| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-90 |
Sentence |
denotes |
Fluoro-Jade B staining following zymosan microinjection into the spinal cord white matter. |
| T2 |
91-93 |
Sentence |
denotes |
1. |
| T3 |
94-265 |
Sentence |
denotes |
The fluorescein derivate Fluoro-Jade B (FJB), which primarily labels dead or dying neurons, was used to study the acute focal inflammation in the spinal cord white matter. |
| T4 |
266-466 |
Sentence |
denotes |
Inflammation was induced by microinjection of the yeast particulate zymosan to evaluate the biological effects of intraspinal macrophages activation without the confounding effects of physical trauma. |
| T5 |
467-469 |
Sentence |
denotes |
2. |
| T6 |
470-611 |
Sentence |
denotes |
A single bolus of zymosan (Sigma, 75 nL) was stereotaxically injected at the thoracic level into the lateral white matter of rat spinal cord. |
| T7 |
612-734 |
Sentence |
denotes |
A standard Fluoro-Jade B staining protocol was applied to spinal cord sections at 6, 12, 24 h and 2, 4 days postinjection. |
| T8 |
735-890 |
Sentence |
denotes |
Neutral Red, NADPH-diaphorase, Iba1-IR, and DAPI staining protocols accomplished examination of the cells participating in the acute inflammatory response. |
| T9 |
891-893 |
Sentence |
denotes |
3. |
| T10 |
894-1019 |
Sentence |
denotes |
Zymosan caused formation of clearly delineated inflammation lesions localized in the lateral white matter of the spinal cord. |
| T11 |
1020-1221 |
Sentence |
denotes |
Fluoro-Jade B stained cells in the area of inflammation were not observed at 12 h postinjection while mild FJB staining appeared at 24 h and intense staining was observed at 2 and 4 days postinjection. |
| T12 |
1222-1224 |
Sentence |
denotes |
4. |
| T13 |
1225-1449 |
Sentence |
denotes |
This study shows that the acute response to zymosan-induced inflammation in the rat spinal cord white matter causes a gradual appearance of phagocytic microglia/macrophages and delayed FJB staining of the inflammatory cells. |
| T14 |
1450-1452 |
Sentence |
denotes |
5. |
| T15 |
1453-1543 |
Sentence |
denotes |
FJB, a reliable marker of dying neurons, is a more universal agent than formerly believed. |
| T16 |
1544-1814 |
Sentence |
denotes |
One possible explanation for the gradual appearance of FJB-stained cells in the area of inflammation is that specific time is required for sufficient levels of proteins and/or myelin debris of axonal origin to appear in the cytoplasm of phagocytic microglia/macrophages. |
| T1 |
0-90 |
Sentence |
denotes |
Fluoro-Jade B staining following zymosan microinjection into the spinal cord white matter. |
| T2 |
91-93 |
Sentence |
denotes |
1. |
| T3 |
94-265 |
Sentence |
denotes |
The fluorescein derivate Fluoro-Jade B (FJB), which primarily labels dead or dying neurons, was used to study the acute focal inflammation in the spinal cord white matter. |
| T4 |
266-466 |
Sentence |
denotes |
Inflammation was induced by microinjection of the yeast particulate zymosan to evaluate the biological effects of intraspinal macrophages activation without the confounding effects of physical trauma. |
| T5 |
467-469 |
Sentence |
denotes |
2. |
| T6 |
470-611 |
Sentence |
denotes |
A single bolus of zymosan (Sigma, 75 nL) was stereotaxically injected at the thoracic level into the lateral white matter of rat spinal cord. |
| T7 |
612-734 |
Sentence |
denotes |
A standard Fluoro-Jade B staining protocol was applied to spinal cord sections at 6, 12, 24 h and 2, 4 days postinjection. |
| T8 |
735-890 |
Sentence |
denotes |
Neutral Red, NADPH-diaphorase, Iba1-IR, and DAPI staining protocols accomplished examination of the cells participating in the acute inflammatory response. |
| T9 |
891-893 |
Sentence |
denotes |
3. |
| T10 |
894-1019 |
Sentence |
denotes |
Zymosan caused formation of clearly delineated inflammation lesions localized in the lateral white matter of the spinal cord. |
| T11 |
1020-1221 |
Sentence |
denotes |
Fluoro-Jade B stained cells in the area of inflammation were not observed at 12 h postinjection while mild FJB staining appeared at 24 h and intense staining was observed at 2 and 4 days postinjection. |
| T12 |
1222-1224 |
Sentence |
denotes |
4. |
| T13 |
1225-1449 |
Sentence |
denotes |
This study shows that the acute response to zymosan-induced inflammation in the rat spinal cord white matter causes a gradual appearance of phagocytic microglia/macrophages and delayed FJB staining of the inflammatory cells. |
| T14 |
1450-1452 |
Sentence |
denotes |
5. |
| T15 |
1453-1543 |
Sentence |
denotes |
FJB, a reliable marker of dying neurons, is a more universal agent than formerly believed. |
| T16 |
1544-1814 |
Sentence |
denotes |
One possible explanation for the gradual appearance of FJB-stained cells in the area of inflammation is that specific time is required for sufficient levels of proteins and/or myelin debris of axonal origin to appear in the cytoplasm of phagocytic microglia/macrophages. |