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PubMed:1730728 JSONTXT

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CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 1237-1248 Cell denotes fibroblasts http://purl.obolibrary.org/obo/CL:0000057

GlyCosmos6-Glycan-Motif-Image

Id Subject Object Predicate Lexical cue image
T1 1137-1144 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO
T3 1698-1705 Glycan_Motif denotes heparin https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G54161DR|https://api.glycosmos.org/wurcs2image/0.10.0/png/binary/G00021MO

sentences

Id Subject Object Predicate Lexical cue
T1 0-179 Sentence denotes The functional characteristics of a human apolipoprotein E variant (cysteine at residue 142) may explain its association with dominant expression of type III hyperlipoproteinemia.
T2 180-295 Sentence denotes Type III hyperlipoproteinemia typically is associated with homozygosity for apolipoprotein (apo) E2(Arg158----Cys).
T3 296-475 Sentence denotes Dominant expression of type III hyperlipoproteinemia associated with apoE phenotype E3/3 is caused by heterozygosity for a human apoE variant, apoE3(Cys112----Arg, Arg142----Cys).
T4 476-663 Sentence denotes However, this apoE3 variant was not separable from the normal apoE3 in these patients' plasma because the two proteins have identical amino acid composition, charge, and molecular weight.
T5 664-812 Sentence denotes Therefore, to determine the functional characteristics of this protein, we used recombinant DNA techniques to produce this apoE variant in bacteria.
T6 813-940 Sentence denotes We also produced a non-naturally occurring variant, apoE(Arg142----Cys), that had only the cysteine substituted at residue 142.
T7 941-1170 Sentence denotes These two apoE variants were purified from cell lysates of the transfected Escherichia coli by ultracentrifugal flotation in the presence of phospholipid, by gel filtration chromatography, and by heparin-Sepharose chromatography.
T8 1171-1296 Sentence denotes Both Cys142 apoE variants bound to lipoprotein receptors on human fibroblasts with only about 20% of normal binding activity.
T9 1297-1437 Sentence denotes Therefore, cysteine at residue 142, not arginine at residue 112, is responsible for the decreased receptor binding activity of the variants.
T10 1438-1622 Sentence denotes Cysteamine treatment and removal of the carboxyl-terminal domain had little effect on the binding activity, whereas both modulate the receptor binding activity of apoE2(Arg158----Cys).
T11 1623-1922 Sentence denotes The mutation at residue 142 decreased the binding activity of apoE to both heparin and the monoclonal antibody 1D7 (this antibody inhibits receptor binding of apoE), whereas apoE2(Arg158----Cys), which is associated with recessive expression of type III hyperlipoproteinemia, binds normally to both.
T12 1923-2120 Sentence denotes The Arg112, Cys142 variant predominantes 3:1 over normal apoE3 in the very low density lipoproteins of plasma from an affected subject, as assessed by differential reactivity with the antibody 1D7.
T13 2121-2309 Sentence denotes The unique combination of functional properties of the Arg112, Cys142 variant provides a possible explanation for its association with dominant expression of type III hyperlipoproteinemia.
T1 0-179 Sentence denotes The functional characteristics of a human apolipoprotein E variant (cysteine at residue 142) may explain its association with dominant expression of type III hyperlipoproteinemia.
T2 180-295 Sentence denotes Type III hyperlipoproteinemia typically is associated with homozygosity for apolipoprotein (apo) E2(Arg158----Cys).
T3 296-475 Sentence denotes Dominant expression of type III hyperlipoproteinemia associated with apoE phenotype E3/3 is caused by heterozygosity for a human apoE variant, apoE3(Cys112----Arg, Arg142----Cys).
T4 476-663 Sentence denotes However, this apoE3 variant was not separable from the normal apoE3 in these patients' plasma because the two proteins have identical amino acid composition, charge, and molecular weight.
T5 664-812 Sentence denotes Therefore, to determine the functional characteristics of this protein, we used recombinant DNA techniques to produce this apoE variant in bacteria.
T6 813-940 Sentence denotes We also produced a non-naturally occurring variant, apoE(Arg142----Cys), that had only the cysteine substituted at residue 142.
T7 941-1170 Sentence denotes These two apoE variants were purified from cell lysates of the transfected Escherichia coli by ultracentrifugal flotation in the presence of phospholipid, by gel filtration chromatography, and by heparin-Sepharose chromatography.
T8 1171-1296 Sentence denotes Both Cys142 apoE variants bound to lipoprotein receptors on human fibroblasts with only about 20% of normal binding activity.
T9 1297-1437 Sentence denotes Therefore, cysteine at residue 142, not arginine at residue 112, is responsible for the decreased receptor binding activity of the variants.
T10 1438-1622 Sentence denotes Cysteamine treatment and removal of the carboxyl-terminal domain had little effect on the binding activity, whereas both modulate the receptor binding activity of apoE2(Arg158----Cys).
T11 1623-1922 Sentence denotes The mutation at residue 142 decreased the binding activity of apoE to both heparin and the monoclonal antibody 1D7 (this antibody inhibits receptor binding of apoE), whereas apoE2(Arg158----Cys), which is associated with recessive expression of type III hyperlipoproteinemia, binds normally to both.
T12 1923-2120 Sentence denotes The Arg112, Cys142 variant predominantes 3:1 over normal apoE3 in the very low density lipoproteins of plasma from an affected subject, as assessed by differential reactivity with the antibody 1D7.
T13 2121-2309 Sentence denotes The unique combination of functional properties of the Arg112, Cys142 variant provides a possible explanation for its association with dominant expression of type III hyperlipoproteinemia.

GlyCosmos6-Glycan-Motif-Structure

Id Subject Object Predicate Lexical cue
T1 1137-1144 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T2 1137-1144 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin
T3 1698-1705 https://glytoucan.org/Structures/Glycans/G00021MO denotes heparin
T4 1698-1705 https://glytoucan.org/Structures/Glycans/G54161DR denotes heparin

DisGeNET

Id Subject Object Predicate Lexical cue
T0 42-58 gene:348 denotes apolipoprotein E
T1 149-178 disease:C0020479 denotes type III hyperlipoproteinemia
R1 T0 T1 associated_with apolipoprotein E,type III hyperlipoproteinemia

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 189-209 HP_0010980 denotes hyperlipoproteinemia
T2 328-348 HP_0010980 denotes hyperlipoproteinemia
T3 1877-1897 HP_0010980 denotes hyperlipoproteinemia
T4 2288-2308 HP_0010980 denotes hyperlipoproteinemia

DisGeNET5_variant_disease

Id Subject Object Predicate Lexical cue
1730728-10#180#193#geners11542041 1803-1816 geners11542041 denotes Arg158----Cys
1730728-10#245#274#diseaseC0020479 1868-1897 diseaseC0020479 denotes type III hyperlipoproteinemia
180#193#geners11542041245#274#diseaseC0020479 1730728-10#180#193#geners11542041 1730728-10#245#274#diseaseC0020479 associated_with Arg158----Cys,type III hyperlipoproteinemia

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
1730728-0#42#58#gene348 42-58 gene348 denotes apolipoprotein E
1730728-0#149#178#diseaseC0020479 149-178 diseaseC0020479 denotes type III hyperlipoproteinemia
42#58#gene348149#178#diseaseC0020479 1730728-0#42#58#gene348 1730728-0#149#178#diseaseC0020479 associated_with apolipoprotein E,type III hyperlipoproteinemia

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 158-178 Phenotype denotes hyperlipoproteinemia HP:0010980
T2 189-209 Phenotype denotes hyperlipoproteinemia HP:0010980
T3 328-348 Phenotype denotes hyperlipoproteinemia HP:0010980
T4 1877-1897 Phenotype denotes hyperlipoproteinemia HP:0010980
T5 2288-2308 Phenotype denotes hyperlipoproteinemia HP:0010980

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 158-178 Disease denotes hyperlipoproteinemia http://purl.obolibrary.org/obo/MONDO_0037748
T2 189-209 Disease denotes hyperlipoproteinemia http://purl.obolibrary.org/obo/MONDO_0037748
T3 328-348 Disease denotes hyperlipoproteinemia http://purl.obolibrary.org/obo/MONDO_0037748
T4 1877-1897 Disease denotes hyperlipoproteinemia http://purl.obolibrary.org/obo/MONDO_0037748
T5 2288-2308 Disease denotes hyperlipoproteinemia http://purl.obolibrary.org/obo/MONDO_0037748

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 36-41 OrganismTaxon denotes human 9606
T2 419-424 OrganismTaxon denotes human 9606
T3 803-811 OrganismTaxon denotes bacteria 2|629395
T5 1016-1032 OrganismTaxon denotes Escherichia coli 562
T6 1231-1236 OrganismTaxon denotes human 9606

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 563-569 Body_part denotes plasma http://purl.obolibrary.org/obo/UBERON_0001969
T2 1237-1248 Body_part denotes fibroblasts http://purl.obolibrary.org/obo/CL_0000057
T3 2026-2032 Body_part denotes plasma http://purl.obolibrary.org/obo/UBERON_0001969