> top > docs > PubMed:18180300 > annotations

PubMed:18180300 JSON TXT

Annnotations TAB JSON ListView MergeView

PubMed_ArguminSci

Id	Subject	Object	Predicate	Lexical cue
T1	141-368	DRI_Challenge	denotes	Serum opacity factor (SOF) is a unique multifunctional virulence determinant expressed at the surface of Streptococcus pyogenes and has been shown to elicit protective immunity against GAS infection in a murine challenge model.
T2	369-560	DRI_Approach	denotes	SOF consists of two distinct domains with different binding capacities: an N-terminal domain that binds apolipoprotein AI and a C-terminal repeat domain that binds fibronectin and fibrinogen.
T3	561-707	DRI_Approach	denotes	The capacity of SOF to opacify serum by disrupting the structure of high density lipoproteins may preclude its use as a vaccine antigen in humans.
T4	708-856	DRI_Approach	denotes	This study generated mutant forms of recombinant SOF with reduced (100-fold) or abrogated opacity factor (OF) activity, for use as vaccine antigens.
T5	857-1077	DRI_Approach	denotes	However, alterations introduced into the N-terminal SOF peptide (SOFDeltaFn) by mutagenesis to abrogate OF activity, abolish the capacity of SOF to protect against lethal systemic S. pyogenes challenge in a murine model.
T6	1078-1242	DRI_Background	denotes	Mutant forms of purified SOFDeltaFn peptide were also used to assess the contribution of OF activity to the pathogenic processes of cell adhesion and cell invasion.
T7	1243-1451	DRI_Approach	denotes	Using latex beads coated with full-length SOF, SOFDeltaFn peptide, or a peptide encompassing the C-terminal repeats (FnBD), we demonstrate that adhesion to HEp-2 cells is mediated by both SOFDeltaFn and FnBD.
T8	1452-1552	DRI_Background	denotes	The HEp-2 cell binding displayed by the N-terminal SOFDeltaFn peptide is independent of OF activity.
T9	1553-1774	DRI_Outcome	denotes	We demonstrate that while the N terminus of SOF does not directly mediate intracellular uptake by epithelial cells, this domain enhances epithelial cell uptake mediated by full-length SOF, in comparison to the FnBD alone.

sentences

Id	Subject	Object	Predicate	Lexical cue
T1	0-140	Sentence	denotes	Opacity factor activity and epithelial cell binding by the serum opacity factor protein of Streptococcus pyogenes are functionally discrete.
T2	141-368	Sentence	denotes	Serum opacity factor (SOF) is a unique multifunctional virulence determinant expressed at the surface of Streptococcus pyogenes and has been shown to elicit protective immunity against GAS infection in a murine challenge model.
T3	369-560	Sentence	denotes	SOF consists of two distinct domains with different binding capacities: an N-terminal domain that binds apolipoprotein AI and a C-terminal repeat domain that binds fibronectin and fibrinogen.
T4	561-707	Sentence	denotes	The capacity of SOF to opacify serum by disrupting the structure of high density lipoproteins may preclude its use as a vaccine antigen in humans.
T5	708-856	Sentence	denotes	This study generated mutant forms of recombinant SOF with reduced (100-fold) or abrogated opacity factor (OF) activity, for use as vaccine antigens.
T6	857-1077	Sentence	denotes	However, alterations introduced into the N-terminal SOF peptide (SOFDeltaFn) by mutagenesis to abrogate OF activity, abolish the capacity of SOF to protect against lethal systemic S. pyogenes challenge in a murine model.
T7	1078-1242	Sentence	denotes	Mutant forms of purified SOFDeltaFn peptide were also used to assess the contribution of OF activity to the pathogenic processes of cell adhesion and cell invasion.
T8	1243-1451	Sentence	denotes	Using latex beads coated with full-length SOF, SOFDeltaFn peptide, or a peptide encompassing the C-terminal repeats (FnBD), we demonstrate that adhesion to HEp-2 cells is mediated by both SOFDeltaFn and FnBD.
T9	1452-1552	Sentence	denotes	The HEp-2 cell binding displayed by the N-terminal SOFDeltaFn peptide is independent of OF activity.
T10	1553-1774	Sentence	denotes	We demonstrate that while the N terminus of SOF does not directly mediate intracellular uptake by epithelial cells, this domain enhances epithelial cell uptake mediated by full-length SOF, in comparison to the FnBD alone.

mondo_disease

Id	Subject	Object	Predicate	Lexical cue	mondo_id
T1	330-339	Disease	denotes	infection	http://purl.obolibrary.org/obo/MONDO_0005550

NCBITAXON

Id	Subject	Object	Predicate	Lexical cue	db_id
T1	91-113	OrganismTaxon	denotes	Streptococcus pyogenes	1314
T2	246-268	OrganismTaxon	denotes	Streptococcus pyogenes	1314

Anatomy-UBERON

Id	Subject	Object	Predicate	Lexical cue	uberon_id
T1	28-43	Body_part	denotes	epithelial cell	http://purl.obolibrary.org/obo/CL_0000066
T2	1627-1640	Body_part	denotes	intracellular	http://purl.obolibrary.org/obo/GO_0005622
T3	1651-1667	Body_part	denotes	epithelial cells	http://purl.obolibrary.org/obo/CL_0000066
T4	1690-1705	Body_part	denotes	epithelial cell	http://purl.obolibrary.org/obo/CL_0000066

CL-cell

Id	Subject	Object	Predicate	Lexical cue	cl_id
T1	28-43	Cell	denotes	epithelial cell	http://purl.obolibrary.org/obo/CL:0000066
T2	1651-1667	Cell	denotes	epithelial cells	http://purl.obolibrary.org/obo/CL:0000066
T3	1690-1705	Cell	denotes	epithelial cell	http://purl.obolibrary.org/obo/CL:0000066