PubMed:1722683 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/1722683","sourcedb":"PubMed","sourceid":"1722683","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/1722683","text":"cDNA cloning and expression of a human FGF receptor which binds acidic and basic FGF.\nWe have isolated and characterized a cDNA clone, phFGFR, encoding a human fibroblast growth factor (FGF) receptor. phFGFR contains an open reading frame which encodes an 820 amino acid polypeptide with three immunoglobulin-like domains in the extracellular part and an intracellular split tyrosine kinase domain. Transient expression in COS-1 cells and immunoprecipitation using an antiserum raised against a C-terminal peptide, gave rise to two components, representing mature (130 kDa) and precursor (115 kDa) forms of the phFGFR encoded polypeptide, which was denoted hFGFR-1. Crosslinking of iodinated acidic FGF (aFGF) and basic FGF (bFGF) to transiently expressing COS-1 cells revealed a major band of 95 kDa, which was competed for by both aFGF and bFGF. From Scatchard analyses, the Kd:s for binding of aFGF and bFGF to hFGFR-1 were estimated to 25 pM and 41 pM, respectively. Thus, phFGFR encodes a human FGF receptor with high affinity for both aFGF and bFGF.","tracks":[{"project":"AIMed","denotations":[{"id":"T1","span":{"begin":135,"end":141},"obj":"protein"},{"id":"T2","span":{"begin":201,"end":207},"obj":"protein"},{"id":"T3","span":{"begin":611,"end":617},"obj":"protein"},{"id":"T4","span":{"begin":657,"end":664},"obj":"protein"},{"id":"T5","span":{"begin":692,"end":702},"obj":"protein"},{"id":"T6","span":{"begin":704,"end":708},"obj":"protein"},{"id":"T7","span":{"begin":714,"end":723},"obj":"protein"},{"id":"T8","span":{"begin":725,"end":729},"obj":"protein"},{"id":"T9","span":{"begin":833,"end":837},"obj":"protein"},{"id":"T10","span":{"begin":842,"end":846},"obj":"protein"},{"id":"T11","span":{"begin":897,"end":901},"obj":"protein"},{"id":"T12","span":{"begin":906,"end":910},"obj":"protein"},{"id":"T13","span":{"begin":914,"end":921},"obj":"protein"},{"id":"T14","span":{"begin":977,"end":983},"obj":"protein"},{"id":"T15","span":{"begin":1041,"end":1045},"obj":"protein"},{"id":"T16","span":{"begin":1050,"end":1054},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"AIMed"},{"subj":"T2","pred":"source","obj":"AIMed"},{"subj":"T3","pred":"source","obj":"AIMed"},{"subj":"T4","pred":"source","obj":"AIMed"},{"subj":"T5","pred":"source","obj":"AIMed"},{"subj":"T6","pred":"source","obj":"AIMed"},{"subj":"T7","pred":"source","obj":"AIMed"},{"subj":"T8","pred":"source","obj":"AIMed"},{"subj":"T9","pred":"source","obj":"AIMed"},{"subj":"T10","pred":"source","obj":"AIMed"},{"subj":"T11","pred":"source","obj":"AIMed"},{"subj":"T12","pred":"source","obj":"AIMed"},{"subj":"T13","pred":"source","obj":"AIMed"},{"subj":"T14","pred":"source","obj":"AIMed"},{"subj":"T15","pred":"source","obj":"AIMed"},{"subj":"T16","pred":"source","obj":"AIMed"}]},{"project":"FSU-PRGE","denotations":[{"id":"T1","span":{"begin":39,"end":51},"obj":"protein"},{"id":"T2","span":{"begin":64,"end":84},"obj":"protein"},{"id":"T3","span":{"begin":135,"end":141},"obj":"protein"},{"id":"T4","span":{"begin":160,"end":199},"obj":"protein"},{"id":"T5","span":{"begin":201,"end":207},"obj":"protein"},{"id":"T6","span":{"begin":294,"end":308},"obj":"protein"},{"id":"T7","span":{"begin":375,"end":390},"obj":"protein"},{"id":"T8","span":{"begin":611,"end":617},"obj":"protein"},{"id":"T9","span":{"begin":657,"end":664},"obj":"protein"},{"id":"T10","span":{"begin":692,"end":702},"obj":"protein"},{"id":"T11","span":{"begin":704,"end":708},"obj":"protein"},{"id":"T12","span":{"begin":714,"end":723},"obj":"protein"},{"id":"T13","span":{"begin":725,"end":729},"obj":"protein"},{"id":"T14","span":{"begin":833,"end":837},"obj":"protein"},{"id":"T15","span":{"begin":842,"end":846},"obj":"protein"},{"id":"T16","span":{"begin":897,"end":901},"obj":"protein"},{"id":"T17","span":{"begin":906,"end":910},"obj":"protein"},{"id":"T18","span":{"begin":914,"end":921},"obj":"protein"},{"id":"T19","span":{"begin":977,"end":983},"obj":"protein"},{"id":"T20","span":{"begin":1000,"end":1012},"obj":"protein"},{"id":"T21","span":{"begin":1041,"end":1045},"obj":"protein"},{"id":"T22","span":{"begin":1050,"end":1054},"obj":"protein"}],"attributes":[{"subj":"T1","pred":"source","obj":"FSU-PRGE"},{"subj":"T2","pred":"source","obj":"FSU-PRGE"},{"subj":"T3","pred":"source","obj":"FSU-PRGE"},{"subj":"T4","pred":"source","obj":"FSU-PRGE"},{"subj":"T5","pred":"source","obj":"FSU-PRGE"},{"subj":"T6","pred":"source","obj":"FSU-PRGE"},{"subj":"T7","pred":"source","obj":"FSU-PRGE"},{"subj":"T8","pred":"source","obj":"FSU-PRGE"},{"subj":"T9","pred":"source","obj":"FSU-PRGE"},{"subj":"T10","pred":"source","obj":"FSU-PRGE"},{"subj":"T11","pred":"source","obj":"FSU-PRGE"},{"subj":"T12","pred":"source","obj":"FSU-PRGE"},{"subj":"T13","pred":"source","obj":"FSU-PRGE"},{"subj":"T14","pred":"source","obj":"FSU-PRGE"},{"subj":"T15","pred":"source","obj":"FSU-PRGE"},{"subj":"T16","pred":"source","obj":"FSU-PRGE"},{"subj":"T17","pred":"source","obj":"FSU-PRGE"},{"subj":"T18","pred":"source","obj":"FSU-PRGE"},{"subj":"T19","pred":"source","obj":"FSU-PRGE"},{"subj":"T20","pred":"source","obj":"FSU-PRGE"},{"subj":"T21","pred":"source","obj":"FSU-PRGE"},{"subj":"T22","pred":"source","obj":"FSU-PRGE"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"AIMed","color":"#ec93af","default":true},{"id":"FSU-PRGE","color":"#93c9ec"}]}]}}