBiology-NCBITAXON"}]},{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":133},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":134,"end":419},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":420,"end":599},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":600,"end":739},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":740,"end":857},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":858,"end":1047},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1048,"end":1215},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1216,"end":1378},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1379,"end":1627},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1628,"end":1766},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":1767,"end":1824},"obj":"Sentence"},{"id":"TextSentencer_T12","span":{"begin":1825,"end":2082},"obj":"Sentence"},{"id":"TextSentencer_T13","span":{"begin":2083,"end":2320},"obj":"Sentence"},{"id":"TextSentencer_T14","span":{"begin":2321,"end":2473},"obj":"Sentence"},{"id":"TextSentencer_T15","span":{"begin":2474,"end":2561},"obj":"Sentence"},{"id":"TextSentencer_T16","span":{"begin":2562,"end":2625},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":133},"obj":"Sentence"},{"id":"T2","span":{"begin":134,"end":419},"obj":"Sentence"},{"id":"T3","span":{"begin":420,"end":599},"obj":"Sentence"},{"id":"T4","span":{"begin":600,"end":739},"obj":"Sentence"},{"id":"T5","span":{"begin":740,"end":857},"obj":"Sentence"},{"id":"T6","span":{"begin":858,"end":1047},"obj":"Sentence"},{"id":"T7","span":{"begin":1048,"end":1215},"obj":"Sentence"},{"id":"T8","span":{"begin":1216,"end":1378},"obj":"Sentence"},{"id":"T9","span":{"begin":1379,"end":1627},"obj":"Sentence"},{"id":"T10","span":{"begin":1628,"end":1766},"obj":"Sentence"},{"id":"T11","span":{"begin":1767,"end":1824},"obj":"Sentence"},{"id":"T12","span":{"begin":1825,"end":2082},"obj":"Sentence"},{"id":"T13","span":{"begin":2083,"end":2320},"obj":"Sentence"},{"id":"T14","span":{"begin":2321,"end":2473},"obj":"Sentence"},{"id":"T15","span":{"begin":2474,"end":2561},"obj":"Sentence"},{"id":"T16","span":{"begin":2562,"end":2625},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":133},"obj":"Sentence"},{"id":"T2","span":{"begin":134,"end":419},"obj":"Sentence"},{"id":"T3","span":{"begin":420,"end":599},"obj":"Sentence"},{"id":"T4","span":{"begin":600,"end":739},"obj":"Sentence"},{"id":"T5","span":{"begin":740,"end":857},"obj":"Sentence"},{"id":"T6","span":{"begin":858,"end":1047},"obj":"Sentence"},{"id":"T7","span":{"begin":1048,"end":1215},"obj":"Sentence"},{"id":"T8","span":{"begin":1216,"end":1378},"obj":"Sentence"},{"id":"T9","span":{"begin":1379,"end":1627},"obj":"Sentence"},{"id":"T10","span":{"begin":1628,"end":1766},"obj":"Sentence"},{"id":"T11","span":{"begin":1767,"end":1824},"obj":"Sentence"},{"id":"T12","span":{"begin":1825,"end":2082},"obj":"Sentence"},{"id":"T13","span":{"begin":2083,"end":2320},"obj":"Sentence"},{"id":"T14","span":{"begin":2321,"end":2473},"obj":"Sentence"},{"id":"T15","span":{"begin":2474,"end":2561},"obj":"Sentence"},{"id":"T16","span":{"begin":2562,"end":2625},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T10","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T11","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T12","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T13","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T14","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T15","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T16","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"},{"subj":"T12","pred":"source","obj":"sentences"},{"subj":"T13","pred":"source","obj":"sentences"},{"subj":"T14","pred":"source","obj":"sentences"},{"subj":"T15","pred":"source","obj":"sentences"},{"subj":"T16","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"},{"subj":"T12","pred":"source","obj":"sentences"},{"subj":"T13","pred":"source","obj":"sentences"},{"subj":"T14","pred":"source","obj":"sentences"},{"subj":"T15","pred":"source","obj":"sentences"},{"subj":"T16","pred":"source","obj":"sentences"}]},{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":0,"end":25},"obj":"http://purl.obolibrary.org/obo/GO_0009101"},{"id":"T2","span":{"begin":13,"end":25},"obj":"http://purl.obolibrary.org/obo/GO_0009058"},{"id":"T3","span":{"begin":409,"end":418},"obj":"http://purl.obolibrary.org/obo/GO_0007586"},{"id":"T4","span":{"begin":638,"end":646},"obj":"http://purl.obolibrary.org/obo/GO_0015297"},{"id":"T5","span":{"begin":1484,"end":1505},"obj":"http://purl.obolibrary.org/obo/GO_0004559"},{"id":"T6","span":{"begin":1484,"end":1505},"obj":"http://purl.obolibrary.org/obo/GO_0004571"},{"id":"T7","span":{"begin":1484,"end":1514},"obj":"http://purl.obolibrary.org/obo/GO_0004559"},{"id":"T8","span":{"begin":1494,"end":1514},"obj":"http://purl.obolibrary.org/obo/GO_0015923"},{"id":"T9","span":{"begin":1840,"end":1848},"obj":"http://purl.obolibrary.org/obo/GO_0051923"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-BP"},{"subj":"T2","pred":"source","obj":"GO-BP"},{"subj":"T3","pred":"source","obj":"GO-BP"},{"subj":"T4","pred":"source","obj":"GO-BP"},{"subj":"T5","pred":"source","obj":"GO-BP"},{"subj":"T6","pred":"source","obj":"GO-BP"},{"subj":"T7","pred":"source","obj":"GO-BP"},{"subj":"T8","pred":"source","obj":"GO-BP"},{"subj":"T9","pred":"source","obj":"GO-BP"}]},{"project":"GO-CC","denotations":[{"id":"T1","span":{"begin":77,"end":98},"obj":"http://purl.obolibrary.org/obo/GO_0005783"},{"id":"T2","span":{"begin":227,"end":248},"obj":"http://purl.obolibrary.org/obo/GO_0005783"},{"id":"T3","span":{"begin":371,"end":380},"obj":"http://purl.obolibrary.org/obo/GO_0016020"}],"attributes":[{"subj":"T1","pred":"source","obj":"GO-CC"},{"subj":"T2","pred":"source","obj":"GO-CC"},{"subj":"T3","pred":"source","obj":"GO-CC"}]},{"project":"EDAM-topics","denotations":[{"id":"T1","span":{"begin":29,"end":34},"obj":"http://edamontology.org/topic_2817"},{"id":"T2","span":{"begin":29,"end":34},"obj":"http://edamontology.org/topic_0782"},{"id":"T3","span":{"begin":77,"end":98},"obj":"http://edamontology.org/topic_0616"},{"id":"T4","span":{"begin":227,"end":248},"obj":"http://edamontology.org/topic_0616"},{"id":"T5","span":{"begin":941,"end":946},"obj":"http://edamontology.org/topic_0153"},{"id":"T6","span":{"begin":1253,"end":1258},"obj":"http://edamontology.org/topic_0153"},{"id":"T7","span":{"begin":1306,"end":1311},"obj":"http://edamontology.org/topic_0153"},{"id":"T8","span":{"begin":1542,"end":1550},"obj":"http://edamontology.org/topic_0749"},{"id":"T9","span":{"begin":1648,"end":1656},"obj":"http://edamontology.org/topic_0749"},{"id":"T10","span":{"begin":2025,"end":2033},"obj":"http://edamontology.org/topic_0154"},{"id":"T11","span":{"begin":2311,"end":2319},"obj":"http://edamontology.org/topic_0154"}],"attributes":[{"subj":"T1","pred":"source","obj":"EDAM-topics"},{"subj":"T2","pred":"source","obj":"EDAM-topics"},{"subj":"T3","pred":"source","obj":"EDAM-topics"},{"subj":"T4","pred":"source","obj":"EDAM-topics"},{"subj":"T5","pred":"source","obj":"EDAM-topics"},{"subj":"T6","pred":"source","obj":"EDAM-topics"},{"subj":"T7","pred":"source","obj":"EDAM-topics"},{"subj":"T8","pred":"source","obj":"EDAM-topics"},{"subj":"T9","pred":"source","obj":"EDAM-topics"},{"subj":"T10","pred":"source","obj":"EDAM-topics"},{"subj":"T11","pred":"source","obj":"EDAM-topics"}]},{"project":"EDAM-DFO","denotations":[{"id":"T1","span":{"begin":104,"end":114},"obj":"http://edamontology.org/operation_0004"},{"id":"T2","span":{"begin":104,"end":114},"obj":"http://edamontology.org/operation_2409"},{"id":"T3","span":{"begin":199,"end":207},"obj":"http://edamontology.org/operation_3192"},{"id":"T4","span":{"begin":502,"end":510},"obj":"http://edamontology.org/operation_3642"},{"id":"T5","span":{"begin":732,"end":738},"obj":"http://edamontology.org/data_2082"},{"id":"T6","span":{"begin":1048,"end":1058},"obj":"http://edamontology.org/operation_3434"},{"id":"T7","span":{"begin":1133,"end":1146},"obj":"http://edamontology.org/data_2140"},{"id":"T8","span":{"begin":1165,"end":1167},"obj":"http://edamontology.org/data_0910"},{"id":"T9","span":{"begin":1806,"end":1808},"obj":"http://edamontology.org/format_1997"},{"id":"T10","span":{"begin":2025,"end":2033},"obj":"http://edamontology.org/data_2906"},{"id":"T11","span":{"begin":2311,"end":2319},"obj":"http://edamontology.org/data_2906"}],"attributes":[{"subj":"T1","pred":"source","obj":"EDAM-DFO"},{"subj":"T2","pred":"source","obj":"EDAM-DFO"},{"subj":"T3","pred":"source","obj":"EDAM-DFO"},{"subj":"T4","pred":"source","obj":"EDAM-DFO"},{"subj":"T5","pred":"source","obj":"EDAM-DFO"},{"subj":"T6","pred":"source","obj":"EDAM-DFO"},{"subj":"T7","pred":"source","obj":"EDAM-DFO"},{"subj":"T8","pred":"source","obj":"EDAM-DFO"},{"subj":"T9","pred":"source","obj":"EDAM-DFO"},{"subj":"T10","pred":"source","obj":"EDAM-DFO"},{"subj":"T11","pred":"source","obj":"EDAM-DFO"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":134,"end":158},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":1420,"end":1426},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"4932"},{"id":"A2","pred":"db_id","subj":"T2","obj":"9913"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"GlyTouCan-IUPAC","denotations":[{"id":"GlycanIUPAC_T1","span":{"begin":978,"end":982},"obj":"\"http://rdf.glycoinfo.org/glycan/G48232PN\""},{"id":"GlycanIUPAC_T2","span":{"begin":978,"end":982},"obj":"\"http://rdf.glycoinfo.org/glycan/G63049JM\""},{"id":"GlycanIUPAC_T3","span":{"begin":978,"end":982},"obj":"\"http://rdf.glycoinfo.org/glycan/G43927EM\""},{"id":"GlycanIUPAC_T4","span":{"begin":978,"end":982},"obj":"\"http://rdf.glycoinfo.org/glycan/G56756HZ\""},{"id":"GlycanIUPAC_T5","span":{"begin":978,"end":982},"obj":"\"http://rdf.glycoinfo.org/glycan/G33141UW\""},{"id":"GlycanIUPAC_T6","span":{"begin":978,"end":982},"obj":"\"http://rdf.glycoinfo.org/glycan/G70323CJ\""}],"attributes":[{"subj":"GlycanIUPAC_T1","pred":"source","obj":"GlyTouCan-IUPAC"},{"subj":"GlycanIUPAC_T2","pred":"source","obj":"GlyTouCan-IUPAC"},{"subj":"GlycanIUPAC_T3","pred":"source","obj":"GlyTouCan-IUPAC"},{"subj":"GlycanIUPAC_T4","pred":"source","obj":"GlyTouCan-IUPAC"},{"subj":"GlycanIUPAC_T5","pred":"source","obj":"GlyTouCan-IUPAC"},{"subj":"GlycanIUPAC_T6","pred":"source","obj":"GlyTouCan-IUPAC"}]},{"project":"GlycoBiology-Motifs","denotations":[{"id":"T1","span":{"begin":2443,"end":2455},"obj":"http://rdf.glycoinfo.org/glycan/G00028MO"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlycoBiology-Motifs"}]},{"project":"Lectin","denotations":[{"id":"Lectin_T1","span":{"begin":2357,"end":2361},"obj":"https://acgg.asia/db/lfdb/LfDB0170"}],"attributes":[{"subj":"Lectin_T1","pred":"source","obj":"Lectin"}]},{"project":"Glycan-Motif","denotations":[{"id":"T1","span":{"begin":1551,"end":1559},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T2","span":{"begin":1674,"end":1681},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T3","span":{"begin":2448,"end":2455},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycan-Motif"},{"subj":"T2","pred":"source","obj":"Glycan-Motif"},{"subj":"T3","pred":"source","obj":"Glycan-Motif"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":1551,"end":1559},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T2","span":{"begin":1674,"end":1681},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"},{"id":"T3","span":{"begin":2448,"end":2455},"obj":"https://glytoucan.org/Structures/Glycans/G70323CJ"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":1551,"end":1559},"obj":"Glycan_Motif"},{"id":"T2","span":{"begin":1674,"end":1681},"obj":"Glycan_Motif"},{"id":"T3","span":{"begin":2448,"end":2455},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"},{"id":"A2","pred":"image","subj":"T2","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"},{"id":"A3","pred":"image","subj":"T3","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G70323CJ"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T2","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"},{"subj":"T3","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":89,"end":98},"obj":"Body_part"},{"id":"T2","span":{"begin":239,"end":248},"obj":"Body_part"},{"id":"T3","span":{"begin":371,"end":380},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0007361"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0007361"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"A4","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000094"},{"id":"A5","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0000158"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T2","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T3","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":1938,"end":1942},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000560"},{"subj":"T1","pred":"source","obj":"CL-cell"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"uniprot-human","color":"#ab93ec","default":true},{"id":"GlycoBiology-GDGDB","color":"#94ec93"},{"id":"GlycoBiology-PACDB","color":"#ec93ae"},{"id":"GlycoBiology-FMA","color":"#93c8ec"},{"id":"uniprot-mouse","color":"#e2ec93"},{"id":"GlycoBiology-NCBITAXON","color":"#db93ec"},{"id":"sentences","color":"#93ecc1"},{"id":"GO-BP","color":"#eca793"},{"id":"GO-CC","color":"#9398ec"},{"id":"EDAM-topics","color":"#b2ec93"},{"id":"EDAM-DFO","color":"#ec93cc"},{"id":"NCBITAXON","color":"#93e6ec"},{"id":"GlyTouCan-IUPAC","color":"#ecd793"},{"id":"GlycoBiology-Motifs","color":"#bd93ec"},{"id":"Lectin","color":"#93eca3"},{"id":"Glycan-Motif","color":"#ec939c"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#93b6ec"},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#d0ec93"},{"id":"Anatomy-UBERON","color":"#ec93ea"},{"id":"CL-cell","color":"#93ecd4"}]}]}}