PubMed:17056596 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PubMed/sourceid/17056596","sourcedb":"PubMed","sourceid":"17056596","source_url":"http://www.ncbi.nlm.nih.gov/pubmed/17056596","text":"Sustained activity of calcium release-activated calcium channels requires translocation of mitochondria to the plasma membrane.\nA rise of the intracellular Ca(2+) concentration has multiple signaling functions. Sustained Ca(2+) influx across plasma membrane through calcium release-activated calcium (CRAC) channels is required for T-cell development in the thymus, gene transcription, and proliferation and differentiation of naïve T-cells into armed effectors cells. Intracellular Ca(2+) signals are shaped by mitochondria, which function as a highly dynamic Ca(2+) buffer. However, the precise role of mitochondria for Ca(2+)-dependent T-cell activation is unknown. Here we have shown that mitochondria are translocated to the plasma membrane as a consequence of Ca(2+) influx and that this directed movement is essential to sustain Ca(2+) influx through CRAC channels. The decreased distance between mitochondria and the plasma membrane enabled mitochondria to take up large amounts of inflowing Ca(2+) at the plasma membrane, thereby preventing Ca(2+)-dependent inactivation of CRAC channels and sustaining Ca(2+) signals. Inhibition of kinesin-dependent mitochondrial movement along microtubules abolished mitochondrial translocation and reduced sustained Ca(2+) signals. Our results show how a directed movement of mitochondria is used to control important cellular functions such as Ca(2+)-dependent T-cell activation.","tracks":[{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":332,"end":338},"obj":"Cell"},{"id":"T2","span":{"begin":427,"end":440},"obj":"Cell"},{"id":"T3","span":{"begin":639,"end":645},"obj":"Cell"},{"id":"T4","span":{"begin":1408,"end":1414},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000898"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A4","pred":"cl_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"subj":"T1","pred":"source","obj":"CL-cell"},{"subj":"T2","pred":"source","obj":"CL-cell"},{"subj":"T3","pred":"source","obj":"CL-cell"},{"subj":"T4","pred":"source","obj":"CL-cell"}]},{"project":"LitCoin-PubTator-for-Tuning","denotations":[{"id":"12","span":{"begin":156,"end":162},"obj":"ChemicalEntity"},{"id":"13","span":{"begin":221,"end":227},"obj":"ChemicalEntity"},{"id":"14","span":{"begin":483,"end":489},"obj":"ChemicalEntity"},{"id":"15","span":{"begin":561,"end":567},"obj":"ChemicalEntity"},{"id":"16","span":{"begin":622,"end":628},"obj":"ChemicalEntity"},{"id":"17","span":{"begin":766,"end":772},"obj":"ChemicalEntity"},{"id":"18","span":{"begin":836,"end":842},"obj":"ChemicalEntity"},{"id":"19","span":{"begin":1000,"end":1006},"obj":"ChemicalEntity"},{"id":"20","span":{"begin":1050,"end":1056},"obj":"ChemicalEntity"},{"id":"21","span":{"begin":1112,"end":1118},"obj":"ChemicalEntity"},{"id":"22","span":{"begin":1262,"end":1268},"obj":"ChemicalEntity"},{"id":"23","span":{"begin":1391,"end":1397},"obj":"ChemicalEntity"}],"attributes":[{"id":"A12","pred":"tao:has_database_id","subj":"12","obj":"MESH:D000069285"},{"id":"A13","pred":"tao:has_database_id","subj":"13","obj":"MESH:D000069285"},{"id":"A14","pred":"tao:has_database_id","subj":"14","obj":"MESH:D000069285"},{"id":"A15","pred":"tao:has_database_id","subj":"15","obj":"MESH:D000069285"},{"id":"A16","pred":"tao:has_database_id","subj":"16","obj":"MESH:D000069285"},{"id":"A17","pred":"tao:has_database_id","subj":"17","obj":"MESH:D000069285"},{"id":"A18","pred":"tao:has_database_id","subj":"18","obj":"MESH:D000069285"},{"id":"A19","pred":"tao:has_database_id","subj":"19","obj":"MESH:D000069285"},{"id":"A20","pred":"tao:has_database_id","subj":"20","obj":"MESH:D000069285"},{"id":"A21","pred":"tao:has_database_id","subj":"21","obj":"MESH:D000069285"},{"id":"A22","pred":"tao:has_database_id","subj":"22","obj":"MESH:D000069285"},{"id":"A23","pred":"tao:has_database_id","subj":"23","obj":"MESH:D000069285"},{"subj":"12","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"13","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"14","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"15","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"16","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"17","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"18","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"19","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"20","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"21","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"22","pred":"source","obj":"LitCoin-PubTator-for-Tuning"},{"subj":"23","pred":"source","obj":"LitCoin-PubTator-for-Tuning"}]},{"project":"sentences","denotations":[{"id":"T1","span":{"begin":0,"end":127},"obj":"Sentence"},{"id":"T2","span":{"begin":128,"end":210},"obj":"Sentence"},{"id":"T3","span":{"begin":211,"end":468},"obj":"Sentence"},{"id":"T4","span":{"begin":469,"end":575},"obj":"Sentence"},{"id":"T5","span":{"begin":576,"end":668},"obj":"Sentence"},{"id":"T6","span":{"begin":669,"end":872},"obj":"Sentence"},{"id":"T7","span":{"begin":873,"end":1127},"obj":"Sentence"},{"id":"T8","span":{"begin":1128,"end":1277},"obj":"Sentence"},{"id":"T9","span":{"begin":1278,"end":1426},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"attributes":[{"subj":"T1","pred":"source","obj":"sentences"},{"subj":"T2","pred":"source","obj":"sentences"},{"subj":"T3","pred":"source","obj":"sentences"},{"subj":"T4","pred":"source","obj":"sentences"},{"subj":"T5","pred":"source","obj":"sentences"},{"subj":"T6","pred":"source","obj":"sentences"},{"subj":"T7","pred":"source","obj":"sentences"},{"subj":"T8","pred":"source","obj":"sentences"},{"subj":"T9","pred":"source","obj":"sentences"}]},{"project":"Glycosmos6-MAT","denotations":[{"id":"T1","span":{"begin":358,"end":364},"obj":"http://purl.obolibrary.org/obo/MAT_0000080"}],"attributes":[{"subj":"T1","pred":"source","obj":"Glycosmos6-MAT"}]},{"project":"PubmedHPO","denotations":[{"id":"T1","span":{"begin":1160,"end":1173},"obj":"HP_0001427"},{"id":"T2","span":{"begin":1212,"end":1225},"obj":"HP_0001427"}],"attributes":[{"subj":"T1","pred":"source","obj":"PubmedHPO"},{"subj":"T2","pred":"source","obj":"PubmedHPO"}]},{"project":"NCBITAXON","denotations":[{"id":"T1","span":{"begin":358,"end":364},"obj":"OrganismTaxon"}],"attributes":[{"id":"A1","pred":"db_id","subj":"T1","obj":"49990"},{"subj":"T1","pred":"source","obj":"NCBITAXON"}]},{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":111,"end":126},"obj":"Body_part"},{"id":"T2","span":{"begin":142,"end":155},"obj":"Body_part"},{"id":"T3","span":{"begin":242,"end":257},"obj":"Body_part"},{"id":"T4","span":{"begin":332,"end":338},"obj":"Body_part"},{"id":"T5","span":{"begin":358,"end":364},"obj":"Body_part"},{"id":"T6","span":{"begin":469,"end":482},"obj":"Body_part"},{"id":"T7","span":{"begin":639,"end":645},"obj":"Body_part"},{"id":"T8","span":{"begin":730,"end":745},"obj":"Body_part"},{"id":"T9","span":{"begin":925,"end":940},"obj":"Body_part"},{"id":"T10","span":{"begin":1014,"end":1029},"obj":"Body_part"},{"id":"T11","span":{"begin":1189,"end":1201},"obj":"Body_part"},{"id":"T12","span":{"begin":1408,"end":1414},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/GO_0005886"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/GO_0005622"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/GO_0005886"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/UBERON_0002370"},{"id":"A6","pred":"uberon_id","subj":"T6","obj":"http://purl.obolibrary.org/obo/GO_0005622"},{"id":"A7","pred":"uberon_id","subj":"T7","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A8","pred":"uberon_id","subj":"T8","obj":"http://purl.obolibrary.org/obo/GO_0005886"},{"id":"A9","pred":"uberon_id","subj":"T9","obj":"http://purl.obolibrary.org/obo/GO_0005886"},{"id":"A10","pred":"uberon_id","subj":"T10","obj":"http://purl.obolibrary.org/obo/GO_0005886"},{"id":"A11","pred":"uberon_id","subj":"T11","obj":"http://purl.obolibrary.org/obo/GO_0005874"},{"id":"A12","pred":"uberon_id","subj":"T12","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"subj":"T1","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T2","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T3","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T4","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T5","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T6","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T7","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T8","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T9","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T10","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T11","pred":"source","obj":"Anatomy-UBERON"},{"subj":"T12","pred":"source","obj":"Anatomy-UBERON"}]},{"project":"Anatomy-MAT","denotations":[{"id":"T1","span":{"begin":358,"end":364},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"mat_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MAT_0000080"},{"subj":"T1","pred":"source","obj":"Anatomy-MAT"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"CL-cell","color":"#93ecea","default":true},{"id":"LitCoin-PubTator-for-Tuning","color":"#ecd093"},{"id":"sentences","color":"#b693ec"},{"id":"Glycosmos6-MAT","color":"#93ec9c"},{"id":"PubmedHPO","color":"#ec93a3"},{"id":"NCBITAXON","color":"#93bdec"},{"id":"Anatomy-UBERON","color":"#d8ec93"},{"id":"Anatomy-MAT","color":"#e693ec"}]}]}}