PubMed:1773433 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1773433","sourcedb":"PubMed","sourceid":"1773433","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1773433","text":"Structural characterization of a new anticoagulant fucan sulfate from the brown seaweed Ecklonia kurome.\nMethylation analysis of a fucose-containing, sulfated polysaccharide (C-II), which was isolated from the brown seaweed Ecklonia kurome and has a potent anticoagulant activity, showed the presence of 3-O- and 3,4-O-disubstituted fucopyranosyl residues in addition to small proportions of nonreducing, terminal fucofuranosyl and fucopyranosyl groups, and 2,3-di-O- and 2,3,4-tri-O-substituted fucopyranosyl and galactopyranosyl residues with various glycosidic linkages. Methanolysis of C-II gave several neutral oligosaccharide fractions in small proportions and two high-molecular-weight acidic fractions in large proportions. Methylation analysis of the low-sulfated acidic fraction showed that the proportion of 3-O-linked fucosyl residues increases and that of 3,4-O-disubstituted decreased as compared to C-II. Methylation and g.l.c.-m.s. analysis of the neutral oligosaccharide fractions showed the presence of Fuc-(1----3)-Fuc and a fucosyl trisaccharide, in addition to small proportions of Gal-(1----4)-Fuc, Fuc-(1----2)-Fuc, Fuc-(1----4)-Fuc, Fuc-(1----2)-Gal, and Fuc----Gal----Fuc. Methylated C-II was also desulfated by methanolysis, followed by remethylation with (2H3)methyl iodide, and most of (2H3)methyl groups were linked to O-4 of the 3-O-linked fucosyl residues. These results suggested a highly branched, new type of fucan sulfate containing a backbone of (1----3)-linked L-fucosyl residues having sulfate groups mainly attached to C-4.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":104},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":105,"end":573},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":574,"end":731},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":732,"end":919},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":920,"end":1197},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":1198,"end":1387},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1388,"end":1562},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":104},"obj":"Sentence"},{"id":"T2","span":{"begin":105,"end":573},"obj":"Sentence"},{"id":"T3","span":{"begin":574,"end":731},"obj":"Sentence"},{"id":"T4","span":{"begin":732,"end":919},"obj":"Sentence"},{"id":"T5","span":{"begin":920,"end":1197},"obj":"Sentence"},{"id":"T6","span":{"begin":1198,"end":1387},"obj":"Sentence"},{"id":"T7","span":{"begin":1388,"end":1562},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":88,"end":103},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":224,"end":239},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"2748578"},{"id":"A2","pred":"db_id","subj":"T2","obj":"2748578"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"GlyCosmos6-Glycan-Motif-Structure","denotations":[{"id":"T1","span":{"begin":131,"end":137},"obj":"https://glytoucan.org/Structures/Glycans/G82576YO"}],"attributes":[{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Structure"}]},{"project":"GlyCosmos6-Glycan-Motif-Image","denotations":[{"id":"T1","span":{"begin":131,"end":137},"obj":"Glycan_Motif"}],"attributes":[{"id":"A1","pred":"image","subj":"T1","obj":"https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G82576YO"},{"subj":"T1","pred":"source","obj":"GlyCosmos6-Glycan-Motif-Image"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":1470,"end":1478},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/UBERON_0001130"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#ccec93","default":true},{"id":"NCBITAXON","color":"#ec93e6"},{"id":"GlyCosmos6-Glycan-Motif-Structure","color":"#93ecd8"},{"id":"GlyCosmos6-Glycan-Motif-Image","color":"#ecbe93"},{"id":"Anatomy-UBERON","color":"#a493ec"}]}]}}