| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T8 |
685-751 |
Sentence |
denotes |
Expression of chemokine receptors was determined by FACS analysis. |
| TextSentencer_T1 |
0-99 |
Sentence |
denotes |
15-Lipoxygenase-2 expression in human macrophages induces chemokine secretion and T cell migration. |
| TextSentencer_T2 |
100-111 |
Sentence |
denotes |
BACKGROUND: |
| TextSentencer_T3 |
112-251 |
Sentence |
denotes |
We determined previously that hypoxia results in increased 15-lipoxygenase type 2 (15-LOX-2) expression and CXCL8 secretion in macrophages. |
| TextSentencer_T4 |
252-451 |
Sentence |
denotes |
This study sought to determine whether 15-LOX-2 expression links directly with the secretion of inflammatory molecules in macrophages and also investigated its subsequent effects on T cell migration. |
| TextSentencer_T5 |
452-460 |
Sentence |
denotes |
METHODS: |
| TextSentencer_T6 |
461-550 |
Sentence |
denotes |
Adenovirus-mediated gene delivery caused overexpression of 15-LOX-2 in human macrophages. |
| TextSentencer_T7 |
551-684 |
Sentence |
denotes |
We used cytometric bead array to measure chemokine secretion, and assessed T cell migration by counting cells in chemotaxis chambers. |
| TextSentencer_T9 |
752-817 |
Sentence |
denotes |
Using siRNA, we reduced 15-LOX-2 expression in human macrophages. |
| TextSentencer_T10 |
818-853 |
Sentence |
denotes |
We used scrambled siRNA as control. |
| TextSentencer_T11 |
854-862 |
Sentence |
denotes |
RESULTS: |
| TextSentencer_T12 |
863-969 |
Sentence |
denotes |
Macrophages that overexpress 15-LOX-2 showed increased secretion of chemokine CXCL10 after 24h incubation. |
| TextSentencer_T13 |
970-1120 |
Sentence |
denotes |
In addition, preconditioned medium from 15-LOX-2-overexpressing cells increased T cell migration and surface expression of CXCR3, the CXCL10 receptor. |
| TextSentencer_T14 |
1121-1240 |
Sentence |
denotes |
Knockdown of 15-LOX-2 expression decreased CXCL10 secretion from hypoxic macrophages and also reduced T cell migration. |
| TextSentencer_T15 |
1241-1252 |
Sentence |
denotes |
CONCLUSION: |
| TextSentencer_T16 |
1253-1346 |
Sentence |
denotes |
In macrophages, overexpression of 15-LOX-2 results in increased secretion of CXCL10 and CCL2. |
| TextSentencer_T17 |
1347-1515 |
Sentence |
denotes |
Products released in response to increased 15-LOX-2 activation lead to increased expression of CD69, the T cell activation marker as well as increased T cell migration. |
| TextSentencer_T18 |
1516-1653 |
Sentence |
denotes |
Therefore, increased expression of 15-LOX-2 induced by hypoxia may participate in T cell recruitment in diseases such as atherosclerosis. |
| T1 |
0-99 |
Sentence |
denotes |
15-Lipoxygenase-2 expression in human macrophages induces chemokine secretion and T cell migration. |
| T2 |
100-111 |
Sentence |
denotes |
BACKGROUND: |
| T3 |
112-251 |
Sentence |
denotes |
We determined previously that hypoxia results in increased 15-lipoxygenase type 2 (15-LOX-2) expression and CXCL8 secretion in macrophages. |
| T4 |
252-451 |
Sentence |
denotes |
This study sought to determine whether 15-LOX-2 expression links directly with the secretion of inflammatory molecules in macrophages and also investigated its subsequent effects on T cell migration. |
| T5 |
452-460 |
Sentence |
denotes |
METHODS: |
| T6 |
461-550 |
Sentence |
denotes |
Adenovirus-mediated gene delivery caused overexpression of 15-LOX-2 in human macrophages. |
| T7 |
551-684 |
Sentence |
denotes |
We used cytometric bead array to measure chemokine secretion, and assessed T cell migration by counting cells in chemotaxis chambers. |
| T8 |
685-751 |
Sentence |
denotes |
Expression of chemokine receptors was determined by FACS analysis. |
| T9 |
752-817 |
Sentence |
denotes |
Using siRNA, we reduced 15-LOX-2 expression in human macrophages. |
| T10 |
818-853 |
Sentence |
denotes |
We used scrambled siRNA as control. |
| T11 |
854-862 |
Sentence |
denotes |
RESULTS: |
| T12 |
863-969 |
Sentence |
denotes |
Macrophages that overexpress 15-LOX-2 showed increased secretion of chemokine CXCL10 after 24h incubation. |
| T13 |
970-1120 |
Sentence |
denotes |
In addition, preconditioned medium from 15-LOX-2-overexpressing cells increased T cell migration and surface expression of CXCR3, the CXCL10 receptor. |
| T14 |
1121-1240 |
Sentence |
denotes |
Knockdown of 15-LOX-2 expression decreased CXCL10 secretion from hypoxic macrophages and also reduced T cell migration. |
| T15 |
1241-1252 |
Sentence |
denotes |
CONCLUSION: |
| T16 |
1253-1346 |
Sentence |
denotes |
In macrophages, overexpression of 15-LOX-2 results in increased secretion of CXCL10 and CCL2. |
| T17 |
1347-1515 |
Sentence |
denotes |
Products released in response to increased 15-LOX-2 activation lead to increased expression of CD69, the T cell activation marker as well as increased T cell migration. |
| T18 |
1516-1653 |
Sentence |
denotes |
Therefore, increased expression of 15-LOX-2 induced by hypoxia may participate in T cell recruitment in diseases such as atherosclerosis. |