Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
101-208 |
DRI_Background |
denotes |
Very little is known about SET- and MYND-containing protein 2 (SMYD2), a member of the SMYD protein family. |
T2 |
209-362 |
DRI_Background |
denotes |
However, the interest in better understanding the roles of SMYD2 has grown because of recent reports indicating that SMYD2 methylates p53 and histone H3. |
T3 |
363-481 |
DRI_Outcome |
denotes |
In this study, we present a combined proteomics and genomics study of SMYD2 designed to elucidate its molecular roles. |
T4 |
482-713 |
DRI_Outcome |
denotes |
We report the cytosolic and nuclear interactome of SMYD2 using a combination of immunoprecipitation coupled with high throughput MS, chromatin immunoprecipitation coupled with high throughput MS, and co-immunoprecipitation methods. |
T5 |
714-894 |
DRI_Approach |
denotes |
In particular, we report that SMYD2 interacted with HSP90alpha independently of the SET and MYND domains, with EBP41L3 through the MYND domain, and with p53 through the SET domain. |
T6 |
895-1066 |
DRI_Outcome |
denotes |
We demonstrated that the interaction of SMYD2 with HSP90alpha enhances SMYD2 histone methyltransferase activity and specificity for histone H3 at lysine 4 (H3K4) in vitro. |
T7 |
1067-1225 |
DRI_Background |
denotes |
Interestingly histone H3K36 methyltransferase activity was independent of its interaction with HSP90alpha similar to LSD1 dependence on the androgen receptor. |
T8 |
1226-1301 |
DRI_Approach |
denotes |
We also showed that the SET domain is required for the methylation at H3K4. |
T9 |
1302-1508 |
DRI_Outcome |
denotes |
We demonstrated using a modified chromatin immunoprecipitation protocol that the SMYD2 gain of function leads to an increase in H3K4 methylation in vivo, whereas no observable levels of H3K36 were detected. |
T10 |
1509-1742 |
DRI_Outcome |
denotes |
We also report that the SMYD2 gain of function was correlated with the up-regulation of 37 and down-regulation of four genes, the majority of which are involved in the cell cycle, chromatin remodeling, and transcriptional regulation. |
T11 |
1743-1820 |
DRI_Background |
denotes |
TACC2 is one of the genes up-regulated as a result of SMYD2 gain of function. |
T12 |
1821-1938 |
DRI_Background |
denotes |
Up-regulation of TACC2 by SMYD2 occurred as a result of SMYD2 binding to the TACC2 promoter where it methylates H3K4. |
T13 |
1939-1973 |
DRI_Outcome |
denotes |
Furthermore the combination of the |
T14 |
1992-2128 |
DRI_Outcome |
denotes |
with the gene expression data suggests that some of the genes regulated by SMYD2 are closely associated with SMYD2-interacting proteins. |