PubMed:17081065
Annnotations
sentences
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 0-91 | Sentence | denotes | Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes. |
| T2 | 92-280 | Sentence | denotes | Melanin, which is responsible for virtually all visible skin, hair, and eye pigmentation in humans, is synthesized, deposited, and distributed in subcellular organelles termed melanosomes. |
| T3 | 281-399 | Sentence | denotes | A comprehensive determination of the protein composition of this organelle has been obstructed by the melanin present. |
| T4 | 400-540 | Sentence | denotes | Here, we report a novel method of removing melanin that includes in-solution digestion and immobilized metal affinity chromatography (IMAC). |
| T5 | 541-697 | Sentence | denotes | Together with in-gel digestion, this method has allowed us to characterize melanosome proteomes at various developmental stages by tandem mass spectrometry. |
| T6 | 698-891 | Sentence | denotes | Comparative profiling and functional characterization of the melanosome proteomes identified approximately 1500 proteins in melanosomes of all stages, with approximately 600 in any given stage. |
| T7 | 892-1006 | Sentence | denotes | These proteins include 16 homologous to mouse coat color genes and many associated with human pigmentary diseases. |
| T8 | 1007-1141 | Sentence | denotes | Approximately 100 proteins shared by melanosomes from pigmented and nonpigmented melanocytes define the essential melanosome proteome. |
| T9 | 1142-1315 | Sentence | denotes | Proteins validated by confirming their intracellular localization include PEDF (pigment-epithelium derived factor) and SLC24A5 (sodium/potassium/calcium exchanger 5, NCKX5). |
| T10 | 1316-1436 | Sentence | denotes | The sharing of proteins between melanosomes and other lysosome-related organelles suggests a common evolutionary origin. |
| T11 | 1437-1529 | Sentence | denotes | This work represents a model for the study of the biogenesis of lysosome-related organelles. |
| T1 | 0-91 | Sentence | denotes | Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes. |
| T2 | 92-280 | Sentence | denotes | Melanin, which is responsible for virtually all visible skin, hair, and eye pigmentation in humans, is synthesized, deposited, and distributed in subcellular organelles termed melanosomes. |
| T3 | 281-399 | Sentence | denotes | A comprehensive determination of the protein composition of this organelle has been obstructed by the melanin present. |
| T4 | 400-540 | Sentence | denotes | Here, we report a novel method of removing melanin that includes in-solution digestion and immobilized metal affinity chromatography (IMAC). |
| T5 | 541-697 | Sentence | denotes | Together with in-gel digestion, this method has allowed us to characterize melanosome proteomes at various developmental stages by tandem mass spectrometry. |
| T6 | 698-891 | Sentence | denotes | Comparative profiling and functional characterization of the melanosome proteomes identified approximately 1500 proteins in melanosomes of all stages, with approximately 600 in any given stage. |
| T7 | 892-1006 | Sentence | denotes | These proteins include 16 homologous to mouse coat color genes and many associated with human pigmentary diseases. |
| T8 | 1007-1141 | Sentence | denotes | Approximately 100 proteins shared by melanosomes from pigmented and nonpigmented melanocytes define the essential melanosome proteome. |
| T9 | 1142-1315 | Sentence | denotes | Proteins validated by confirming their intracellular localization include PEDF (pigment-epithelium derived factor) and SLC24A5 (sodium/potassium/calcium exchanger 5, NCKX5). |
| T10 | 1316-1436 | Sentence | denotes | The sharing of proteins between melanosomes and other lysosome-related organelles suggests a common evolutionary origin. |
| T11 | 1437-1529 | Sentence | denotes | This work represents a model for the study of the biogenesis of lysosome-related organelles. |
Glycosmos6-MAT
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 148-152 | http://purl.obolibrary.org/obo/MAT_0000284 | denotes | skin |
| T2 | 154-158 | http://purl.obolibrary.org/obo/MAT_0000160 | denotes | hair |
| T3 | 164-167 | http://purl.obolibrary.org/obo/MAT_0000140 | denotes | eye |
| T4 | 168-180 | http://purl.obolibrary.org/obo/MAT_0000159 | denotes | pigmentation |
| T5 | 1061-1070 | http://purl.obolibrary.org/obo/MAT_0000159 | denotes | pigmented |
| T6 | 1222-1229 | http://purl.obolibrary.org/obo/MAT_0000159 | denotes | pigment |
LocText
| Id | Subject | Object | Predicate | Lexical cue |
|---|---|---|---|---|
| T1 | 79-90 | go:GO:0042470 | denotes | melanosomes |
| T2 | 184-190 | taxonomy:9606 | denotes | humans |
| T3 | 268-279 | go:GO:0042470 | denotes | melanosomes |
| T4 | 616-626 | go:GO:0042470 | denotes | melanosome |
| T5 | 759-769 | go:GO:0042470 | denotes | melanosome |
| T6 | 822-833 | go:GO:0042470 | denotes | melanosomes |
| T7 | 932-937 | taxonomy:10090 | denotes | mouse |
| T8 | 980-985 | taxonomy:9606 | denotes | human |
| T9 | 1044-1055 | go:GO:0042470 | denotes | melanosomes |
| T10 | 1121-1131 | go:GO:0042470 | denotes | melanosome |
| T11 | 1216-1220 | uniprot:P36955 | denotes | PEDF |
| T12 | 1222-1255 | uniprot:P36955 | denotes | pigment-epithelium derived factor |
| T13 | 1261-1268 | uniprot:Q71RS6 | denotes | SLC24A5 |
| T14 | 1270-1306 | uniprot:Q71RS6 | denotes | sodium/potassium/calcium exchanger 5 |
| T15 | 1308-1313 | uniprot:Q71RS6 | denotes | NCKX5 |
| T16 | 1348-1359 | go:GO:0042470 | denotes | melanosomes |
| T17 | 1370-1378 | go:GO:0005764 | denotes | lysosome |
| T18 | 1501-1509 | go:GO:0005764 | denotes | lysosome |
| R1 | T11 | T10 | localizeTo | PEDF,melanosome |
| R2 | T12 | T10 | localizeTo | pigment-epithelium derived factor,melanosome |
| R3 | T13 | T10 | localizeTo | SLC24A5,melanosome |
| R4 | T14 | T10 | localizeTo | sodium/potassium/calcium exchanger 5,melanosome |
| R5 | T15 | T10 | localizeTo | NCKX5,melanosome |
| R6 | T14 | T17 | localizeTo | sodium/potassium/calcium exchanger 5,lysosome |
| R7 | T13 | T17 | localizeTo | SLC24A5,lysosome |
| R8 | T12 | T17 | localizeTo | pigment-epithelium derived factor,lysosome |
| R9 | T11 | T17 | localizeTo | PEDF,lysosome |
| R10 | T15 | T17 | localizeTo | NCKX5,lysosome |
Anatomy-MAT
| Id | Subject | Object | Predicate | Lexical cue | mat_id |
|---|---|---|---|---|---|
| T1 | 148-152 | Body_part | denotes | skin | http://purl.obolibrary.org/obo/MAT_0000284 |
| T2 | 154-158 | Body_part | denotes | hair | http://purl.obolibrary.org/obo/MAT_0000160 |
| T3 | 164-167 | Body_part | denotes | eye | http://purl.obolibrary.org/obo/MAT_0000140 |
| T4 | 168-180 | Body_part | denotes | pigmentation | http://purl.obolibrary.org/obo/MAT_0000159 |
| T5 | 1061-1070 | Body_part | denotes | pigmented | http://purl.obolibrary.org/obo/MAT_0000159 |
| T6 | 1222-1229 | Body_part | denotes | pigment | http://purl.obolibrary.org/obo/MAT_0000159 |
NCBITAXON
| Id | Subject | Object | Predicate | Lexical cue | db_id |
|---|---|---|---|---|---|
| T1 | 932-937 | OrganismTaxon | denotes | mouse | 10088|10090 |
| T3 | 980-985 | OrganismTaxon | denotes | human | 9606 |
Anatomy-UBERON
| Id | Subject | Object | Predicate | Lexical cue | uberon_id |
|---|---|---|---|---|---|
| T1 | 79-90 | Body_part | denotes | melanosomes | http://purl.obolibrary.org/obo/GO_0042470 |
| T2 | 148-152 | Body_part | denotes | skin | http://purl.obolibrary.org/obo/UBERON_0000014|http://purl.obolibrary.org/obo/UBERON_0001003|http://purl.obolibrary.org/obo/UBERON_0002097|http://purl.obolibrary.org/obo/UBERON_0002199 |
| T6 | 154-158 | Body_part | denotes | hair | http://purl.obolibrary.org/obo/UBERON_0001037|http://purl.obolibrary.org/obo/UBERON_8420000 |
| T8 | 164-167 | Body_part | denotes | eye | http://purl.obolibrary.org/obo/UBERON_0000019|http://purl.obolibrary.org/obo/UBERON_0000970|http://purl.obolibrary.org/obo/UBERON_0010230 |
| T11 | 250-260 | Body_part | denotes | organelles | http://purl.obolibrary.org/obo/GO_0043226 |
| T12 | 268-279 | Body_part | denotes | melanosomes | http://purl.obolibrary.org/obo/GO_0042470 |
| T13 | 346-355 | Body_part | denotes | organelle | http://purl.obolibrary.org/obo/GO_0043226 |
| T14 | 616-626 | Body_part | denotes | melanosome | http://purl.obolibrary.org/obo/GO_0042470 |
| T15 | 759-769 | Body_part | denotes | melanosome | http://purl.obolibrary.org/obo/GO_0042470 |
| T16 | 822-833 | Body_part | denotes | melanosomes | http://purl.obolibrary.org/obo/GO_0042470 |
| T17 | 1044-1055 | Body_part | denotes | melanosomes | http://purl.obolibrary.org/obo/GO_0042470 |
| T18 | 1088-1099 | Body_part | denotes | melanocytes | http://purl.obolibrary.org/obo/CL_0000148 |
| T19 | 1121-1131 | Body_part | denotes | melanosome | http://purl.obolibrary.org/obo/GO_0042470 |
| T20 | 1181-1194 | Body_part | denotes | intracellular | http://purl.obolibrary.org/obo/GO_0005622 |
| T21 | 1222-1240 | Body_part | denotes | pigment-epithelium | http://purl.obolibrary.org/obo/UBERON_0001782 |
| T22 | 1348-1359 | Body_part | denotes | melanosomes | http://purl.obolibrary.org/obo/GO_0042470 |
| T23 | 1370-1378 | Body_part | denotes | lysosome | http://purl.obolibrary.org/obo/GO_0005764 |
| T24 | 1387-1397 | Body_part | denotes | organelles | http://purl.obolibrary.org/obo/GO_0043226 |
| T25 | 1501-1509 | Body_part | denotes | lysosome | http://purl.obolibrary.org/obo/GO_0005764 |
| T26 | 1518-1528 | Body_part | denotes | organelles | http://purl.obolibrary.org/obo/GO_0043226 |
CL-cell
| Id | Subject | Object | Predicate | Lexical cue | cl_id |
|---|---|---|---|---|---|
| T1 | 1088-1099 | Cell | denotes | melanocytes | http://purl.obolibrary.org/obo/CL:0000148 |