PubMed:18180292 JSONTXT

{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":89},"obj":"Sentence"},{"id":"T2","span":{"begin":90,"end":219},"obj":"Sentence"},{"id":"T3","span":{"begin":220,"end":419},"obj":"Sentence"},{"id":"T4","span":{"begin":420,"end":569},"obj":"Sentence"},{"id":"T5","span":{"begin":570,"end":734},"obj":"Sentence"},{"id":"T6","span":{"begin":735,"end":872},"obj":"Sentence"},{"id":"T7","span":{"begin":873,"end":975},"obj":"Sentence"},{"id":"T8","span":{"begin":976,"end":1155},"obj":"Sentence"},{"id":"T9","span":{"begin":1156,"end":1255},"obj":"Sentence"},{"id":"T10","span":{"begin":1256,"end":1437},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":89},"obj":"Sentence"},{"id":"T2","span":{"begin":90,"end":219},"obj":"Sentence"},{"id":"T3","span":{"begin":220,"end":419},"obj":"Sentence"},{"id":"T4","span":{"begin":420,"end":569},"obj":"Sentence"},{"id":"T5","span":{"begin":570,"end":734},"obj":"Sentence"},{"id":"T6","span":{"begin":735,"end":872},"obj":"Sentence"},{"id":"T7","span":{"begin":873,"end":975},"obj":"Sentence"},{"id":"T8","span":{"begin":976,"end":1155},"obj":"Sentence"},{"id":"T9","span":{"begin":1156,"end":1255},"obj":"Sentence"},{"id":"T10","span":{"begin":1256,"end":1437},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"c-Jun supports ribosomal RNA processing and nucleolar localization of RNA helicase DDX21.\nThe molecular mechanisms by which the AP-1 transcription factor c-Jun exerts its biological functions are not clearly understood. In addition to its well established role in transcriptional regulation of gene expression, several reports have suggested that c-Jun may also regulate cell behavior by non-transcriptional mechanisms. Here, we report that small interfering RNA-mediated depletion of c-Jun from mammalian cells results in inhibition of 28 S and 18 S rRNA accumulation. Moreover, we show that c-Jun depletion results in partial translocation of RNA helicase DDX21, implicated in rRNA processing, from the nucleolus to the nucleoplasm. We demonstrate that DDX21 translocation is rescued by exogenous c-Jun expression and that c-Jun depletion inhibits rRNA binding of DDX21. Furthermore, the direct interaction between c-Jun and DDX21 regulates nucleolar localization of DDX21. These results demonstrate that in addition to its transcriptional effects, c-Jun regulates rRNA processing and nucleolar compartmentalization of the rRNA processing protein DDX21. Thus, our results demonstrate a nucleolar mechanism through which c-Jun can regulate cell behavior. Moreover, these results suggest that the phenotypes observed previously in c-Jun-depleted mouse models and cell lines could be partly due to the effects of c-Jun on rRNA processing."}

{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":1346,"end":1351},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"10088"},{"id":"A2","pred":"db_id","subj":"T1","obj":"10090"}],"text":"c-Jun supports ribosomal RNA processing and nucleolar localization of RNA helicase DDX21.\nThe molecular mechanisms by which the AP-1 transcription factor c-Jun exerts its biological functions are not clearly understood. In addition to its well established role in transcriptional regulation of gene expression, several reports have suggested that c-Jun may also regulate cell behavior by non-transcriptional mechanisms. Here, we report that small interfering RNA-mediated depletion of c-Jun from mammalian cells results in inhibition of 28 S and 18 S rRNA accumulation. Moreover, we show that c-Jun depletion results in partial translocation of RNA helicase DDX21, implicated in rRNA processing, from the nucleolus to the nucleoplasm. We demonstrate that DDX21 translocation is rescued by exogenous c-Jun expression and that c-Jun depletion inhibits rRNA binding of DDX21. Furthermore, the direct interaction between c-Jun and DDX21 regulates nucleolar localization of DDX21. These results demonstrate that in addition to its transcriptional effects, c-Jun regulates rRNA processing and nucleolar compartmentalization of the rRNA processing protein DDX21. Thus, our results demonstrate a nucleolar mechanism through which c-Jun can regulate cell behavior. Moreover, these results suggest that the phenotypes observed previously in c-Jun-depleted mouse models and cell lines could be partly due to the effects of c-Jun on rRNA processing."}

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":15,"end":28},"obj":"Body_part"},{"id":"T2","span":{"begin":705,"end":714},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005840"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/GO_0005730"}],"text":"c-Jun supports ribosomal RNA processing and nucleolar localization of RNA helicase DDX21.\nThe molecular mechanisms by which the AP-1 transcription factor c-Jun exerts its biological functions are not clearly understood. In addition to its well established role in transcriptional regulation of gene expression, several reports have suggested that c-Jun may also regulate cell behavior by non-transcriptional mechanisms. Here, we report that small interfering RNA-mediated depletion of c-Jun from mammalian cells results in inhibition of 28 S and 18 S rRNA accumulation. Moreover, we show that c-Jun depletion results in partial translocation of RNA helicase DDX21, implicated in rRNA processing, from the nucleolus to the nucleoplasm. We demonstrate that DDX21 translocation is rescued by exogenous c-Jun expression and that c-Jun depletion inhibits rRNA binding of DDX21. Furthermore, the direct interaction between c-Jun and DDX21 regulates nucleolar localization of DDX21. These results demonstrate that in addition to its transcriptional effects, c-Jun regulates rRNA processing and nucleolar compartmentalization of the rRNA processing protein DDX21. Thus, our results demonstrate a nucleolar mechanism through which c-Jun can regulate cell behavior. Moreover, these results suggest that the phenotypes observed previously in c-Jun-depleted mouse models and cell lines could be partly due to the effects of c-Jun on rRNA processing."}