PubMed:17823124 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/17823124","sourcedb":"PubMed","sourceid":"17823124","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/17823124","text":"Lipopolysaccharide-mediated interferon regulatory factor activation involves TBK1-IKKepsilon-dependent Lys(63)-linked polyubiquitination and phosphorylation of TANK/I-TRAF.\nType I interferon gene induction relies on IKK-related kinase TBK1 and IKKepsilon-mediated phosphorylations of IRF3/7 through the Toll-like receptor-dependent signaling pathways. The scaffold proteins that assemble these kinase complexes are poorly characterized. We show here that TANK/ITRAF is required for the TBK1- and IKKepsilon-mediated IRF3/7 phosphorylations through some Toll-like receptor-dependent pathways and is part of a TRAF3-containing complex. Moreover, TANK is dispensable for the early phase of double-stranded RNA-mediated IRF3 phosphorylation. Interestingly, TANK is heavily phosphorylated by TBK1-IKKepsilon upon lipopolysaccharide stimulation and is also subject to lipopolysaccharide- and TBK1-IKKepsilon-mediated Lys(63)-linked polyubiquitination, a mechanism that does not require TBK1-IKKepsilon kinase activity. Thus, we have identified TANK as a scaffold protein that assembles some but not all IRF3/7-phosphorylating TBK1-IKKepsilon complexes and demonstrated that these kinases possess two functions, namely the phosphorylation of both IRF3/7 and TANK as well as the recruitment of an E3 ligase for Lys(63)-linked polyubiquitination of their scaffold protein, TANK.","tracks":[{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":172},"obj":"Sentence"},{"id":"T2","span":{"begin":173,"end":351},"obj":"Sentence"},{"id":"T3","span":{"begin":352,"end":436},"obj":"Sentence"},{"id":"T4","span":{"begin":437,"end":633},"obj":"Sentence"},{"id":"T5","span":{"begin":634,"end":737},"obj":"Sentence"},{"id":"T6","span":{"begin":738,"end":1012},"obj":"Sentence"},{"id":"T7","span":{"begin":1013,"end":1369},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"}]},{"project":"bionlp-st-epi-2011-training","denotations":[{"id":"T1","span":{"begin":77,"end":81},"obj":"Protein"},{"id":"T2","span":{"begin":82,"end":92},"obj":"Protein"},{"id":"T3","span":{"begin":160,"end":164},"obj":"Protein"},{"id":"T4","span":{"begin":165,"end":171},"obj":"Protein"},{"id":"T5","span":{"begin":235,"end":239},"obj":"Protein"},{"id":"T6","span":{"begin":244,"end":254},"obj":"Protein"},{"id":"T7","span":{"begin":284,"end":288},"obj":"Protein"},{"id":"T8","span":{"begin":289,"end":290},"obj":"Protein"},{"id":"T9","span":{"begin":455,"end":459},"obj":"Protein"},{"id":"T10","span":{"begin":460,"end":465},"obj":"Protein"},{"id":"T11","span":{"begin":486,"end":490},"obj":"Protein"},{"id":"T12","span":{"begin":496,"end":506},"obj":"Protein"},{"id":"T13","span":{"begin":516,"end":520},"obj":"Protein"},{"id":"T14","span":{"begin":521,"end":522},"obj":"Protein"},{"id":"T15","span":{"begin":608,"end":613},"obj":"Protein"},{"id":"T16","span":{"begin":644,"end":648},"obj":"Protein"},{"id":"T17","span":{"begin":716,"end":720},"obj":"Protein"},{"id":"T18","span":{"begin":753,"end":757},"obj":"Protein"},{"id":"T19","span":{"begin":787,"end":791},"obj":"Protein"},{"id":"T20","span":{"begin":792,"end":802},"obj":"Protein"},{"id":"T21","span":{"begin":886,"end":890},"obj":"Protein"},{"id":"T22","span":{"begin":891,"end":901},"obj":"Protein"},{"id":"T23","span":{"begin":980,"end":984},"obj":"Protein"},{"id":"T24","span":{"begin":985,"end":995},"obj":"Protein"},{"id":"T25","span":{"begin":1038,"end":1042},"obj":"Protein"},{"id":"T26","span":{"begin":1097,"end":1101},"obj":"Protein"},{"id":"T27","span":{"begin":1102,"end":1103},"obj":"Protein"},{"id":"T28","span":{"begin":1120,"end":1124},"obj":"Protein"},{"id":"T29","span":{"begin":1125,"end":1135},"obj":"Protein"},{"id":"T30","span":{"begin":1240,"end":1244},"obj":"Protein"},{"id":"T31","span":{"begin":1245,"end":1246},"obj":"Protein"},{"id":"T32","span":{"begin":1251,"end":1255},"obj":"Protein"},{"id":"T33","span":{"begin":1364,"end":1368},"obj":"Protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T2","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T3","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T4","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T5","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T6","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T7","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T8","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T9","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T10","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T11","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T12","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T13","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T14","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T15","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T16","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T17","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T18","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T19","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T20","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T21","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T22","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T23","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T24","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T25","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T26","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T27","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T28","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T29","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T30","pred":"source","obj":"bionlp-st-epi-2011-training"},{"subj":"T31","pred":"source"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