| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-88 |
Sentence |
denotes |
Purification and characterization of cellular proteins associated with histone H4 tails. |
| T2 |
89-200 |
Sentence |
denotes |
The histone H4 N-terminal tail has long been regarded as a major regulator in chromatin structure and function. |
| T3 |
201-456 |
Sentence |
denotes |
Although the underlying mechanism has not been unraveled, an emerging body of evidence supports that H4 tail and its post-translational modification function as a recruitment motif for key factors required for proper regulation of chromatin transcription. |
| T4 |
457-637 |
Sentence |
denotes |
To investigate these aspects, we have generated HeLa cell lines that constitutively express ectopic H4 tail domain for biochemical purification of proteins associated with H4 tail. |
| T5 |
638-822 |
Sentence |
denotes |
We found that expressed H4 tails stably associate with sets of transcription regulatory factors and histone methyltransferases distinct from those that associate with histone H3 tails. |
| T6 |
823-1147 |
Sentence |
denotes |
Importantly, point mutations of four major lysine substrates to block cellular acetylation of ectopic H4 tail significantly inhibited the association of histone methyltransferases and sets of transcription-activating factors, supporting a major role of acetylation on recruitmentbased action of H4 tail during transcription. |
| T7 |
1148-1350 |
Sentence |
denotes |
Further, our transcription analysis revealed that the proteins associated with wild-type/acetylated H4 tail, but not with mutant/unacetylated H4 tail, can enhance p300-dependent chromatin transcription. |
| T8 |
1351-1556 |
Sentence |
denotes |
Taken together, these findings demonstrate novel roles for H4 tail and its acetylation in mediating recruitment of multiple regulatory factors that can change chromatin states for transcription regulation. |
