PubMed:16917081 JSON TXT

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sentences

{"project":"sentences","denotations":[{"id":"TextSentencer_T1","span":{"begin":0,"end":135},"obj":"Sentence"},{"id":"TextSentencer_T2","span":{"begin":136,"end":430},"obj":"Sentence"},{"id":"TextSentencer_T3","span":{"begin":431,"end":711},"obj":"Sentence"},{"id":"TextSentencer_T4","span":{"begin":712,"end":796},"obj":"Sentence"},{"id":"TextSentencer_T5","span":{"begin":797,"end":949},"obj":"Sentence"},{"id":"TextSentencer_T6","span":{"begin":950,"end":1171},"obj":"Sentence"},{"id":"TextSentencer_T7","span":{"begin":1172,"end":1291},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":135},"obj":"Sentence"},{"id":"T2","span":{"begin":136,"end":430},"obj":"Sentence"},{"id":"T3","span":{"begin":431,"end":711},"obj":"Sentence"},{"id":"T4","span":{"begin":712,"end":796},"obj":"Sentence"},{"id":"T5","span":{"begin":797,"end":949},"obj":"Sentence"},{"id":"T6","span":{"begin":950,"end":1291},"obj":"Sentence"},{"id":"T1","span":{"begin":0,"end":135},"obj":"Sentence"},{"id":"T2","span":{"begin":136,"end":430},"obj":"Sentence"},{"id":"T3","span":{"begin":431,"end":711},"obj":"Sentence"},{"id":"T4","span":{"begin":712,"end":796},"obj":"Sentence"},{"id":"T5","span":{"begin":797,"end":949},"obj":"Sentence"},{"id":"T6","span":{"begin":950,"end":1171},"obj":"Sentence"},{"id":"T7","span":{"begin":1172,"end":1291},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}

uniprot-human

{"project":"uniprot-human","denotations":[{"id":"T1","span":{"begin":92,"end":102},"obj":"http://www.uniprot.org/uniprot/P09382"},{"id":"T2","span":{"begin":388,"end":394},"obj":"http://www.uniprot.org/uniprot/P09382"},{"id":"T3","span":{"begin":521,"end":526},"obj":"http://www.uniprot.org/uniprot/P09382"},{"id":"T4","span":{"begin":705,"end":710},"obj":"http://www.uniprot.org/uniprot/P09382"},{"id":"T5","span":{"begin":761,"end":766},"obj":"http://www.uniprot.org/uniprot/P09382"},{"id":"T6","span":{"begin":1029,"end":1034},"obj":"http://www.uniprot.org/uniprot/P09382"},{"id":"T7","span":{"begin":1203,"end":1208},"obj":"http://www.uniprot.org/uniprot/P09382"},{"id":"T8","span":{"begin":678,"end":681},"obj":"http://www.uniprot.org/uniprot/P06729"},{"id":"T9","span":{"begin":1109,"end":1111},"obj":"http://www.uniprot.org/uniprot/O14656"},{"id":"T10","span":{"begin":1164,"end":1166},"obj":"http://www.uniprot.org/uniprot/O14656"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}

uniprot-mouse

{"project":"uniprot-mouse","denotations":[{"id":"T1","span":{"begin":92,"end":102},"obj":"http://www.uniprot.org/uniprot/P16045"},{"id":"T2","span":{"begin":388,"end":394},"obj":"http://www.uniprot.org/uniprot/P16045"},{"id":"T3","span":{"begin":521,"end":526},"obj":"http://www.uniprot.org/uniprot/P16045"},{"id":"T4","span":{"begin":705,"end":710},"obj":"http://www.uniprot.org/uniprot/P16045"},{"id":"T5","span":{"begin":761,"end":766},"obj":"http://www.uniprot.org/uniprot/P16045"},{"id":"T6","span":{"begin":1029,"end":1034},"obj":"http://www.uniprot.org/uniprot/P16045"},{"id":"T7","span":{"begin":1203,"end":1208},"obj":"http://www.uniprot.org/uniprot/P16045"},{"id":"T8","span":{"begin":391,"end":394},"obj":"http://www.uniprot.org/uniprot/P47212"},{"id":"T9","span":{"begin":521,"end":524},"obj":"http://www.uniprot.org/uniprot/P47212"},{"id":"T10","span":{"begin":705,"end":708},"obj":"http://www.uniprot.org/uniprot/P47212"},{"id":"T11","span":{"begin":761,"end":764},"obj":"http://www.uniprot.org/uniprot/P47212"},{"id":"T12","span":{"begin":1029,"end":1032},"obj":"http://www.uniprot.org/uniprot/P47212"},{"id":"T13","span":{"begin":1203,"end":1206},"obj":"http://www.uniprot.org/uniprot/P47212"},{"id":"T14","span":{"begin":678,"end":681},"obj":"http://www.uniprot.org/uniprot/P08920"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}

GlycoBiology-NCBITAXON

{"project":"GlycoBiology-NCBITAXON","denotations":[{"id":"T1","span":{"begin":55,"end":61},"obj":"http://purl.bioontology.org/ontology/STY/T051"},{"id":"T2","span":{"begin":194,"end":208},"obj":"http://purl.bioontology.org/ontology/STY/T043"},{"id":"T3","span":{"begin":342,"end":348},"obj":"http://purl.bioontology.org/ontology/STY/T051"},{"id":"T4","span":{"begin":460,"end":465},"obj":"http://purl.bioontology.org/ontology/STY/T025"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T1","span":{"begin":11,"end":30},"obj":"http://purl.obolibrary.org/obo/GO_0016256"},{"id":"T2","span":{"begin":229,"end":248},"obj":"http://purl.obolibrary.org/obo/GO_0016256"},{"id":"T3","span":{"begin":11,"end":30},"obj":"http://purl.obolibrary.org/obo/GO_0006491"},{"id":"T4","span":{"begin":229,"end":248},"obj":"http://purl.obolibrary.org/obo/GO_0006491"},{"id":"T5","span":{"begin":45,"end":54},"obj":"http://purl.obolibrary.org/obo/GO_0023052"},{"id":"T6","span":{"begin":332,"end":341},"obj":"http://purl.obolibrary.org/obo/GO_0023052"},{"id":"T7","span":{"begin":66,"end":88},"obj":"http://purl.obolibrary.org/obo/GO_0006915"},{"id":"T8","span":{"begin":353,"end":375},"obj":"http://purl.obolibrary.org/obo/GO_0006915"},{"id":"T9","span":{"begin":79,"end":88},"obj":"http://purl.obolibrary.org/obo/GO_0006915"},{"id":"T10","span":{"begin":79,"end":88},"obj":"http://purl.obolibrary.org/obo/GO_0097194"},{"id":"T11","span":{"begin":366,"end":375},"obj":"http://purl.obolibrary.org/obo/GO_0006915"},{"id":"T12","span":{"begin":366,"end":375},"obj":"http://purl.obolibrary.org/obo/GO_0097194"},{"id":"T13","span":{"begin":535,"end":544},"obj":"http://purl.obolibrary.org/obo/GO_0000746"},{"id":"T14","span":{"begin":775,"end":795},"obj":"http://purl.obolibrary.org/obo/GO_0051209"},{"id":"T15","span":{"begin":837,"end":839},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T16","span":{"begin":1256,"end":1258},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T17","span":{"begin":1263,"end":1272},"obj":"http://purl.obolibrary.org/obo/GO_0046960"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T1","span":{"begin":510,"end":517},"obj":"http://purl.obolibrary.org/obo/GO_0070026"},{"id":"T2","span":{"begin":545,"end":552},"obj":"http://purl.obolibrary.org/obo/GO_0070026"},{"id":"T3","span":{"begin":667,"end":674},"obj":"http://purl.obolibrary.org/obo/GO_0070026"},{"id":"T4","span":{"begin":1226,"end":1233},"obj":"http://purl.obolibrary.org/obo/GO_0070026"},{"id":"T5","span":{"begin":510,"end":517},"obj":"http://purl.obolibrary.org/obo/GO_0003680"},{"id":"T6","span":{"begin":545,"end":552},"obj":"http://purl.obolibrary.org/obo/GO_0003680"},{"id":"T7","span":{"begin":667,"end":674},"obj":"http://purl.obolibrary.org/obo/GO_0003680"},{"id":"T8","span":{"begin":1226,"end":1233},"obj":"http://purl.obolibrary.org/obo/GO_0003680"},{"id":"T9","span":{"begin":510,"end":517},"obj":"http://purl.obolibrary.org/obo/GO_0017091"},{"id":"T10","span":{"begin":545,"end":552},"obj":"http://purl.obolibrary.org/obo/GO_0017091"},{"id":"T11","span":{"begin":667,"end":674},"obj":"http://purl.obolibrary.org/obo/GO_0017091"},{"id":"T12","span":{"begin":1226,"end":1233},"obj":"http://purl.obolibrary.org/obo/GO_0017091"},{"id":"T13","span":{"begin":510,"end":517},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T14","span":{"begin":545,"end":552},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T15","span":{"begin":667,"end":674},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T16","span":{"begin":1226,"end":1233},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T17","span":{"begin":1226,"end":1238},"obj":"http://purl.obolibrary.org/obo/GO_0005112"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T1","span":{"begin":194,"end":198},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T2","span":{"begin":505,"end":509},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T3","span":{"begin":460,"end":465},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T4","span":{"begin":908,"end":923},"obj":"http://purl.obolibrary.org/obo/GO_0005886"},{"id":"T5","span":{"begin":915,"end":923},"obj":"http://purl.obolibrary.org/obo/GO_0016020"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}

GlyTouCan-IUPAC

GlycoBiology-Motifs

{"project":"GlycoBiology-Motifs","denotations":[{"id":"T1","span":{"begin":11,"end":19},"obj":"http://rdf.glycoinfo.org/glycan/G00027MO"},{"id":"T2","span":{"begin":229,"end":237},"obj":"http://rdf.glycoinfo.org/glycan/G00027MO"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}

Lectin

{"project":"Lectin","denotations":[{"id":"Lectin_T1","span":{"begin":92,"end":102},"obj":"https://acgg.asia/db/lfdb/LfDB0270"},{"id":"Lectin_T2","span":{"begin":379,"end":389},"obj":"https://acgg.asia/db/lfdb/LfDB0270"},{"id":"Lectin_T3","span":{"begin":92,"end":102},"obj":"https://acgg.asia/db/lfdb/LfDB0057"},{"id":"Lectin_T4","span":{"begin":379,"end":389},"obj":"https://acgg.asia/db/lfdb/LfDB0057"},{"id":"Lectin_T5","span":{"begin":837,"end":839},"obj":"https://acgg.asia/db/lfdb/LfDB0227"},{"id":"Lectin_T6","span":{"begin":1256,"end":1258},"obj":"https://acgg.asia/db/lfdb/LfDB0227"},{"id":"Lectin_T7","span":{"begin":1158,"end":1161},"obj":"https://acgg.asia/db/lfdb/LfDB0219"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}

mondo_disease

{"project":"mondo_disease","denotations":[{"id":"T1","span":{"begin":1158,"end":1161},"obj":"Disease"}],"attributes":[{"id":"A1","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0016692"},{"id":"A2","pred":"mondo_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/MONDO_0018687"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}

Anatomy-UBERON

{"project":"Anatomy-UBERON","denotations":[{"id":"T1","span":{"begin":121,"end":134},"obj":"Body_part"},{"id":"T2","span":{"begin":192,"end":198},"obj":"Body_part"},{"id":"T3","span":{"begin":416,"end":429},"obj":"Body_part"},{"id":"T4","span":{"begin":908,"end":923},"obj":"Body_part"},{"id":"T5","span":{"begin":1011,"end":1024},"obj":"Body_part"}],"attributes":[{"id":"A1","pred":"uberon_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A2","pred":"uberon_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A3","pred":"uberon_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL_0000084"},{"id":"A4","pred":"uberon_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/GO_0005886"},{"id":"A5","pred":"uberon_id","subj":"T5","obj":"http://purl.obolibrary.org/obo/CL_0000084"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}

CL-cell

{"project":"CL-cell","denotations":[{"id":"T1","span":{"begin":121,"end":134},"obj":"Cell"},{"id":"T2","span":{"begin":192,"end":198},"obj":"Cell"},{"id":"T3","span":{"begin":416,"end":429},"obj":"Cell"},{"id":"T4","span":{"begin":1011,"end":1024},"obj":"Cell"}],"attributes":[{"id":"A1","pred":"cl_id","subj":"T1","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A2","pred":"cl_id","subj":"T2","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A3","pred":"cl_id","subj":"T3","obj":"http://purl.obolibrary.org/obo/CL:0000084"},{"id":"A4","pred":"cl_id","subj":"T4","obj":"http://purl.obolibrary.org/obo/CL:0000084"}],"text":"Effects of N-glycan processing inhibitors on signaling events and induction of apoptosis in galectin-1-stimulated Jurkat T lymphocytes.\nTo elucidate the role of N-linked glycans in triggering T-cell functions, the effects of the N-glycan processing inhibitors 1-deoxymannojirimycin (1-DMM) and swainsonine (SW) were investigated on signaling events and induction of apoptosis in galectin-1 (gal-1)-stimulated Jurkat T lymphocytes. The treatment of Jurkat E6.1 cells with 1-DMM and SW strongly reduced the cell binding of gal-1-biotin, conjugate binding to cell lysate glycoproteins, and to cluster of differentiation (CD) 3 immunoprecipitates on blots as well as the binding of CD2 and CD3 to immobilized gal-1. The mannosidase inhibitors efficiently decreased gal-1-induced calcium mobilization. Both phases originated from a transient Ca(2+) release of internal stores, and the sustained influx across the plasma membrane was found to be involved. Both inhibitors suppressed in transiently transfected Jurkat T lymphocytes the gal-1-induced expression of the luciferase (luc) reporter gene constructs pNFAT-TA-Luc and pAP1(phorbol-12-myristate-13-acetate [PMA])-TA-Luc. The data provide evidence that gal-1 triggers through binding to N-linked glycans a Ca(2+)-sensitive apoptotic pathway."}