PubMed:17630122 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/17630122","sourcedb":"PubMed","sourceid":"17630122","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/17630122","text":"Preparation and characterization of multilayered hydroxyapatite/silk fibroin film.\nWe prepared multilayered films consisting of silk fibroin (SF) and hydroxyapatite (HAp) by alternating lamination using untreated SF and HAp-deposited SF films. Untreated SF films were prepared from a regenerated SF solution by air drying. HAp-deposited SF films were prepared by soaking methanol-treated SF films containing \u003e5 wt% CaCl2 in a simulated body fluid with the ion concentration 1.5-fold higher than that of the standard one. The multilayered HAp/SF films had HAp layers with approximate thicknesses of 3-5 microm and SF layers with thicknesses of 40-70 microm. The bonding strength between the SF and HAp layers was significantly affected by temperature and compression time under the lamination method. The optimal conditions for achieving the maximum T-peel strength and beta-sheet contents were determined to be 130 degrees C for 4 min. The Young's modulus of the multilayered films (133.4 MPa) was higher than that of the films consisting of SF alone (92.5 MPa) under swollen conditions. The biocompatibility of the HAp-deposited SF films was analyzed by culturing of osteoblasts (MC3T3-E1) on a film. The results indicate that HAp-deposited SF films and SF films show similar degrees of cell adhesion and alkaline phosphatase activities.","tracks":[{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":1168,"end":1179},"obj":"HP_0011846"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]},{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":49,"end":63},"obj":"Chemical:MESH:D017886"},{"id":"T2","span":{"begin":166,"end":169},"obj":"Gene:573"},{"id":"T3","span":{"begin":220,"end":223},"obj":"Gene:573"},{"id":"T4","span":{"begin":323,"end":326},"obj":"Gene:573"},{"id":"T5","span":{"begin":371,"end":379},"obj":"Chemical:MESH:D000432"},{"id":"T6","span":{"begin":415,"end":420},"obj":"Chemical:MESH:D002122"},{"id":"T7","span":{"begin":538,"end":541},"obj":"Gene:573"},{"id":"T8","span":{"begin":555,"end":558},"obj":"Gene:573"},{"id":"T9","span":{"begin":697,"end":700},"obj":"Gene:573"},{"id":"T10","span":{"begin":1116,"end":1119},"obj":"Gene:573"},{"id":"T11","span":{"begin":1228,"end":1231},"obj":"Gene:573"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"},{"subj":"T9","pred":"source","obj":"silkworm"},{"subj":"T10","pred":"source","obj":"silkworm"},{"subj":"T11","pred":"source","obj":"silkworm"}]},{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":49,"end":63},"obj":"Chemical:MESH:D017886"},{"id":"T10","span":{"begin":1116,"end":1119},"obj":"Gene:573"},{"id":"T11","span":{"begin":1228,"end":1231},"obj":"Gene:573"},{"id":"T2","span":{"begin":166,"end":169},"obj":"Gene:573"},{"id":"T3","span":{"begin":220,"end":223},"obj":"Gene:573"},{"id":"T4","span":{"begin":323,"end":326},"obj":"Gene:573"},{"id":"T5","span":{"begin":371,"end":379},"obj":"Chemical:MESH:D000432"},{"id":"T6","span":{"begin":415,"end":420},"obj":"Chemical:MESH:D002122"},{"id":"T7","span":{"begin":538,"end":541},"obj":"Gene:573"},{"id":"T8","span":{"begin":555,"end":558},"obj":"Gene:573"},{"id":"T9","span":{"begin":697,"end":700},"obj":"Gene:573"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkwormbase"},{"subj":"T10","pred":"source","obj":"silkwormbase"},{"subj":"T11","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"},{"subj":"T9","pred":"source","obj":"silkwormbase"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubmedHPO","color":"#93ecb6","default":true},{"id":"silkworm","color":"#ec9c93"},{"id":"silkwormbase","color":"#93a3ec"}]}]}}