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CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 125-152 Cell denotes Intestinal epithelial cells http://purl.obolibrary.org/obo/CL:0002563
T2 1277-1282 Cell denotes delta http://purl.obolibrary.org/obo/CL:0004124

sentences

Id Subject Object Predicate Lexical cue
T1 0-91 Sentence denotes Functional insertion of the SV40 large T oncogene in cystic fibrosis intestinal epithelium.
T2 92-124 Sentence denotes Characterization of CFI-3 cells.
T3 125-284 Sentence denotes Intestinal epithelial cells were isolated from a fetus with cystic fibrosis (CF) and transfected with a plasmid vector recombined with the ori- mutant of SV40.
T4 285-364 Sentence denotes A population of proliferative cells was then subcloned and designated as CFI-3.
T5 365-458 Sentence denotes These cells had a doubling time of 24 h and were maintained in culture for up to 25 passages.
T6 459-525 Sentence denotes At passage 8, CFI-3 cells did not produce any tumors in nude mice.
T7 526-663 Sentence denotes Northern blot and immunofluorescence studies indicated that the extended lifespan of CFI-3 cells results in genomic insertion of SV40 LT.
T8 664-832 Sentence denotes Intestinal CFI-3 cells are epithelial, according to the expression of the human cytokeratin 18 gene and poorly differentiated by phase-contrast and electron microscopy.
T9 833-1040 Sentence denotes Functional membrane receptors activated by vasoactive intestinal peptide (VIP), its natural analogue pituitary adenylate cyclase activating peptide (PACAP-38), and isoproterenol were observed in CFI-3 cells.
T10 1041-1198 Sentence denotes Restriction fragment length polymorphism analysis of the PstI KM19 site revealed that the cftr locus was identical in the chorionic villi and in CFI-3 cells.
T11 1199-1362 Sentence denotes The manifestation of CF in this family was not related to the common mutation delta F508, since this fetus was heterozygous for the substitutions S549N and N1303K.
T12 1363-1622 Sentence denotes Chloride transport, assessed by the 125I efflux, was induced in CFI-3 cells by the calcium inophore ionomycin, but not by the adenylate cyclase activator forskolin, and was inhibited by the chloride channel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid.
T13 1623-1810 Sentence denotes These results were confirmed in patch clamp studies in which the cpt cAMP analogue failed to stimulate membrane currents, while the calcium ionophore ionomycin stimulated inward currents.
T14 1811-2124 Sentence denotes We conclude that intestinal CFI-3 cells retain the CF phenotype relating to defective regulation of Cl- channels, and therefore constitute a suitable model, 1) for elucidating the function of CFTR protein, 2) developing new therapeutic agents, and 3) correcting the CF defect by gene replacement therapy in vitro.
T1 0-91 Sentence denotes Functional insertion of the SV40 large T oncogene in cystic fibrosis intestinal epithelium.
T2 92-124 Sentence denotes Characterization of CFI-3 cells.
T3 125-284 Sentence denotes Intestinal epithelial cells were isolated from a fetus with cystic fibrosis (CF) and transfected with a plasmid vector recombined with the ori- mutant of SV40.
T4 285-364 Sentence denotes A population of proliferative cells was then subcloned and designated as CFI-3.
T5 365-458 Sentence denotes These cells had a doubling time of 24 h and were maintained in culture for up to 25 passages.
T6 459-525 Sentence denotes At passage 8, CFI-3 cells did not produce any tumors in nude mice.
T7 526-663 Sentence denotes Northern blot and immunofluorescence studies indicated that the extended lifespan of CFI-3 cells results in genomic insertion of SV40 LT.
T8 664-832 Sentence denotes Intestinal CFI-3 cells are epithelial, according to the expression of the human cytokeratin 18 gene and poorly differentiated by phase-contrast and electron microscopy.
T9 833-1040 Sentence denotes Functional membrane receptors activated by vasoactive intestinal peptide (VIP), its natural analogue pituitary adenylate cyclase activating peptide (PACAP-38), and isoproterenol were observed in CFI-3 cells.
T10 1041-1198 Sentence denotes Restriction fragment length polymorphism analysis of the PstI KM19 site revealed that the cftr locus was identical in the chorionic villi and in CFI-3 cells.
T11 1199-1362 Sentence denotes The manifestation of CF in this family was not related to the common mutation delta F508, since this fetus was heterozygous for the substitutions S549N and N1303K.
T12 1363-1622 Sentence denotes Chloride transport, assessed by the 125I efflux, was induced in CFI-3 cells by the calcium inophore ionomycin, but not by the adenylate cyclase activator forskolin, and was inhibited by the chloride channel blocker 5-nitro-2-(3-phenylpropylamino)benzoic acid.
T13 1623-1810 Sentence denotes These results were confirmed in patch clamp studies in which the cpt cAMP analogue failed to stimulate membrane currents, while the calcium ionophore ionomycin stimulated inward currents.
T14 1811-2124 Sentence denotes We conclude that intestinal CFI-3 cells retain the CF phenotype relating to defective regulation of Cl- channels, and therefore constitute a suitable model, 1) for elucidating the function of CFTR protein, 2) developing new therapeutic agents, and 3) correcting the CF defect by gene replacement therapy in vitro.

Glycosmos6-MAT

Id Subject Object Predicate Lexical cue
T1 69-79 http://purl.obolibrary.org/obo/MAT_0000043 denotes intestinal
T2 125-135 http://purl.obolibrary.org/obo/MAT_0000043 denotes Intestinal
T3 174-179 http://purl.obolibrary.org/obo/MAT_0000399 denotes fetus
T4 664-674 http://purl.obolibrary.org/obo/MAT_0000043 denotes Intestinal
T5 887-897 http://purl.obolibrary.org/obo/MAT_0000043 denotes intestinal
T6 934-943 http://purl.obolibrary.org/obo/MAT_0000077 denotes pituitary
T7 1300-1305 http://purl.obolibrary.org/obo/MAT_0000399 denotes fetus
T8 1828-1838 http://purl.obolibrary.org/obo/MAT_0000043 denotes intestinal

DisGeNET

Id Subject Object Predicate Lexical cue
T0 112-115 gene:3426 denotes CFI
T1 53-68 disease:C0010674 denotes cystic fibrosis
R1 T0 T1 associated_with CFI,cystic fibrosis

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 505-511 HP_0002664 denotes tumors
T1 505-511 HP_0002664 denotes tumors

DisGeNET5_variant_disease

Id Subject Object Predicate Lexical cue
1718974-8#156#162#geners80034486 1355-1361 geners80034486 denotes N1303K
1718974-8#146#151#geners121908755 1345-1350 geners121908755 denotes S549N
1718974-8#146#151#geners747607158 1345-1350 geners747607158 denotes S549N
1718974-8#21#23#diseaseC0010674 1220-1222 diseaseC0010674 denotes CF
156#162#geners8003448621#23#diseaseC0010674 1718974-8#156#162#geners80034486 1718974-8#21#23#diseaseC0010674 associated_with N1303K,CF
146#151#geners12190875521#23#diseaseC0010674 1718974-8#146#151#geners121908755 1718974-8#21#23#diseaseC0010674 associated_with S549N,CF
146#151#geners74760715821#23#diseaseC0010674 1718974-8#146#151#geners747607158 1718974-8#21#23#diseaseC0010674 associated_with S549N,CF

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
1718974-0#112#115#gene3426 112-115 gene3426 denotes CFI
1718974-0#53#68#diseaseC0010674 53-68 diseaseC0010674 denotes cystic fibrosis
1718974-11#192#196#gene1080 2003-2007 gene1080 denotes CFTR
1718974-11#51#53#diseaseC0010674 1862-1864 diseaseC0010674 denotes CF
1718974-11#266#268#diseaseC0010674 2077-2079 diseaseC0010674 denotes CF
1718974-6#43#72#gene7432 876-905 gene7432 denotes vasoactive intestinal peptide
1718974-6#74#77#gene7432 907-910 gene7432 denotes VIP
1718974-6#195#198#gene3426 1028-1031 gene3426 denotes CFI
1718974-6#101#110#diseaseC0032002 934-943 diseaseC0032002 denotes pituitary
112#115#gene342653#68#diseaseC0010674 1718974-0#112#115#gene3426 1718974-0#53#68#diseaseC0010674 associated_with CFI,cystic fibrosis
192#196#gene108051#53#diseaseC0010674 1718974-11#192#196#gene1080 1718974-11#51#53#diseaseC0010674 associated_with CFTR,CF
192#196#gene1080266#268#diseaseC0010674 1718974-11#192#196#gene1080 1718974-11#266#268#diseaseC0010674 associated_with CFTR,CF
43#72#gene7432101#110#diseaseC0032002 1718974-6#43#72#gene7432 1718974-6#101#110#diseaseC0032002 associated_with vasoactive intestinal peptide,pituitary
74#77#gene7432101#110#diseaseC0032002 1718974-6#74#77#gene7432 1718974-6#101#110#diseaseC0032002 associated_with VIP,pituitary
195#198#gene3426101#110#diseaseC0032002 1718974-6#195#198#gene3426 1718974-6#101#110#diseaseC0032002 associated_with CFI,pituitary

mondo_disease

Id Subject Object Predicate Lexical cue mondo_id
T1 53-68 Disease denotes cystic fibrosis http://purl.obolibrary.org/obo/MONDO_0009061
T2 185-200 Disease denotes cystic fibrosis http://purl.obolibrary.org/obo/MONDO_0009061
T3 202-204 Disease denotes CF http://purl.obolibrary.org/obo/MONDO_0009061
T4 505-511 Disease denotes tumors http://purl.obolibrary.org/obo/MONDO_0005070
T5 934-943 Disease denotes pituitary http://purl.obolibrary.org/obo/MONDO_0021156
T6 1220-1222 Disease denotes CF http://purl.obolibrary.org/obo/MONDO_0009061
T7 1862-1864 Disease denotes CF http://purl.obolibrary.org/obo/MONDO_0009061
T8 2077-2079 Disease denotes CF http://purl.obolibrary.org/obo/MONDO_0009061

NCBITAXON

Id Subject Object Predicate Lexical cue db_id
T1 520-524 OrganismTaxon denotes mice 10088
T2 738-743 OrganismTaxon denotes human 9606

Anatomy-MAT

Id Subject Object Predicate Lexical cue mat_id
T1 69-79 Body_part denotes intestinal http://purl.obolibrary.org/obo/MAT_0000043
T2 125-135 Body_part denotes Intestinal http://purl.obolibrary.org/obo/MAT_0000043
T3 174-179 Body_part denotes fetus http://purl.obolibrary.org/obo/MAT_0000399
T4 664-674 Body_part denotes Intestinal http://purl.obolibrary.org/obo/MAT_0000043
T5 887-897 Body_part denotes intestinal http://purl.obolibrary.org/obo/MAT_0000043
T6 934-943 Body_part denotes pituitary http://purl.obolibrary.org/obo/MAT_0000077
T7 1300-1305 Body_part denotes fetus http://purl.obolibrary.org/obo/MAT_0000399
T8 1828-1838 Body_part denotes intestinal http://purl.obolibrary.org/obo/MAT_0000043

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 69-90 Body_part denotes intestinal epithelium http://purl.obolibrary.org/obo/UBERON_0001277
T2 125-152 Body_part denotes Intestinal epithelial cells http://purl.obolibrary.org/obo/CL_0002563
T3 174-179 Body_part denotes fetus http://purl.obolibrary.org/obo/UBERON_0000323
T4 844-852 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T7 934-943 Body_part denotes pituitary http://purl.obolibrary.org/obo/UBERON_0000007
T8 1163-1178 Body_part denotes chorionic villi http://purl.obolibrary.org/obo/UBERON_0007106
T9 1300-1305 Body_part denotes fetus http://purl.obolibrary.org/obo/UBERON_0000323
T10 1726-1734 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158