PubMed:1820198 / 447-670 JSONTXT

{"project":"sentences","denotations":[{"id":"TextSentencer_T3","span":{"begin":0,"end":223},"obj":"Sentence"},{"id":"T3","span":{"begin":0,"end":223},"obj":"Sentence"},{"id":"T3","span":{"begin":0,"end":223},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"The membrane-bound enzyme was solubilized with 0.5% Triton X-100 at a protein:detergent ratio of 2:1, and was then purified by ion-exchange chromatography on DEAE-cellulose, followed by hydroxyapatite column chromatography."}

{"project":"GlycoBiology-FMA","denotations":[{"id":"_T14","span":{"begin":70,"end":77},"obj":"FMAID:67257"},{"id":"_T15","span":{"begin":70,"end":77},"obj":"FMAID:165447"},{"id":"_T16","span":{"begin":163,"end":172},"obj":"FMAID:82748"},{"id":"_T17","span":{"begin":163,"end":172},"obj":"FMAID:196737"},{"id":"_T18","span":{"begin":201,"end":207},"obj":"FMAID:76737"},{"id":"_T19","span":{"begin":201,"end":207},"obj":"FMAID:183891"}],"namespaces":[{"prefix":"FMAID","uri":"http://purl.org/sig/ont/fma/fma"}],"text":"The membrane-bound enzyme was solubilized with 0.5% Triton X-100 at a protein:detergent ratio of 2:1, and was then purified by ion-exchange chromatography on DEAE-cellulose, followed by hydroxyapatite column chromatography."}

{"project":"GO-BP","denotations":[{"id":"T2","span":{"begin":131,"end":139},"obj":"http://purl.obolibrary.org/obo/GO_0015297"}],"text":"The membrane-bound enzyme was solubilized with 0.5% Triton X-100 at a protein:detergent ratio of 2:1, and was then purified by ion-exchange chromatography on DEAE-cellulose, followed by hydroxyapatite column chromatography."}

{"project":"GO-CC","denotations":[{"id":"T3","span":{"begin":4,"end":12},"obj":"http://purl.obolibrary.org/obo/GO_0016020"}],"text":"The membrane-bound enzyme was solubilized with 0.5% Triton X-100 at a protein:detergent ratio of 2:1, and was then purified by ion-exchange chromatography on DEAE-cellulose, followed by hydroxyapatite column chromatography."}

{"project":"EDAM-topics","denotations":[{"id":"T2","span":{"begin":70,"end":77},"obj":"http://edamontology.org/topic_0078"}],"text":"The membrane-bound enzyme was solubilized with 0.5% Triton X-100 at a protein:detergent ratio of 2:1, and was then purified by ion-exchange chromatography on DEAE-cellulose, followed by hydroxyapatite column chromatography."}

{"project":"EDAM-DFO","denotations":[{"id":"T3","span":{"begin":70,"end":77},"obj":"http://edamontology.org/data_1467"},{"id":"T4","span":{"begin":70,"end":77},"obj":"http://edamontology.org/format_1208"}],"text":"The membrane-bound enzyme was solubilized with 0.5% Triton X-100 at a protein:detergent ratio of 2:1, and was then purified by ion-exchange chromatography on DEAE-cellulose, followed by hydroxyapatite column chromatography."}

{"project":"GlycoBiology-Motifs","denotations":[{"id":"T1","span":{"begin":163,"end":172},"obj":"http://rdf.glycoinfo.org/glycan/G00025MO"}],"text":"The membrane-bound enzyme was solubilized with 0.5% Triton X-100 at a protein:detergent ratio of 2:1, and was then purified by ion-exchange chromatography on DEAE-cellulose, followed by hydroxyapatite column chromatography."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T4","span":{"begin":4,"end":12},"obj":"Body_part"}],"attributes":[{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"A5","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0000094"},{"id":"A6","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0000158"}],"text":"The membrane-bound enzyme was solubilized with 0.5% Triton X-100 at a protein:detergent ratio of 2:1, and was then purified by ion-exchange chromatography on DEAE-cellulose, followed by hydroxyapatite column chromatography."}