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PubMed:1797376 JSONTXT

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Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-88 Sentence denotes Synthesis and duplex stability of oligonucleotides containing adenine-guanine analogues.
TextSentencer_T2 89-320 Sentence denotes The nucleosides N6-methoxy-2'-deoxyadenosine (dZ) and 2-amino-9-(2-deoxy-beta-ribofuranosyl)-6-methoxyaminopurine (dK) have been synthesised and converted into 5'-O-dimethoxytrityl 3'-(2-cyanoethyl N,N-diisopropylphosphoramidites).
TextSentencer_T3 321-458 Sentence denotes These monomers have been used in machine DNA synthesis to give a set of heptadecanucleotides containing up to three analogue nucleotides.
TextSentencer_T4 459-629 Sentence denotes The melting transitions (Tm) show that the 17-mer duplexes containing Z.T and Z.C base-pairs have closely similar stabilities, as have those containing K.T and K.C pairs.
TextSentencer_T5 630-759 Sentence denotes They are less stable than the corresponding fully complementary duplexes, but more stable than those containing mismatched pairs.
TextSentencer_T6 760-905 Sentence denotes This, in the case of dZ, is in accord with the amino-imino tautomeric ratio of approximately 1:4 observed for the nucleoside in methyl sulfoxide.
TextSentencer_T7 906-1021 Sentence denotes The application of oligomers containing such "degenerate" bases in oligonucleotide probes and primers is discussed.
T1 0-88 Sentence denotes Synthesis and duplex stability of oligonucleotides containing adenine-guanine analogues.
T2 89-320 Sentence denotes The nucleosides N6-methoxy-2'-deoxyadenosine (dZ) and 2-amino-9-(2-deoxy-beta-ribofuranosyl)-6-methoxyaminopurine (dK) have been synthesised and converted into 5'-O-dimethoxytrityl 3'-(2-cyanoethyl N,N-diisopropylphosphoramidites).
T3 321-458 Sentence denotes These monomers have been used in machine DNA synthesis to give a set of heptadecanucleotides containing up to three analogue nucleotides.
T4 459-629 Sentence denotes The melting transitions (Tm) show that the 17-mer duplexes containing Z.T and Z.C base-pairs have closely similar stabilities, as have those containing K.T and K.C pairs.
T5 630-759 Sentence denotes They are less stable than the corresponding fully complementary duplexes, but more stable than those containing mismatched pairs.
T6 760-905 Sentence denotes This, in the case of dZ, is in accord with the amino-imino tautomeric ratio of approximately 1:4 observed for the nucleoside in methyl sulfoxide.
T7 906-1021 Sentence denotes The application of oligomers containing such "degenerate" bases in oligonucleotide probes and primers is discussed.