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PubMed:17111384 JSONTXT

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DisGeNET

Id Subject Object Predicate Lexical cue
T0 654-661 gene:216 denotes ALDH1A1
T1 536-547 disease:C0242379 denotes lung cancer
T2 654-661 gene:216 denotes ALDH1A1
T3 536-547 disease:C0684249 denotes lung cancer
R1 T0 T1 associated_with ALDH1A1,lung cancer
R2 T2 T3 associated_with ALDH1A1,lung cancer

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 237-248 HP_0100526 denotes lung cancer
T2 242-248 HP_0002664 denotes cancer
T3 384-395 HP_0100526 denotes lung cancer
T4 389-395 HP_0002664 denotes cancer
T1 237-248 HP_0100526 denotes lung cancer
T2 242-248 HP_0002664 denotes cancer
T3 384-395 HP_0100526 denotes lung cancer
T4 389-395 HP_0002664 denotes cancer

DisGeNET5_gene_disease

Id Subject Object Predicate Lexical cue
17111384-3#138#145#gene216 654-661 gene216 denotes ALDH1A1
17111384-3#20#31#diseaseC0242379 536-547 diseaseC0242379 denotes lung cancer
17111384-3#20#31#diseaseC0684249 536-547 diseaseC0684249 denotes lung cancer
17111384-3#20#31#diseaseC1306460 536-547 diseaseC1306460 denotes lung cancer
138#145#gene21620#31#diseaseC0242379 17111384-3#138#145#gene216 17111384-3#20#31#diseaseC0242379 associated_with ALDH1A1,lung cancer
138#145#gene21620#31#diseaseC0684249 17111384-3#138#145#gene216 17111384-3#20#31#diseaseC0684249 associated_with ALDH1A1,lung cancer
138#145#gene21620#31#diseaseC1306460 17111384-3#138#145#gene216 17111384-3#20#31#diseaseC1306460 associated_with ALDH1A1,lung cancer

DisGeNet-2017-sample

Id Subject Object Predicate Lexical cue
T816 654-661 gene:216 denotes ALDH1A1
T817 536-547 disease:C0242379 denotes lung cancer
R1 T816 T817 associated_with ALDH1A1,lung cancer
R2 T816 T817 associated_with ALDH1A1,lung cancer
R3 T816 T817 associated_with ALDH1A1,lung cancer

sentences

Id Subject Object Predicate Lexical cue
TextSentencer_T1 0-129 Sentence denotes Heterogeneity of aldehyde dehydrogenase expression in lung cancer cell lines is revealed by Aldefluor flow cytometry-based assay.
TextSentencer_T2 130-141 Sentence denotes BACKGROUND:
TextSentencer_T3 142-249 Sentence denotes We have been interested in studying the roles of two aldehyde dehydrogenases in the biology of lung cancer.
TextSentencer_T4 250-494 Sentence denotes In this study, we seek to apply Aldefluor flow cytometry-based assay for the measurement of aldehyde dehydrogenase (ALDH) activity in lung cancer cell lines, which may become a new tool that will facilitate our continued research in this field.
TextSentencer_T5 495-515 Sentence denotes EXPERIMENTAL DESIGN:
TextSentencer_T6 516-663 Sentence denotes Several established lung cancer cell lines were used, including A549 cell line expressing siRNA against aldehyde dehydrogenase class-1A1 (ALDH1A1).
TextSentencer_T7 664-803 Sentence denotes Western blot analysis, spectrophotometry assay, and Aldefluor staining were used to measure protein or enzyme activity in these cell lines.
TextSentencer_T8 804-1017 Sentence denotes For the purpose of measurement of ALDH activity by Aldefluor in cells with known high ALDH levels, cells were mixed 1:10 with immortalized lung epithelial cell line (Beas-2B), which is known to lack ALDH activity.
TextSentencer_T9 1018-1110 Sentence denotes To delineate dead cells, double staining using Aldefluor and propidium iodide (PI) was done.
TextSentencer_T10 1111-1262 Sentence denotes Double staining was also used to detect changes in ALDH activity in two different cell lines after treatment with 4-hydroperoxycyclophosphamide (4-HC).
TextSentencer_T11 1263-1271 Sentence denotes RESULTS:
TextSentencer_T12 1272-1375 Sentence denotes Our results show a very good correlation between Aldefluor, Western blot, and spectrophotometry assays.
TextSentencer_T13 1376-1620 Sentence denotes Mixing experiments with Beas-2B cells allowed accurate assessment of ALDH activity in A549 cells at baseline and after siRNA expression, thus establishing an approach that facilitates the measurement of very high ALDH using the Aldefluor assay.
TextSentencer_T14 1621-1807 Sentence denotes Aldefluor staining was able to detect heterogeneity in ALDH expression among as well as within the same cell lines and better assess viability after 4-HC treatment when combined with PI.
TextSentencer_T15 1808-1820 Sentence denotes CONCLUSIONS:
TextSentencer_T16 1821-2037 Sentence denotes Aldefluor assay can be adapted successfully to measure ALDH activity in lung cancer cells and may have the advantage of providing real time changes in ALDH activity in viable cells treated with siRNA or chemotherapy.
T1 0-129 Sentence denotes Heterogeneity of aldehyde dehydrogenase expression in lung cancer cell lines is revealed by Aldefluor flow cytometry-based assay.
T2 130-141 Sentence denotes BACKGROUND:
T3 142-249 Sentence denotes We have been interested in studying the roles of two aldehyde dehydrogenases in the biology of lung cancer.
T4 250-494 Sentence denotes In this study, we seek to apply Aldefluor flow cytometry-based assay for the measurement of aldehyde dehydrogenase (ALDH) activity in lung cancer cell lines, which may become a new tool that will facilitate our continued research in this field.
T5 495-515 Sentence denotes EXPERIMENTAL DESIGN:
T6 516-663 Sentence denotes Several established lung cancer cell lines were used, including A549 cell line expressing siRNA against aldehyde dehydrogenase class-1A1 (ALDH1A1).
T7 664-803 Sentence denotes Western blot analysis, spectrophotometry assay, and Aldefluor staining were used to measure protein or enzyme activity in these cell lines.
T8 804-1017 Sentence denotes For the purpose of measurement of ALDH activity by Aldefluor in cells with known high ALDH levels, cells were mixed 1:10 with immortalized lung epithelial cell line (Beas-2B), which is known to lack ALDH activity.
T9 1018-1110 Sentence denotes To delineate dead cells, double staining using Aldefluor and propidium iodide (PI) was done.
T10 1111-1262 Sentence denotes Double staining was also used to detect changes in ALDH activity in two different cell lines after treatment with 4-hydroperoxycyclophosphamide (4-HC).
T11 1263-1271 Sentence denotes RESULTS:
T12 1272-1375 Sentence denotes Our results show a very good correlation between Aldefluor, Western blot, and spectrophotometry assays.
T13 1376-1620 Sentence denotes Mixing experiments with Beas-2B cells allowed accurate assessment of ALDH activity in A549 cells at baseline and after siRNA expression, thus establishing an approach that facilitates the measurement of very high ALDH using the Aldefluor assay.
T14 1621-1807 Sentence denotes Aldefluor staining was able to detect heterogeneity in ALDH expression among as well as within the same cell lines and better assess viability after 4-HC treatment when combined with PI.
T15 1808-1820 Sentence denotes CONCLUSIONS:
T16 1821-2037 Sentence denotes Aldefluor assay can be adapted successfully to measure ALDH activity in lung cancer cells and may have the advantage of providing real time changes in ALDH activity in viable cells treated with siRNA or chemotherapy.

UBERON-AE

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 54-58 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
PD-UBERON-AE-B_T2 237-241 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
PD-UBERON-AE-B_T3 384-388 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
PD-UBERON-AE-B_T4 536-540 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
PD-UBERON-AE-B_T5 943-947 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
PD-UBERON-AE-B_T6 1893-1897 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung

performance-test

Id Subject Object Predicate Lexical cue
PD-UBERON-AE-B_T1 54-58 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
PD-UBERON-AE-B_T2 237-241 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
PD-UBERON-AE-B_T3 384-388 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
PD-UBERON-AE-B_T4 536-540 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
PD-UBERON-AE-B_T5 943-947 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung
PD-UBERON-AE-B_T6 1893-1897 http://purl.obolibrary.org/obo/UBERON_0002048 denotes lung