PubMed:1804535 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1804535","sourcedb":"PubMed","sourceid":"1804535","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1804535","text":"A method for the solubilization of a (1----3)-beta-D-glucan isolated from Saccharomyces cerevisiae.\nThis report describes a method for the solubilization of a micro-particulate beta-D-glucan. Insoluble glucan is dissolved in methyl sulfoxide and urea (8M) and partially phosphorylated at 100 degrees. The resulting water-soluble product is called glucan phosphate. The conversion rate is 70%, and the preparation is endotoxin free as determined by the Limulus lysate procedure. Glucan phosphate is composed of 34.66% C, 6.29% H, 42.83% O, and 2.23% P and has a repeating-unit empirical formula of (C6H10O5)7.PO3H2, indicating a phosphate group substitution on every seventh glucose subunit. Molecular-weight averages, polydispersity, and intrinsic viscosity were determined by aqueous high-performance size-exclusion chromatography (s.e.c.) with on-line, multi-angle laser light scattering (m.a.l.l.s.) photometry and differential viscometry (d.v.). Two polymer peaks were resolved. Peak 1 (Mw = 3.57 x 10(6) daltons), represents approximately 2% of the total polymers, while peak 2 (Mw = 1.10 x 10(5) daltons) comprises approximately 98% of polymers. 13C- and 31P-n.m.r. spectroscopy confirmed the beta-1,3 interchain linkage and the presence of a phosphate group. In solution, glucan phosphate polymers self-associate in a triple-helical arrangement. The ability to prepare a immunologically active, non-toxic, water-soluble beta-D-glucan preparation will greatly enhance the clinical utility of this class of compounds.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":99},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":100,"end":191},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":192,"end":300},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":301,"end":364},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":365,"end":477},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":478,"end":690},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":691,"end":949},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":950,"end":982},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":983,"end":1151},"obj":"Sentence"},{"id":"TextSentencer_T10","span":{"begin":1152,"end":1265},"obj":"Sentence"},{"id":"TextSentencer_T11","span":{"begin":1266,"end":1352},"obj":"Sentence"},{"id":"TextSentencer_T12","span":{"begin":1353,"end":1522},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":99},"obj":"Sentence"},{"id":"T2","span":{"begin":100,"end":191},"obj":"Sentence"},{"id":"T3","span":{"begin":192,"end":300},"obj":"Sentence"},{"id":"T4","span":{"begin":301,"end":364},"obj":"Sentence"},{"id":"T5","span":{"begin":365,"end":477},"obj":"Sentence"},{"id":"T6","span":{"begin":478,"end":690},"obj":"Sentence"},{"id":"T7","span":{"begin":691,"end":949},"obj":"Sentence"},{"id":"T8","span":{"begin":950,"end":982},"obj":"Sentence"},{"id":"T9","span":{"begin":983,"end":1151},"obj":"Sentence"},{"id":"T10","span":{"begin":1152,"end":1265},"obj":"Sentence"},{"id":"T11","span":{"begin":1266,"end":1352},"obj":"Sentence"},{"id":"T12","span":{"begin":1353,"end":1522},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T10","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T11","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T12","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"},{"subj":"T10","pred":"source","obj":"sentences"},{"subj":"T11","pred":"source","obj":"sentences"},{"subj":"T12","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":74,"end":98},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":452,"end":459},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"4932"},{"id":"A2","pred":"db_id","subj":"T2","obj":"6849"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":674,"end":681},"obj":"https://glytoucan.org/Structures/Glycans/G15021LG"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":674,"end":681},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G15021LG"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#ecc993","default":true},{"id":"NCBITAXON","color":"#af93ec"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#93ec95"},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#ec93aa"}]}]}}