PubMed:17284839 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/17284839","sourcedb":"PubMed","sourceid":"17284839","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/17284839","text":"Expression and characterization of a sigma-class glutathione S-transferase of the fall webworm, Hyphantria cunea.\nA cDNA encoding glutathione S-transferase (GST) of the fall webworm, Hyphantria cunea, was cloned by reverse transcriptase-polymerase chain reaction. The resulting clone (hcGST) was sequenced and deduced for amino acid sequence, which revealed 87, 59, and 42% identities to Sigma-class GSTs from Bombyx mori, Manduca sexta, and Blattella germanica respectively. A recombinant hcGST protein (rhcGST) was functionally overexpressed in Escherichia coli cells in a soluble form and purified to homogeneity. rhcGST retained more than 75% of its original GST activity after incubation at pHs 6 to 11. Incubation for 30 min at temperatures below 50 degrees C scarcely affected the activity. rhcGST was able to catalyze the reaction of glutathione with 1-chloro-2,4-dinitrobenzene, a universal substrate for GST, as well as with 4-hydroxynonenal, a product of lipid peroxidation. We also found that as compared to B. mori Sigma-class GST, rhcGST had a higher affinity for fenitrothion, an organophosphorus insecticide.","tracks":[{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":169,"end":173},"obj":"HP_0002527"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"}]},{"project":"silkworm","denotations":[{"id":"T1","span":{"begin":96,"end":112},"obj":"Species:39466"},{"id":"T2","span":{"begin":183,"end":199},"obj":"Species:39466"},{"id":"T3","span":{"begin":410,"end":421},"obj":"Species:7091"},{"id":"T4","span":{"begin":423,"end":436},"obj":"Species:7130"},{"id":"T5","span":{"begin":442,"end":461},"obj":"Species:6973"},{"id":"T6","span":{"begin":547,"end":563},"obj":"Species:562"},{"id":"T7","span":{"begin":842,"end":853},"obj":"Chemical:MESH:D005978"},{"id":"T8","span":{"begin":859,"end":886},"obj":"Chemical:MESH:D004137"},{"id":"T9","span":{"begin":935,"end":951},"obj":"Chemical:MESH:C027576"},{"id":"T10","span":{"begin":966,"end":971},"obj":"Chemical:MESH:D008055"},{"id":"T11","span":{"begin":1020,"end":1027},"obj":"Species:7091"},{"id":"T12","span":{"begin":1078,"end":1090},"obj":"Chemical:MESH:D005278"},{"id":"T13","span":{"begin":1095,"end":1111},"obj":"Chemical:-"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkworm"},{"subj":"T2","pred":"source","obj":"silkworm"},{"subj":"T3","pred":"source","obj":"silkworm"},{"subj":"T4","pred":"source","obj":"silkworm"},{"subj":"T5","pred":"source","obj":"silkworm"},{"subj":"T6","pred":"source","obj":"silkworm"},{"subj":"T7","pred":"source","obj":"silkworm"},{"subj":"T8","pred":"source","obj":"silkworm"},{"subj":"T9","pred":"source","obj":"silkworm"},{"subj":"T10","pred":"source","obj":"silkworm"},{"subj":"T11","pred":"source","obj":"silkworm"},{"subj":"T12","pred":"source","obj":"silkworm"},{"subj":"T13","pred":"source","obj":"silkworm"}]},{"project":"silkwormbase","denotations":[{"id":"T1","span":{"begin":96,"end":112},"obj":"Species:39466"},{"id":"T10","span":{"begin":966,"end":971},"obj":"Chemical:MESH:D008055"},{"id":"T11","span":{"begin":1020,"end":1027},"obj":"Species:7091"},{"id":"T12","span":{"begin":1078,"end":1090},"obj":"Chemical:MESH:D005278"},{"id":"T13","span":{"begin":1095,"end":1111},"obj":"Chemical:-"},{"id":"T2","span":{"begin":183,"end":199},"obj":"Species:39466"},{"id":"T3","span":{"begin":410,"end":421},"obj":"Species:7091"},{"id":"T4","span":{"begin":423,"end":436},"obj":"Species:7130"},{"id":"T5","span":{"begin":442,"end":461},"obj":"Species:6973"},{"id":"T6","span":{"begin":547,"end":563},"obj":"Species:562"},{"id":"T7","span":{"begin":842,"end":853},"obj":"Chemical:MESH:D005978"},{"id":"T8","span":{"begin":859,"end":886},"obj":"Chemical:MESH:D004137"},{"id":"T9","span":{"begin":935,"end":951},"obj":"Chemical:MESH:C027576"}],"attributes":[{"subj":"T1","pred":"source","obj":"silkwormbase"},{"subj":"T10","pred":"source","obj":"silkwormbase"},{"subj":"T11","pred":"source","obj":"silkwormbase"},{"subj":"T12","pred":"source","obj":"silkwormbase"},{"subj":"T13","pred":"source","obj":"silkwormbase"},{"subj":"T2","pred":"source","obj":"silkwormbase"},{"subj":"T3","pred":"source","obj":"silkwormbase"},{"subj":"T4","pred":"source","obj":"silkwormbase"},{"subj":"T5","pred":"source","obj":"silkwormbase"},{"subj":"T6","pred":"source","obj":"silkwormbase"},{"subj":"T7","pred":"source","obj":"silkwormbase"},{"subj":"T8","pred":"source","obj":"silkwormbase"},{"subj":"T9","pred":"source","obj":"silkwormbase"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"PubmedHPO","color":"#ece093","default":true},{"id":"silkworm","color":"#c693ec"},{"id":"silkwormbase","color":"#93ecac"}]}]}}