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PubMed_Structured_Abstracts

Id Subject Object Predicate Lexical cue
T1 108-543 BACKGROUND denotes The molecular mechanisms involved in breast cancer metastasis still remain unclear to date. In our previous study, differential expression of peroxiredoxin 6 was found between the highly metastatic MDA-MB-435HM cells and their parental counterparts, MDA-MB-435 cells. In this study, we investigated the effects of peroxiredoxin 6 on the proliferation and metastatic potential of human breast cancer cells and their potential mechanism.
T2 553-1403 METHODS denotes Expression of peroxiredoxin 6 in the highly metastatic MDA-MB-231HM cells was investigated by RT-PCR, real-time PCR and western blot. A recombinant expression plasmid of the human peroxiredoxin 6 gene was constructed and transfected into MDA-MB-231 and MDA-MB-435 cells. The effects of peroxiredoxin 6 on the proliferation and invasion of MDA-MB-231 and MDA-MB-435 cells were investigated by the Cell Counting Kit-8 method, colony-formation assay, adhesion assay, flow cytometry and invasion assay in vitro. miRNA was used to downregulate the expression of peroxiredoxin 6. Genes related to the invasion and metastasis of cancer were determined by RT-PCR, real-time PCR and western blot. The tumorigenicity and spontaneously metastatic capability regulated by peroxiredoxin 6 were determined using an orthotopic xenograft tumor model in athymic mice.
T3 1413-2471 RESULTS denotes Overexpression of peroxiredoxin 6 in MDA-MB-231HM cells compared with their parental counterparts was confirmed. Upregulation of peroxiredoxin 6 enhanced the in vitro proliferation and invasion of breast cancer cells. The enhancement was associated with decreasing levels of tissue inhibitor of matrix metalloproteinase (TIMP)-2 and increasing levels of the urokinase-type plasminogen activator receptor (uPAR), Ets-1 (E26 transformation-specific-1), matrix metalloproteinase (MMP)-9 and RhoC (ras homolog gene family, member C) expression. The results were further demonstrated by RNA interference experiments in vitro. In an in vivo study, we also demonstrated that peroxiredoxin 6-transfected breast cancer cells grew much faster and had more pulmonary metastases than control cells. By contrast, peroxiredoxin 6 knockdown breast cancer cells grew more slowly and had fewer pulmonary metastases. Effects similar to those of peroxiredoxin 6 on the uPAR, Ets-1, MMP-9, RhoC and TIMP-2 expression observed in in vitro studies were found in the in vivo study.
T4 2484-2703 CONCLUSIONS denotes Overexpression of peroxiredoxin 6 leads to a more invasive phenotype and metastatic potential in human breast cancer, at least in part, through regulation of the levels of uPAR, Ets-1, MMP-9, RhoC and TIMP-2 expression.

PMID_GLOBAL

Id Subject Object Predicate Lexical cue mondo_id
T1 79-92 DiseaseOrPhenotypicFeature denotes breast cancer 0004989|0007254
T3 145-158 DiseaseOrPhenotypicFeature denotes breast cancer 0004989|0007254
T5 493-506 DiseaseOrPhenotypicFeature denotes breast cancer 0004989|0007254
T7 1375-1380 DiseaseOrPhenotypicFeature denotes tumor 0005070
T8 1610-1623 DiseaseOrPhenotypicFeature denotes breast cancer 0004989|0007254
T10 2109-2122 DiseaseOrPhenotypicFeature denotes breast cancer 0004989|0007254
T12 2239-2252 DiseaseOrPhenotypicFeature denotes breast cancer 0004989|0007254
T14 2587-2600 DiseaseOrPhenotypicFeature denotes breast cancer 0004989|0007254

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-93 Sentence denotes Identification of the functional role of peroxiredoxin 6 in the progression of breast cancer.
TextSentencer_T2 94-107 Sentence denotes INTRODUCTION:
TextSentencer_T3 108-199 Sentence denotes The molecular mechanisms involved in breast cancer metastasis still remain unclear to date.
TextSentencer_T4 200-375 Sentence denotes In our previous study, differential expression of peroxiredoxin 6 was found between the highly metastatic MDA-MB-435HM cells and their parental counterparts, MDA-MB-435 cells.
TextSentencer_T5 376-543 Sentence denotes In this study, we investigated the effects of peroxiredoxin 6 on the proliferation and metastatic potential of human breast cancer cells and their potential mechanism.
TextSentencer_T6 544-552 Sentence denotes METHODS:
TextSentencer_T7 553-686 Sentence denotes Expression of peroxiredoxin 6 in the highly metastatic MDA-MB-231HM cells was investigated by RT-PCR, real-time PCR and western blot.
TextSentencer_T8 687-823 Sentence denotes A recombinant expression plasmid of the human peroxiredoxin 6 gene was constructed and transfected into MDA-MB-231 and MDA-MB-435 cells.
TextSentencer_T9 824-1126 Sentence denotes The effects of peroxiredoxin 6 on the proliferation and invasion of MDA-MB-231 and MDA-MB-435 cells were investigated by the Cell Counting Kit-8 method, colony-formation assay, adhesion assay, flow cytometry and invasion assay in vitro. miRNA was used to downregulate the expression of peroxiredoxin 6.
TextSentencer_T10 1127-1240 Sentence denotes Genes related to the invasion and metastasis of cancer were determined by RT-PCR, real-time PCR and western blot.
TextSentencer_T11 1241-1403 Sentence denotes The tumorigenicity and spontaneously metastatic capability regulated by peroxiredoxin 6 were determined using an orthotopic xenograft tumor model in athymic mice.
TextSentencer_T12 1404-1412 Sentence denotes RESULTS:
TextSentencer_T13 1413-1525 Sentence denotes Overexpression of peroxiredoxin 6 in MDA-MB-231HM cells compared with their parental counterparts was confirmed.
TextSentencer_T14 1526-1630 Sentence denotes Upregulation of peroxiredoxin 6 enhanced the in vitro proliferation and invasion of breast cancer cells.
TextSentencer_T15 1631-1953 Sentence denotes The enhancement was associated with decreasing levels of tissue inhibitor of matrix metalloproteinase (TIMP)-2 and increasing levels of the urokinase-type plasminogen activator receptor (uPAR), Ets-1 (E26 transformation-specific-1), matrix metalloproteinase (MMP)-9 and RhoC (ras homolog gene family, member C) expression.
TextSentencer_T16 1954-2033 Sentence denotes The results were further demonstrated by RNA interference experiments in vitro.
TextSentencer_T17 2034-2199 Sentence denotes In an in vivo study, we also demonstrated that peroxiredoxin 6-transfected breast cancer cells grew much faster and had more pulmonary metastases than control cells.
TextSentencer_T18 2200-2311 Sentence denotes By contrast, peroxiredoxin 6 knockdown breast cancer cells grew more slowly and had fewer pulmonary metastases.
TextSentencer_T19 2312-2471 Sentence denotes Effects similar to those of peroxiredoxin 6 on the uPAR, Ets-1, MMP-9, RhoC and TIMP-2 expression observed in in vitro studies were found in the in vivo study.
TextSentencer_T20 2472-2483 Sentence denotes CONCLUSION:
TextSentencer_T21 2484-2703 Sentence denotes Overexpression of peroxiredoxin 6 leads to a more invasive phenotype and metastatic potential in human breast cancer, at least in part, through regulation of the levels of uPAR, Ets-1, MMP-9, RhoC and TIMP-2 expression.
T1 0-93 Sentence denotes Identification of the functional role of peroxiredoxin 6 in the progression of breast cancer.
T2 94-107 Sentence denotes INTRODUCTION:
T3 108-199 Sentence denotes The molecular mechanisms involved in breast cancer metastasis still remain unclear to date.
T4 200-375 Sentence denotes In our previous study, differential expression of peroxiredoxin 6 was found between the highly metastatic MDA-MB-435HM cells and their parental counterparts, MDA-MB-435 cells.
T5 376-543 Sentence denotes In this study, we investigated the effects of peroxiredoxin 6 on the proliferation and metastatic potential of human breast cancer cells and their potential mechanism.
T6 544-552 Sentence denotes METHODS:
T7 553-686 Sentence denotes Expression of peroxiredoxin 6 in the highly metastatic MDA-MB-231HM cells was investigated by RT-PCR, real-time PCR and western blot.
T8 687-823 Sentence denotes A recombinant expression plasmid of the human peroxiredoxin 6 gene was constructed and transfected into MDA-MB-231 and MDA-MB-435 cells.
T9 824-1126 Sentence denotes The effects of peroxiredoxin 6 on the proliferation and invasion of MDA-MB-231 and MDA-MB-435 cells were investigated by the Cell Counting Kit-8 method, colony-formation assay, adhesion assay, flow cytometry and invasion assay in vitro. miRNA was used to downregulate the expression of peroxiredoxin 6.
T10 1127-1240 Sentence denotes Genes related to the invasion and metastasis of cancer were determined by RT-PCR, real-time PCR and western blot.
T11 1241-1403 Sentence denotes The tumorigenicity and spontaneously metastatic capability regulated by peroxiredoxin 6 were determined using an orthotopic xenograft tumor model in athymic mice.
T12 1404-1412 Sentence denotes RESULTS:
T13 1413-1525 Sentence denotes Overexpression of peroxiredoxin 6 in MDA-MB-231HM cells compared with their parental counterparts was confirmed.
T14 1526-1630 Sentence denotes Upregulation of peroxiredoxin 6 enhanced the in vitro proliferation and invasion of breast cancer cells.
T15 1631-1953 Sentence denotes The enhancement was associated with decreasing levels of tissue inhibitor of matrix metalloproteinase (TIMP)-2 and increasing levels of the urokinase-type plasminogen activator receptor (uPAR), Ets-1 (E26 transformation-specific-1), matrix metalloproteinase (MMP)-9 and RhoC (ras homolog gene family, member C) expression.
T16 1954-2033 Sentence denotes The results were further demonstrated by RNA interference experiments in vitro.
T17 2034-2199 Sentence denotes In an in vivo study, we also demonstrated that peroxiredoxin 6-transfected breast cancer cells grew much faster and had more pulmonary metastases than control cells.
T18 2200-2311 Sentence denotes By contrast, peroxiredoxin 6 knockdown breast cancer cells grew more slowly and had fewer pulmonary metastases.
T19 2312-2471 Sentence denotes Effects similar to those of peroxiredoxin 6 on the uPAR, Ets-1, MMP-9, RhoC and TIMP-2 expression observed in in vitro studies were found in the in vivo study.
T20 2472-2483 Sentence denotes CONCLUSION:
T21 2484-2703 Sentence denotes Overexpression of peroxiredoxin 6 leads to a more invasive phenotype and metastatic potential in human breast cancer, at least in part, through regulation of the levels of uPAR, Ets-1, MMP-9, RhoC and TIMP-2 expression.

DisGeNET

Id Subject Object Predicate Lexical cue
T0 2502-2517 gene:9588 denotes peroxiredoxin 6
T1 2587-2600 disease:C0006142 denotes breast cancer
T2 2502-2517 gene:9588 denotes peroxiredoxin 6
T3 2587-2600 disease:C0678222 denotes breast cancer
R1 T0 T1 associated_with peroxiredoxin 6,breast cancer
R2 T2 T3 associated_with peroxiredoxin 6,breast cancer

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 133-151 HP_0000769 denotes involved in breast
T2 145-158 HP_0003002 denotes breast cancer
T3 145-158 HP_0100013 denotes breast cancer
T4 152-158 HP_0002664 denotes cancer
T5 493-506 HP_0003002 denotes breast cancer
T6 493-506 HP_0100013 denotes breast cancer
T7 500-506 HP_0002664 denotes cancer

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
17980029-0#41#56#gene9588 1110-1125 gene9588 denotes peroxiredoxin 6
17980029-0#79#92#diseaseC0006142 1610-1623 diseaseC0006142 denotes breast cancer
17980029-0#79#92#diseaseC0678222 1610-1623 diseaseC0678222 denotes breast cancer
17980029-14#13#28#gene9588 2213-2228 gene9588 denotes peroxiredoxin 6
17980029-14#90#110#diseaseC0153676 2290-2310 diseaseC0153676 denotes pulmonary metastases
41#56#gene958879#92#diseaseC0006142 17980029-0#41#56#gene9588 17980029-0#79#92#diseaseC0006142 associated_with peroxiredoxin 6,breast cancer
41#56#gene958879#92#diseaseC0678222 17980029-0#41#56#gene9588 17980029-0#79#92#diseaseC0678222 associated_with peroxiredoxin 6,breast cancer
13#28#gene958890#110#diseaseC0153676 17980029-14#13#28#gene9588 17980029-14#90#110#diseaseC0153676 associated_with peroxiredoxin 6,pulmonary metastases

DisGeNet-2017-sample

Id Subject Object Predicate Lexical cue
T2792 1110-1125 gene:9588 denotes peroxiredoxin 6
T2793 1610-1623 disease:C0006142 denotes breast cancer
R1 T2792 T2793 associated_with peroxiredoxin 6,breast cancer
R2 T2792 T2793 associated_with peroxiredoxin 6,breast cancer

UBERON-AE

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 79-85 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T2 145-151 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T3 493-499 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T4 1610-1616 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T5 2109-2115 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T6 2239-2245 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T7 2587-2593 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T8 1688-1694 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue

performance-test

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 1688-1694 http://purl.obolibrary.org/obo/UBERON_0000479 denotes tissue
PD-UBERON-AE-B_T2 79-85 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T3 145-151 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T4 493-499 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T5 1610-1616 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T6 2109-2115 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T7 2239-2245 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast
PD-UBERON-AE-B_T8 2587-2593 http://purl.obolibrary.org/obo/UBERON_0000310 denotes breast