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PubMed:17110372 JSONTXT

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LitCoin-PubTator-for-Tuning

Id Subject Object Predicate Lexical cue tao:has_database_id
2 9-20 ChemicalEntity denotes cholesterol MESH:D002784
3 39-44 GeneOrGeneProduct denotes ClC-2 Gene:1181
18 89-94 GeneOrGeneProduct denotes ClC-2 Gene:1181
19 236-241 ChemicalEntity denotes lipid MESH:D008055
20 257-262 GeneOrGeneProduct denotes ClC-2 Gene:1181
21 305-316 ChemicalEntity denotes cholesterol MESH:D002784
22 321-326 GeneOrGeneProduct denotes ClC-2 Gene:1181
23 516-521 GeneOrGeneProduct denotes ClC-2 Gene:1181
24 626-637 ChemicalEntity denotes cholesterol MESH:D002784
25 651-675 ChemicalEntity denotes methyl-beta-cyclodextrin MESH:C108732
26 907-931 ChemicalEntity denotes tert-butyl hydroperoxide MESH:D020122
27 942-945 ChemicalEntity denotes ATP MESH:D000255
28 999-1010 ChemicalEntity denotes cholesterol MESH:D002784
29 1053-1064 ChemicalEntity denotes cholesterol MESH:D002784
30 1087-1092 GeneOrGeneProduct denotes ClC-2 Gene:1181
31 1148-1153 GeneOrGeneProduct denotes ClC-2 Gene:1181

LitCoin-PubTator_CellLine

Id Subject Object Predicate Lexical cue cellosaurus_accession_id
T1 465-471 CellLine denotes HEK293 CVCL_0045

sentences

Id Subject Object Predicate Lexical cue
T1 0-88 Sentence denotes Membrane cholesterol content modulates ClC-2 gating and sensitivity to oxidative stress.
T2 89-174 Sentence denotes ClC-2 is a broadly expressed member of the voltage-gated ClC chloride channel family.
T3 175-365 Sentence denotes In this study, we aimed to evaluate the role of the membrane lipid environment in ClC-2 function, and in particular the effect of cholesterol and ClC-2 distribution in membrane microdomains.
T4 366-515 Sentence denotes Detergent-resistant and detergent-soluble microdomains (DSM) were isolated from stably transfected HEK293 cells by a discontinuous OptiPrep gradient.
T5 516-676 Sentence denotes ClC-2 was found concentrated in detergent-insoluble membranes in basal conditions and relocalized to DSM upon cholesterol depletion by methyl-beta-cyclodextrin.
T6 677-805 Sentence denotes As assessed by patch clamp recordings, relocalization was accompanied by acceleration of the activation kinetics of the channel.
T7 806-956 Sentence denotes A similar distribution and activation pattern were obtained when cells were treated with the oxidant tert-butyl hydroperoxide and after ATP depletion.
T8 957-1031 Sentence denotes In both cases activation was prevented by cholesterol enrichment of cells.
T9 1032-1261 Sentence denotes We conclude that the cholesterol environment regulates ClC-2 activity, and we provide evidence that the increase in ClC-2 activity in response to acute oxidative or metabolic stress involves relocalization of this channel to DSM.

PubmedHPO

Id Subject Object Predicate Lexical cue
T1 568-586 HP_0003103 denotes membranes in basal

HP-phenotype

Id Subject Object Predicate Lexical cue hp_id
T1 71-87 Phenotype denotes oxidative stress HP:0025464

Anatomy-UBERON

Id Subject Object Predicate Lexical cue uberon_id
T1 0-8 Body_part denotes Membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T4 227-235 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T7 343-351 Body_part denotes membrane http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158
T10 568-577 Body_part denotes membranes http://purl.obolibrary.org/obo/GO_0016020|http://purl.obolibrary.org/obo/UBERON_0000094|http://purl.obolibrary.org/obo/UBERON_0000158

CL-cell

Id Subject Object Predicate Lexical cue cl_id
T1 581-586 Cell denotes basal http://purl.obolibrary.org/obo/CL:0002324