> top > docs > PubMed:18180291 > annotations

PubMed:18180291 JSONTXT

Annnotations TAB JSON ListView MergeView

sentences

Id Subject Object Predicate Lexical cue
T1 0-102 Sentence denotes AP-2-dependent internalization of potassium channel Kir2.3 is driven by a novel di-hydrophobic signal.
T2 103-272 Sentence denotes The localization and density of Kir2.3 channels are influenced by the balance between PDZ protein interaction at the cell surface and routing into the endocytic pathway.
T3 273-368 Sentence denotes Here, we explore mechanisms by which the Kir2.3 channel is directed into the endocytic pathway.
T4 369-527 Sentence denotes We found that Kir2.3 channels are constitutively internalized from the cell surface in a dynamin-dependent manner, indicative of vesicle-mediated endocytosis.
T5 528-784 Sentence denotes The rate of Kir2.3 endocytosis was dramatically attenuated following RNA interference-mediated knockdown of either alpha adaptin (AP-2 clathrin adaptor) or clathrin heavy chain, revealing that Kir2.3 is internalized by an AP-2 clathrin-dependent mechanism.
T6 785-1026 Sentence denotes Structure-rationalized mutagenesis studies of a number of different potential AP-2 interaction motifs indicate that internalization of Kir2.3 is largely dependent on a non-canonical di-isoleucine motif (II413) embedded within the C terminus.
T7 1027-1166 Sentence denotes Internalization assays using CD4-Kir2.3 chimeras demonstrate that the di-isoleucine signal acts in an autonomous and transplantable manner.
T8 1167-1305 Sentence denotes Kir2.3 co-immunoprecipitates with alpha adaptin, and disruption of the di-isoleucine motif decreased interaction of the channel with AP-2.
T9 1306-1432 Sentence denotes Replacement of the di-isoleucine motif with a canonical di-leucine internalization signal actually blocked Kir2.3 endocytosis.
T10 1433-1630 Sentence denotes Moreover, in yeast three-hybrid studies, the Kir2.3 di-isoleucine motif does not bind the AP-2 alphaC-sigma2 hemicomplex in the way that has been recently observed for canonical di-leucine signals.
T11 1631-1793 Sentence denotes Altogether, the results indicate that Kir2.3 channels are marked for clathrin-dependent internalization from the plasma membrane by a novel AP-2-dependent signal.
T1 0-102 Sentence denotes AP-2-dependent internalization of potassium channel Kir2.3 is driven by a novel di-hydrophobic signal.
T2 103-272 Sentence denotes The localization and density of Kir2.3 channels are influenced by the balance between PDZ protein interaction at the cell surface and routing into the endocytic pathway.
T3 273-368 Sentence denotes Here, we explore mechanisms by which the Kir2.3 channel is directed into the endocytic pathway.
T4 369-527 Sentence denotes We found that Kir2.3 channels are constitutively internalized from the cell surface in a dynamin-dependent manner, indicative of vesicle-mediated endocytosis.
T5 528-784 Sentence denotes The rate of Kir2.3 endocytosis was dramatically attenuated following RNA interference-mediated knockdown of either alpha adaptin (AP-2 clathrin adaptor) or clathrin heavy chain, revealing that Kir2.3 is internalized by an AP-2 clathrin-dependent mechanism.
T6 785-1026 Sentence denotes Structure-rationalized mutagenesis studies of a number of different potential AP-2 interaction motifs indicate that internalization of Kir2.3 is largely dependent on a non-canonical di-isoleucine motif (II413) embedded within the C terminus.
T7 1027-1166 Sentence denotes Internalization assays using CD4-Kir2.3 chimeras demonstrate that the di-isoleucine signal acts in an autonomous and transplantable manner.
T8 1167-1305 Sentence denotes Kir2.3 co-immunoprecipitates with alpha adaptin, and disruption of the di-isoleucine motif decreased interaction of the channel with AP-2.
T9 1306-1432 Sentence denotes Replacement of the di-isoleucine motif with a canonical di-leucine internalization signal actually blocked Kir2.3 endocytosis.
T10 1433-1630 Sentence denotes Moreover, in yeast three-hybrid studies, the Kir2.3 di-isoleucine motif does not bind the AP-2 alphaC-sigma2 hemicomplex in the way that has been recently observed for canonical di-leucine signals.
T11 1631-1793 Sentence denotes Altogether, the results indicate that Kir2.3 channels are marked for clathrin-dependent internalization from the plasma membrane by a novel AP-2-dependent signal.

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 498-505 Body_part denotes vesicle http://purl.obolibrary.org/obo/GO_0031982
T2 1744-1759 Body_part denotes plasma membrane http://purl.obolibrary.org/obo/GO_0005886