| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-107 |
Sentence |
denotes |
Palladin is an actin cross-linking protein that uses immunoglobulin-like domains to bind filamentous actin. |
| T2 |
108-215 |
Sentence |
denotes |
Palladin is a recently described phosphoprotein that plays an important role in cell adhesion and motility. |
| T3 |
216-341 |
Sentence |
denotes |
Previous studies have shown that palladin overexpression results in profound changes in actin organization in cultured cells. |
| T4 |
342-531 |
Sentence |
denotes |
Palladin binds to the actin-associated proteins alpha-actinin, vasodilator-stimulated phosphoprotein, profilin, Eps8, and ezrin, suggesting that it may affect actin organization indirectly. |
| T5 |
532-680 |
Sentence |
denotes |
To determine its molecular function in generating actin arrays, we purified palladin and asked if it is also capable of binding to F-actin directly. |
| T6 |
681-803 |
Sentence |
denotes |
In co-sedimentation and differential sedimentation assays, palladin was found to both bind and cross-link actin filaments. |
| T7 |
804-881 |
Sentence |
denotes |
This bundling activity was confirmed by fluorescence and electron microscopy. |
| T8 |
882-993 |
Sentence |
denotes |
Palladin fragments were then purified and used to determine the sequences necessary to bind and bundle F-actin. |
| T9 |
994-1182 |
Sentence |
denotes |
The Ig3 domain of palladin bound to F-actin, and a palladin fragment containing Ig3, Ig4, and the region linking these domains was identified as a fragment that was able to bundle F-actin. |
| T10 |
1183-1357 |
Sentence |
denotes |
Because palladin has multiple Ig domains, and only one of them binds to F-actin, this suggests that different Ig domains may be specialized for distinct biological functions. |
| T11 |
1358-1557 |
Sentence |
denotes |
In addition, our results suggest a potential role for palladin in generating specialized, actin-based cell morphologies via both direct actin cross-linking activity and indirect scaffolding activity. |
| T1 |
0-107 |
Sentence |
denotes |
Palladin is an actin cross-linking protein that uses immunoglobulin-like domains to bind filamentous actin. |
| T2 |
108-215 |
Sentence |
denotes |
Palladin is a recently described phosphoprotein that plays an important role in cell adhesion and motility. |
| T3 |
216-341 |
Sentence |
denotes |
Previous studies have shown that palladin overexpression results in profound changes in actin organization in cultured cells. |
| T4 |
342-531 |
Sentence |
denotes |
Palladin binds to the actin-associated proteins alpha-actinin, vasodilator-stimulated phosphoprotein, profilin, Eps8, and ezrin, suggesting that it may affect actin organization indirectly. |
| T5 |
532-680 |
Sentence |
denotes |
To determine its molecular function in generating actin arrays, we purified palladin and asked if it is also capable of binding to F-actin directly. |
| T6 |
681-803 |
Sentence |
denotes |
In co-sedimentation and differential sedimentation assays, palladin was found to both bind and cross-link actin filaments. |
| T7 |
804-881 |
Sentence |
denotes |
This bundling activity was confirmed by fluorescence and electron microscopy. |
| T8 |
882-993 |
Sentence |
denotes |
Palladin fragments were then purified and used to determine the sequences necessary to bind and bundle F-actin. |
| T9 |
994-1182 |
Sentence |
denotes |
The Ig3 domain of palladin bound to F-actin, and a palladin fragment containing Ig3, Ig4, and the region linking these domains was identified as a fragment that was able to bundle F-actin. |
| T10 |
1183-1357 |
Sentence |
denotes |
Because palladin has multiple Ig domains, and only one of them binds to F-actin, this suggests that different Ig domains may be specialized for distinct biological functions. |
| T11 |
1358-1557 |
Sentence |
denotes |
In addition, our results suggest a potential role for palladin in generating specialized, actin-based cell morphologies via both direct actin cross-linking activity and indirect scaffolding activity. |