Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-116 |
Sentence |
denotes |
ADRB3 adrenergic receptor is a key regulator of human myometrial apoptosis and inflammation during chorioamnionitis. |
T2 |
117-248 |
Sentence |
denotes |
The pathophysiology underlying preterm labor triggered by inflammatory conditions such as chorioamnionitis remains largely unclear. |
T3 |
249-391 |
Sentence |
denotes |
It has already been suggested that beta-3 adrenergic (ADRB3) agonists might be of interest in the pharmacological management of preterm labor. |
T4 |
392-532 |
Sentence |
denotes |
Although there is evidence implicating ADRB receptors in the control of inflammation, there are minimal data relating specifically to ADRB3. |
T5 |
533-755 |
Sentence |
denotes |
To explore the cellular consequences of chorioamnionitis and detect apoptosis, we first performed immunostaining and Western blot experiments on human myometrial samples obtained from women with confirmed chorioamnionitis. |
T6 |
756-936 |
Sentence |
denotes |
We then developed an in vitro model of chorioamnionitis by incubating the myometrial samples obtained from uncomplicated pregnancies with Escherichia coli lipopolysaccharide (LPS). |
T7 |
937-1163 |
Sentence |
denotes |
We observed that chorioamnionitis was associated with a significant increase in cleaved CASP3 protein expression, as well as chromatin condensation, which were reproduced experimentally by LPS stimulation (10 microg/ml, 48 h). |
T8 |
1164-1337 |
Sentence |
denotes |
Lipopolysaccharide stimulation of normal human myometrium also induced CASP3 transcripts, increased the proapoptotic marker BAX, and decreased the antiapoptotic marker BCL2. |
T9 |
1338-1466 |
Sentence |
denotes |
Lipopolysaccharide-induced apoptosis was antagonized by neutralization of secreted tumor necrosis factor by a specific antibody. |
T10 |
1467-1585 |
Sentence |
denotes |
Furthermore, LPS stimulation increased medium culture levels of proinflammatory cytokines interleukin 6 (IL6) and IL8. |
T11 |
1586-1744 |
Sentence |
denotes |
Lipopolysaccharide-induced apoptosis and cytokine production were prevented by the new and potent ADRB3 agonist SAR150640 in a concentration-dependent manner. |
T12 |
1745-1847 |
Sentence |
denotes |
SAR150640 by itself did not exhibit any effect on apoptosis or cytokine production in control tissues. |
T13 |
1848-1942 |
Sentence |
denotes |
This study shows that chorioamnionitis is associated with apoptosis of human myometrial cells. |
T14 |
1943-2074 |
Sentence |
denotes |
It emphasizes the potential therapeutic interest of ADRB3 agonists in the field of preterm labor and other inflammatory conditions. |
T1 |
0-116 |
Sentence |
denotes |
ADRB3 adrenergic receptor is a key regulator of human myometrial apoptosis and inflammation during chorioamnionitis. |
T2 |
117-248 |
Sentence |
denotes |
The pathophysiology underlying preterm labor triggered by inflammatory conditions such as chorioamnionitis remains largely unclear. |
T3 |
249-391 |
Sentence |
denotes |
It has already been suggested that beta-3 adrenergic (ADRB3) agonists might be of interest in the pharmacological management of preterm labor. |
T4 |
392-532 |
Sentence |
denotes |
Although there is evidence implicating ADRB receptors in the control of inflammation, there are minimal data relating specifically to ADRB3. |
T5 |
533-755 |
Sentence |
denotes |
To explore the cellular consequences of chorioamnionitis and detect apoptosis, we first performed immunostaining and Western blot experiments on human myometrial samples obtained from women with confirmed chorioamnionitis. |
T6 |
756-936 |
Sentence |
denotes |
We then developed an in vitro model of chorioamnionitis by incubating the myometrial samples obtained from uncomplicated pregnancies with Escherichia coli lipopolysaccharide (LPS). |
T7 |
937-1163 |
Sentence |
denotes |
We observed that chorioamnionitis was associated with a significant increase in cleaved CASP3 protein expression, as well as chromatin condensation, which were reproduced experimentally by LPS stimulation (10 microg/ml, 48 h). |
T8 |
1164-1337 |
Sentence |
denotes |
Lipopolysaccharide stimulation of normal human myometrium also induced CASP3 transcripts, increased the proapoptotic marker BAX, and decreased the antiapoptotic marker BCL2. |
T9 |
1338-1466 |
Sentence |
denotes |
Lipopolysaccharide-induced apoptosis was antagonized by neutralization of secreted tumor necrosis factor by a specific antibody. |
T10 |
1467-1585 |
Sentence |
denotes |
Furthermore, LPS stimulation increased medium culture levels of proinflammatory cytokines interleukin 6 (IL6) and IL8. |
T11 |
1586-1744 |
Sentence |
denotes |
Lipopolysaccharide-induced apoptosis and cytokine production were prevented by the new and potent ADRB3 agonist SAR150640 in a concentration-dependent manner. |
T12 |
1745-1847 |
Sentence |
denotes |
SAR150640 by itself did not exhibit any effect on apoptosis or cytokine production in control tissues. |
T13 |
1848-1942 |
Sentence |
denotes |
This study shows that chorioamnionitis is associated with apoptosis of human myometrial cells. |
T14 |
1943-2074 |
Sentence |
denotes |
It emphasizes the potential therapeutic interest of ADRB3 agonists in the field of preterm labor and other inflammatory conditions. |