PubMed:1733809 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1733809","sourcedb":"PubMed","sourceid":"1733809","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1733809","text":"Insulin stimulates accumulation and efflux of macromolecules in isolated nuclei from H35 hepatoma cells.\nThis study used 10-nm gold particles with 5-7 insulin molecules attached (Au10-Ins) to investigate the site of interaction of insulin with the nuclear envelope during insulin uptake into intact isolated nuclei. Despite its size, and in the absence of ATP, Au10-Ins entered nuclei through the nuclear pore and associated with the heterochromatin. Because Au10-Ins is essentially gold-bovine serum albumin (Au-BSA) with a few insulin molecules attached, the effect of insulin and other growth factors on the nuclear accumulation of BSA coupled to 10-, 15-, and 24-nm-diam colloidal gold particles (Au10-BSA, Au15-BSA, and Au24-BSA) was determined. The Au-BSA complexes were excluded from nuclei in the absence of insulin. Insulin (0.5-100 ng/ml) caused a dose-dependent accumulation of Au10-BSA in the nucleus. The nuclear membrane was shown to be intact by several criteria, therefore, accumulation of Au-BSA occurred via the nuclear pore and was not due to leakage across or through the membrane. Uptake of 15- and 24-nm Au-BSA molecules was not affected by insulin, suggesting the hormone had a limited effect in increasing the functional diameter of the nuclear pores. Glucagon, epidermal growth factor, platelet-derived growth factor, insulinlike growth factor I, and insulin A or B chains did not stimulate the accumulation of Au10-BSA. The insulin-stimulated accumulation of Au10-BSA was blocked by concanavalin A, mimicked by wheat-germ agglutinin, and did not require ATP. The Au10-BSA in the nucleus was associated with heterochromatin, suggesting it bound to a nuclear element.(ABSTRACT TRUNCATED AT 250 WORDS)","tracks":[{"project":"LitCoin-PubTator-for-Tuning","denotations":[{"id":"1","span":{"begin":89,"end":97},"obj":"DiseaseOrPhenotypicFeature"},{"id":"10","span":{"begin":356,"end":359},"obj":"ChemicalEntity"},{"id":"11","span":{"begin":488,"end":494},"obj":"OrganismTaxon"},{"id":"12","span":{"begin":495,"end":508},"obj":"GeneOrGeneProduct"},{"id":"13","span":{"begin":755,"end":757},"obj":"ChemicalEntity"},{"id":"14","span":{"begin":1126,"end":1128},"obj":"ChemicalEntity"},{"id":"15","span":{"begin":1343,"end":1361},"obj":"DiseaseOrPhenotypicFeature"},{"id":"16","span":{"begin":1537,"end":1542},"obj":"OrganismTaxon"},{"id":"17","span":{"begin":1580,"end":1583},"obj":"ChemicalEntity"}],"attributes":[{"id":"A14","pred":"tao:has_database_id","subj":"14","obj":"MESH:D006046"},{"id":"A16","pred":"tao:has_database_id","subj":"16","obj":"Tax:4565"},{"id":"A15","pred":"tao:has_database_id","subj":"15","obj":"MESH:D006130"},{"id":"A12","pred":"tao:has_database_id","subj":"12","obj":"Gene:24186"},{"id":"A1","pred":"tao:has_database_id","subj":"1","obj":"MESH:D006528"},{"id":"A10","pred":"tao:has_database_id","subj":"10","obj":"MESH:D000255"},{"id":"A13","pred":"tao:has_database_id","subj":"13","obj":"MESH:D006046"},{"id":"A17","pred":"tao:has_database_id","subj":"17","obj":"MESH:D000255"},{"id":"A11","pred":"tao:has_database_id","subj":"11","obj":"Tax:9913"},{"subj":"1","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"10","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"11","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"12","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"13","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"14","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"15","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"16","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"17","pred":"source","obj":"LitCoin-PubTator-for-Tuning"}]},{"project":"LitCoin-Disease-Tuning-1","denotations":[{"id":"T1","span":{"begin":89,"end":97},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"ID:","subj":"T1","obj":"D006528"},{"subj":"T1","pred":"source","obj":"LitCoin-Disease-Tuning-1"}]},{"project":"LitEisuke","denotations":[{"id":"T1","span":{"begin":89,"end":97},"obj":"DiseaseOrPhenotypicFeature"}],"attributes":[{"id":"A1","pred":"#label","subj":"T1","obj":"D006528"},{"subj":"T1","pred":"source","obj":"LitEisuke"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"LitCoin-PubTator-for-Tuning","color":"#ec93be","default":true},{"id":"LitCoin-Disease-Tuning-1","color":"#93d8ec"},{"id":"LitEisuke","color":"#ece693"}]}]}}