| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-110 |
Sentence |
denotes |
Chemistry of periodate-mediated cross-linking of 3,4-dihydroxylphenylalanine-containing molecules to proteins. |
| T2 |
111-213 |
Sentence |
denotes |
Chemical cross-linking is an attractive approach to map peptide-protein and protein-protein complexes. |
| T3 |
214-428 |
Sentence |
denotes |
Previously, we explored 3,4-dihydroxylphenylalanine (DOPA) as a protein cross-linking agent upon periodate oxidation (Burdine, L.; Gillette, T. G.; Lin, H.-J.; Kodadek, T. J. Am. Chem. Soc. 2004, 126, 11442-11443). |
| T4 |
429-499 |
Sentence |
denotes |
We report here a study on the chemistry of DOPA-protein cross-linking. |
| T5 |
500-703 |
Sentence |
denotes |
First, using a peptide nucleic acid templated system, we identified the alpha-amino, epsilon-amino of Lys, imidazole of His, and thiol of Cys as functional groups capable of attacking DOPA ortho-quinone. |
| T6 |
704-932 |
Sentence |
denotes |
Second, we demonstrated that periodate-induced DOPA-protein cross-linking could be carried out efficiently at neutral pH in the presence of excess aliphatic 1,2-diols such as ethylene glycol, lactose, and adenosine triphosphate. |
| T7 |
933-1171 |
Sentence |
denotes |
This result indicated that DOPA-protein cross-linking and 1,2-diol oxidative cleavage proceed via different mechanisms and that carbohydrates will not interfere with this process when carried out in crude cell extracts or on intact cells. |
