PubMed:1802386 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1802386","sourcedb":"PubMed","sourceid":"1802386","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1802386","text":"Structural characterization of two oligosaccharide fragments formed by the selective cleavage of rhamnogalacturonan II: evidence for the anomeric configuration and attachment sites of apiose and 3-deoxy-2-heptulosaric acid.\nEvidence for the anomeric configurations and attachment sites of 3-deoxy-D-lyxo-2-heptulosaric acid (DHA) and apiosyl residues has been obtained through the characterization of two oligoglycosyl fragments isolated from rhamnogalacturonan II (RG-II). One of the oligoglycosyl fragments, a pentaglycosyl aldonic acid generated by Smith degradation of RG-II, was composed of four D-galactopyranosyluronic acid residues, a DHA residue, and a threonic acid residue (derived from a D-galactopyranosyluronic acid residue). The structural analysis of the pentaglycosyl aldonic acid established the beta-D-configuration for the DHA residue. Furthermore, it established that a previously identified diglycosyl side chain, 5-O-(beta-L-arabinofuranosyl)-DHA was directly attached to O-3 of a D-galactopyranosyluronic acid residue in the backbone of RG-II. The second oligoglycosyl fragment, a peralkylated diglycosyl hex-1-enitol, was generated by hex-5-enose degradation of permethylated and carboxyl-reduced RG-II. The structure of the peralkylated diglycosyl hex-1-enitol, beta-L-Rhap-(1----3')-beta-D-Apif-(1----5)-hex-1-enitol++ +, was determined by a combination of glycosyl-linkage composition analysis and n.m.r. spectroscopy. The n.m.r. data indicated the beta-configuration for the D-apiosyl residue. The isolation and characterization of the diglycosyl hex-1-enitol also established that a previously identified heptaglycosyl side chain was directly attached to O-2 of a D-galactopyranosyluronic acid in the backbone of RG-II.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":223},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":224,"end":473},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":474,"end":739},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":740,"end":855},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":856,"end":1067},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":1068,"end":1228},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1229,"end":1446},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1447,"end":1522},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1523,"end":1749},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":223},"obj":"Sentence"},{"id":"T2","span":{"begin":224,"end":473},"obj":"Sentence"},{"id":"T3","span":{"begin":474,"end":739},"obj":"Sentence"},{"id":"T4","span":{"begin":740,"end":855},"obj":"Sentence"},{"id":"T5","span":{"begin":856,"end":1067},"obj":"Sentence"},{"id":"T6","span":{"begin":1068,"end":1228},"obj":"Sentence"},{"id":"T7","span":{"begin":1229,"end":1446},"obj":"Sentence"},{"id":"T8","span":{"begin":1447,"end":1522},"obj":"Sentence"},{"id":"T9","span":{"begin":1523,"end":1749},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":164,"end":180},"obj":"Body_part"},{"id":"T2","span":{"begin":269,"end":285},"obj":"Body_part"},{"id":"T3","span":{"begin":1049,"end":1057},"obj":"Body_part"},{"id":"T4","span":{"begin":1731,"end":1739},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_4200047"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_4200047"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0001130"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0001130"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T2","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T3","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T4","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#93ecd1","default":true},{"id":"Anatomy-UBERON","color":"#ecb793"}]}]}}