Id |
Subject |
Object |
Predicate |
Lexical cue |
T1 |
0-60 |
Sentence |
denotes |
Apolipoprotein E-4Philadelphia (Glu13----Lys,Arg145----Cys). |
T2 |
61-183 |
Sentence |
denotes |
Homozygosity for two rare point mutations in the apolipoprotein E gene combined with severe type III hyperlipoproteinemia. |
T3 |
184-297 |
Sentence |
denotes |
The molecular defect in a 24-year-old white female with severe type III hyperlipoproteinemia has been elucidated. |
T4 |
298-396 |
Sentence |
denotes |
The patient's apolipoprotein (apo) E migrated in the apoE-4 position on isoelectric focusing gels. |
T5 |
397-544 |
Sentence |
denotes |
On sodium dodecyl sulfate-polyacrylamide gel electrophoresis the apoE-4 variant had a smaller apparent molecular weight than apoE-4(Cys112----Arg). |
T6 |
545-680 |
Sentence |
denotes |
Sequence analysis of DNA amplified with the polymerase chain reaction revealed two nucleotide substitutions in the proband's apoE gene. |
T7 |
681-813 |
Sentence |
denotes |
A C to T mutation converted arginine (CGT) at position 145 of the mature protein to cysteine (TGT) thus creating the apoE-2 variant. |
T8 |
814-1008 |
Sentence |
denotes |
A second G to A substitution at amino acid 13 led to the exchange of lysine (AAG) for glutamic acid (GAG), thereby adding 2 positive charge units to the protein and producing the apoE-5 variant. |
T9 |
1009-1158 |
Sentence |
denotes |
Computer analysis of the apoE-4Philadelphia gene revealed that the G to A mutation in exon 3 resulted in the loss of an AvaI restriction enzyme site. |
T10 |
1159-1286 |
Sentence |
denotes |
The second mutation, a C to T substitution in the fourth exon of the apoE gene, eliminated a cleavage site for the enzyme BbvI. |
T11 |
1287-1468 |
Sentence |
denotes |
Using these restriction fragment length polymorphisms as well as DNA sequence analysis we have demonstrated that the patient is homozygous for both point mutations in the apoE gene. |
T1 |
0-60 |
Sentence |
denotes |
Apolipoprotein E-4Philadelphia (Glu13----Lys,Arg145----Cys). |
T2 |
61-183 |
Sentence |
denotes |
Homozygosity for two rare point mutations in the apolipoprotein E gene combined with severe type III hyperlipoproteinemia. |
T3 |
184-297 |
Sentence |
denotes |
The molecular defect in a 24-year-old white female with severe type III hyperlipoproteinemia has been elucidated. |
T4 |
298-396 |
Sentence |
denotes |
The patient's apolipoprotein (apo) E migrated in the apoE-4 position on isoelectric focusing gels. |
T5 |
397-544 |
Sentence |
denotes |
On sodium dodecyl sulfate-polyacrylamide gel electrophoresis the apoE-4 variant had a smaller apparent molecular weight than apoE-4(Cys112----Arg). |
T6 |
545-680 |
Sentence |
denotes |
Sequence analysis of DNA amplified with the polymerase chain reaction revealed two nucleotide substitutions in the proband's apoE gene. |
T7 |
681-813 |
Sentence |
denotes |
A C to T mutation converted arginine (CGT) at position 145 of the mature protein to cysteine (TGT) thus creating the apoE-2 variant. |
T8 |
814-1008 |
Sentence |
denotes |
A second G to A substitution at amino acid 13 led to the exchange of lysine (AAG) for glutamic acid (GAG), thereby adding 2 positive charge units to the protein and producing the apoE-5 variant. |
T9 |
1009-1158 |
Sentence |
denotes |
Computer analysis of the apoE-4Philadelphia gene revealed that the G to A mutation in exon 3 resulted in the loss of an AvaI restriction enzyme site. |
T10 |
1159-1286 |
Sentence |
denotes |
The second mutation, a C to T substitution in the fourth exon of the apoE gene, eliminated a cleavage site for the enzyme BbvI. |
T11 |
1287-1468 |
Sentence |
denotes |
Using these restriction fragment length polymorphisms as well as DNA sequence analysis we have demonstrated that the patient is homozygous for both point mutations in the apoE gene. |