PubMed:1794038 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1794038","sourcedb":"PubMed","sourceid":"1794038","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1794038","text":"Purification of the oligosaccharide-cleaving enzymes of Flavobacterium meningosepticum.\nFour oligosaccharide chain-cleaving enzymes, including two new endoglycosidases distinct from endo-beta-acetylglucosaminidase (Endo) F1, have been identified and purified to homogeneity from cultural filtrates of Flavobacterium meningosepticum. FPLC-directed hydrophobic-interaction chromatography in conjunction with high-resolution ion-exchange chromatography provided a more simple, rapid method for the isolation of endoglycosidase F1, F2 and F3, and the amidase, peptide-N4-N-acetyl-beta-D-glucosaminyl)-asparagine amidase (PNGase F), in greater than 50% yield. The specificity of PNGase F and Endo F1 are well established. Endo F2 and Endo F3 represent new distinct endoglycosidases that prefer complex as compared to high-mannose asparagine-linked glycans. 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