PubMed:1773425 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1773425","sourcedb":"PubMed","sourceid":"1773425","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1773425","text":"Glucuronoxylomannan of Cryptococcus neoformans serotype B: structural analysis by gas-liquid chromatography-mass spectrometry and 13C-nuclear magnetic resonance spectroscopy.\nThe major extracellular polysaccharide (glucuronoxylomannan, GXM) from six strains of Cryptococcus neoformans serotype B was characterized by gas-liquid chromatography (g.l.c.), g.l.c.-mass spectrometry (g.l.c.-m.s.), and nuclear magnetic resonance (n.m.r.) spectroscopy. Ultrasonic irradiation (u.i.) was used to reduce the mol.wt. of native GXM from 9.75 x 10(5) to 1.15 x 10(5) without apparent change in its composition (GXM-S). The Xylp:Manp:GlcpA molar ratio of the GXM and GXM-S from the six strains of C. neoformans serotype B is approximately 3.5:3.0:0.6. GXM-S was O-deacetylated (GXM-D) by treatment with NH4OH. The 13C-n.m.r. analysis of GXM-D gave spectra that served as characteristic fingerprints of the structure and also facilitated the assignment of the anomeric carbon resonances to specific structural moieties present in GXM-D. The GXM-D from each serotype B strain was found to be similar by 13C-n.m.r. spectroscopy. The structure contains a linear (1----3)-alpha-D-Manp backbone substituted with 2-O-beta-GlcpA and 2-O-beta-Xylp. beta-Xylp is also O-4 linked to the Manp substituted with GlcpA. In addition, a model for the disposition of the Xylp and GlcpA side chain substituents along the mannopyranan backbone is proposed, based upon results from the combination of g.l.c.-m.s. and 13C-n.m.r. spectroscopy.","tracks":[{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":174},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":175,"end":446},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":447,"end":607},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":608,"end":739},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":740,"end":797},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":798,"end":1023},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1024,"end":1113},"obj":"Sentence"},{"id":"TextSentencer_T8","span":{"begin":1114,"end":1292},"obj":"Sentence"},{"id":"TextSentencer_T9","span":{"begin":1293,"end":1508},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":174},"obj":"Sentence"},{"id":"T2","span":{"begin":175,"end":446},"obj":"Sentence"},{"id":"T3","span":{"begin":447,"end":607},"obj":"Sentence"},{"id":"T4","span":{"begin":608,"end":739},"obj":"Sentence"},{"id":"T5","span":{"begin":740,"end":797},"obj":"Sentence"},{"id":"T6","span":{"begin":798,"end":1023},"obj":"Sentence"},{"id":"T7","span":{"begin":1024,"end":1113},"obj":"Sentence"},{"id":"T8","span":{"begin":1114,"end":1292},"obj":"Sentence"},{"id":"T9","span":{"begin":1293,"end":1508},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"TextSentencer_T1","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T2","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T3","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T4","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T5","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T6","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T7","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T8","pred":"source","obj":"sentences"},{"subj":"TextSentencer_T9","pred":"source","obj":"sentences"},{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":23,"end":46},"obj":"OrganismTaxon"},{"id":"T2","span":{"begin":261,"end":284},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"5207"},{"id":"A2","pred":"db_id","subj":"T2","obj":"5207"},{"subj":"T1","pred":"source","obj":"NCBITAXON"},{"subj":"T2","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":185,"end":198},"obj":"Body_part"},{"id":"T2","span":{"begin":628,"end":633},"obj":"Body_part"},{"id":"T3","span":{"begin":874,"end":886},"obj":"Body_part"},{"id":"T4","span":{"begin":1168,"end":1176},"obj":"Body_part"},{"id":"T5","span":{"begin":1403,"end":1411},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005576"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/UBERON_0003655"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/UBERON_0013487"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/UBERON_0001130"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0001130"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T2","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T3","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T4","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T5","pred":"source","obj":"Anatomy-UBERON"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"sentences","color":"#93dfec","default":true},{"id":"NCBITAXON","color":"#ecde93"},{"id":"Anatomy-UBERON","color":"#c493ec"}]}]}}